A5082453181- Muscle Physiology and Disorders
- Ion channel regulation and function
- Cellular transport and secretion
- 3D Printing in Biomedical Research
- Autophagy in Disease and Therapy
- Cell Image Analysis Techniques
- Adipose Tissue and Metabolism
- Lipid Membrane Structure and Behavior
- Cardiomyopathy and Myosin Studies
- Cancer Cells and Metastasis
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Muscle activation and electromyography studies
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Amyotrophic Lateral Sclerosis Research
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Sensor Technologies
- Image Processing Techniques and Applications
- Genetic Neurodegenerative Diseases
- Olfactory and Sensory Function Studies
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Biotin and Related Studies
Mannheim University of Applied Sciences
2016-2025
Heidelberg University
2015-2024
University Hospital Heidelberg
2019-2024
University Medical Centre Mannheim
2024
Bernstein Center for Computational Neuroscience Heidelberg-Mannheim
2024
Karlsruhe Institute of Technology
2011-2021
University Hospital Hradec Králové
2021
Charles University
2021
University of Mannheim
2014
University of Maribor
2012
The cellular basis of age-related tissue deterioration remains largely obscure.The ability to activate compensatory mechanisms in response environmental stress is an important factor for survival and maintenance functions.Autophagy activated both under short prolonged required clear the cell dysfunctional organelles altered proteins.We report that specific autophagy inhibition muscle has a major impact on neuromuscular synaptic function and, consequently, strength, ultimately affecting...
Although the importance of mitochondria in patho-physiology has become increasingly evident, it remains unclear whether these organelles play a role Ca2+ handling by skeletal muscle. This undefined situation is mainly due to technical limitations measuring transients reliably during contraction–relaxation cycle. Using two-photon microscopy and genetically expressed “cameleon” sensors, we developed robust system that enables measurement both cytoplasmic mitochondrial vivo. We show here for...
The distribution and function of sympathetic innervation in skeletal muscle have largely remained elusive. Here we demonstrate that neurons make close contact with neuromuscular junctions form a network may functionally couple different targets including blood vessels, motor neurons, fibers. Direct stimulation led to activation postsynaptic β2-adrenoreceptor (ADRB2), cAMP production, import the transcriptional coactivator peroxisome proliferator-activated receptor γ-coactivator 1α (PPARGC1A)...
Abstract Loss of innervation skeletal muscle is a determinant event in several diseases. Although effectors have been identified, the pathways controlling integrated response to denervation remain largely unknown. Here, we demonstrate that PKB/Akt and mTORC1 play important roles regulating homeostasis maintaining neuromuscular endplates after nerve injury. To allow dynamic changes autophagy, activation must be tightly balanced following denervation. Acutely activating or inhibiting impairs...
Three-dimensional cell cultures, such as spheroids and organoids, serve increasingly important models in fundamental applied research start to be used for drug screening purposes. Optical tissue clearing procedures are employed enhance visualization of fluorescence-stained organs, tissues, three-dimensional cultures. To get a more systematic overview about the effects applicability optical on we compared six different / embedding protocols seven types spheroid- chip-based cultures...
Skeletal muscle contraction depends on the release of Ca2+ from sarcoplasmic reticulum (SR), but dynamics SR free concentration ([Ca2+]SR), its modulation by physiological stimuli such as catecholamines, and concomitant changes in cAMP handling have never been directly determined. We used two-photon microscopy imaging GFP-based probes expressed mouse skeletal muscles to monitor, for first time a live animal, [Ca2+]SR cAMP. Our data, which were obtained highly conditions, suggest that...
Removal of ubiquitinated targets by autophagosomes can be mediated receptor molecules, like SQSTM1, in a mechanism referred to as selective autophagy. While cytoplasmic protein aggregates, mitochondria, and bacteria are the best-known autophagy, their role turnover membrane receptors is scarce. We here showed that fasting-induced wasting skeletal muscle involves remodeling neuromuscular junction (NMJ) increasing muscle-type CHRN (cholinergic receptor, nicotinic/nicotinic acetylcholine...
In yeast, Tom22, the central component of TOMM (translocase outer mitochondrial membrane) receptor complex, is responsible for recognition and translocation synthesized precursor proteins, its protein kinase CK2-dependent phosphorylation mandatory complex biogenesis proper import. mammals, biological function CSNK2/CK2 remains vastly elusive it unknown whether CSNK2-dependent subunits has a similar role as that in yeast. To address this issue, we used skeletal muscle-specific...
Neuromuscular junctions are the synapses between motor neurons and skeletal muscle fibers, which mediate voluntary movement. Since neuromuscular also tightly associated with capping function of terminal Schwann cells, these have been classically regarded as tripartite chemical synapses. Although evidences from sympathetic innervation was described approximately a century ago, essential presence functional relevance contribution to maintenance modulation demonstrated only recently. These...
Neuroendocrine secretory granules, the storage organelles for neuropeptides and hormones, are formed at trans-Golgi network, stored inside cell exocytosed upon stimulation. Previously, we have reported that newly granules of PC12 cells transported in a microtubule-dependent manner from network to F-actin-rich cortex, where they undergo short directed movements exhibit homogeneous distribution. Here provide morphological biochemical evidence myosin Va is associated with granules. Expression...
Secretory granules store neuropeptides and hormones exhibit regulated exocytosis upon appropriate cellular stimulation. They are generated in the trans-Golgi network as immature secretory granules, short-lived vesicular intermediates, which undergo a complex poorly understood maturation process. Due to their short half-life low abundance, real-time studies of have not been previously possible. We describe here pulse/chase-like system based on expression human chromogranin B-GFP fusion...
Recent development of in vivo microscopy techniques, including green fluorescent proteins, has allowed the visualization a wide range dynamic processes living cells. For quantitative and visual interpretation such processes, new concepts for time-resolved image analysis continuous time–space are required. Here, we describe versatile fully automated approach consisting four namely highly sensitive object detection, fuzzy logic-based tracking, computer graphical visualization, measurement...
Calcineurin-NFAT signaling has been shown to control activity-dependent muscle gene regulation and induce a program of expression typical slow oxidative fibers. Following Ca2+-calmodulin stimulation, calcineurin dephosphorylates NFAT proteins induces their translocation into the nucleus. However, nuclear never investigated in skeletal vivo. To determine whether NFATc1 nucleocytoplasmic shuttling depends on activity, we transfected fast mouse muscles with plasmids coding for an NFATc1-GFP...
Muscle atrophy is a process of muscle wasting induced under series catabolic stress conditions, such as denervation, disuse, cancer cachexia, heart and renal failure, AIDS, aging. Neuromuscular junctions (NMJs), the synapses between motor neurons fibers undergo major changes in atrophying muscles, ranging from mild morphological alterations to complete disintegration. In this study, we hypothesized that remodeling NMJs could be linked together. To test this, examined if atrophy-promoting E3...
Skin fulfils a plethora of eminent physiological functions ranging from physical barrier over immunity shield to the interface mediating social interaction.Prone several acquired and inherited diseases, skin is therefore major target pharmaceutical cosmetic research.The lack similarity between human animal rising ethical concerns in use models have driven search for novel realistic three-dimensional models.This review provides survey contemporary compares them terms applicability,...
Neuromuscular junction (NMJ) represents the morphofunctional interface between muscle and nerve. Several chronic pathologies such as aging neurodegenerative diseases, including muscular dystrophy amyotrophic lateral sclerosis, display altered NMJ functional denervation. However, triggers molecular mechanisms underlying dismantlement of remain unclear.Here we provide evidence that perturbation in redox signaling cascades, induced by muscle-specific accumulation mutant SOD1G93A transgenic...
Vertebrate neuromuscular junctions (NMJs) have been conceived as tripartite synapses composed of motor neuron, Schwann cell, and muscle fiber. Recent work has shown the presence sympathetic neurons in immediate vicinity NMJs experimental clinical findings suggest that this plays an eminent role adult NMJ biology. The present study examined postnatal development distribution innervation different muscles using immunofluorescence, confocal microscopy, Western blot. This demonstrates proximity...