A5045242992- 3D Printing in Biomedical Research
- Immunotherapy and Immune Responses
- Hair Growth and Disorders
- Tissue Engineering and Regenerative Medicine
- Cell Image Analysis Techniques
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Electrospun Nanofibers in Biomedical Applications
- Dermatology and Skin Diseases
- Cell Adhesion Molecules Research
- Viral Infectious Diseases and Gene Expression in Insects
- Image Processing Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
- Additive Manufacturing and 3D Printing Technologies
- Cancer Research and Treatments
- RNA regulation and disease
- Immune cells in cancer
- Wound Healing and Treatments
- Cellular Mechanics and Interactions
- Cell death mechanisms and regulation
- Organ and Tissue Transplantation Research
- Hippo pathway signaling and YAP/TAZ
- 3D Shape Modeling and Analysis
Mannheim University of Applied Sciences
2017-2025
German Cancer Research Center
2002-2024
Heidelberg University
2006-2024
University Hospital Heidelberg
2009-2017
University Medical Centre Mannheim
2010-2017
Heidelberg University
2015
University of Mannheim
2012-2013
Engineering and Physical Sciences Research Council
2012
The metastasizing capacity of the rat pancreatic adenocarcinoma BSp73ASML (ASMLwt) is strikingly reduced by a knockdown CD44v4-v7 (ASMLkd). We used this model to analyze role CD44 variant isoform (CD44v) in (pre)metastatic niche formation. Intrafootpad injections ASMLwt-, but not ASMLkd-conditioned medium (CM), strongly promote settlement ASMLkd cells lymph nodes and lung. Fractionation CM revealed contribution soluble matrix exosomes, where CD44v6-containing ASMLwt-soluble fraction can...
Three-dimensional cell cultures, such as spheroids and organoids, serve increasingly important models in fundamental applied research start to be used for drug screening purposes. Optical tissue clearing procedures are employed enhance visualization of fluorescence-stained organs, tissues, three-dimensional cultures. To get a more systematic overview about the effects applicability optical on we compared six different / embedding protocols seven types spheroid- chip-based cultures...
Alopecia areata (AA) is a T-cell mediated putative autoimmune disease of hair follicles, which can be transferred by CD4(+) T cells. However, whether T-helper (Th) 1 or Th2 cytokines are predominant has not yet been defined.To elucidate the importance Th1 cells in pathogenesis AA we investigated functional role interferon (IFN)-gamma experimental induction AA.AA was experimentally induced grafting full-thickness skin from AA-affected C3H/HeJ mice on to with targeted deletion cytokine...
Abstract Induction of a chronic eczema is most efficient therapy for alopecia areata (AA). We had noted reduction in regulatory T cells during AA induction and wondered whether may become recruited or expanded repeated skin sensitization additional account hair regrowth. could not be cured by the transfer CD4+CD25high lymph node from mice repeatedly treated with contact sensitizer. This obviously consequence dominance freshly activated as compared CD4+CD25+ cells. Instead, population...
Abstract Biomedical research increasingly relies on three-dimensional (3D) cell culture models and artificial-intelligence-based analysis can potentially facilitate a detailed accurate feature extraction single-cell level. However, this requires for precise segmentation of 3D datasets, which in turn demands high-quality ground truth training. Manual annotation, the gold standard data, is too time-consuming thus not feasible generation large training datasets. To address this, we present...
There is evidence that cell-associated IL-1 alpha supports immune response induction. Here we explored the impact of malignant cell-derived on immunogenicity, induction and tumor-induced immunosuppression using 3-methylcholanthrene-induced fibrosarcoma lines derived from wild-type (wt), alpha-, beta- or IL-1a beta-knockout (IL-1 alpha(-/-), beta(-/-), alphabeta(-/-)) C57BL6 mice. The wt, beta(-/-) alphabeta(-/-) express MHC class I molecules at a high level. do not differ in their...
The analysis of 3D microscopic cell culture images plays a vital role in the development new therapeutics. While cultures offer greater similarity to human organism than adherent cultures, they introduce challenges for automatic evaluation, like increased heterogeneity. Deep learning algorithms are able outperform conventional methods such conditions but require large amount training data. Due data size and complexity, manual annotation generate datasets is nearly impossible task. We...
Abstract: Alopecia areata (AA) is a putative autoimmune disease of the skin with an inflammatory component that can be treated by local application contact sensitizers. Here, we explored whether responsiveness toward diphenylcyclopropenone (DPCP) reflected composition and activation state peripheral blood mononuclear cells (PBMCs). PBMCs 43 AA patients, 26 17 untreated, 31 healthy volunteers were tested. patients' differed from donors slight increase in CD16‐ tumor necrosis factor‐α...
Analyzing the growth of fibrosarcoma lines derived from IL-1α–, IL-1β–, or IL-1αβ–knockout (−/−) mice in immunocompetent host revealed that tumor-derived IL-1α and IL-1β exert strong opposing effects on immune response induction, which prohibited evaluation a potential impact tumorigenicity. Therefore, vivo IL-1–deficient tumor was evaluated nu/nu compared with vitro characteristics. All grow immunocompromised mice. However, IL-1α−/−β–competent (comp) more aggressively, efficiently induce...
Abstract CD44 plays an important role in leukocyte extravasation, which is fortified autoimmune diseases and delayed-type hypersensitivity (DTH) reactions. There additional evidence that distinct isoforms interfere with the extravasation of selective subsets. We wanted to explore this question alopecia areata (AA), a hair-follicle centric disease, chronic eczema. The became interest because AA treated efficiently by topical application contact sensitizer, such mild DTH reaction maintained...
Abstract Regain of immunocompetence after myeloablation and bone marrow cell (BMC) reconstitution essentially depends on T progenitor homing into the thymus intrathymic maturation. CD44 facilitates settlement in is known as a marker. In search for improving regain BMC reconstitution, we explored whether standard (CD44 s) and/or variant isoforms CD44v6 CD44v7 contribute to repopulation thymocyte Antibody-blocking studies cells/mice with targeted deletion CD44v6/7 or revealed that CD44s, but...
When developing tissue engineered solutions for existing clinical problems, cell seeding strategies should be optimized desired distribution within matrices. The purpose of this investigation was to compare the effects different static methods and subsequent culture up 12 days with regard efficiency resulting cellular in acellular dermis.The tested were surface both unmodified mechanically incised dermis, syringe injection suspension, application low-pressure use an ultrasonic bath remove...
The structure of a biological scaffold is major determinant its characteristics and interaction with cells. An acellular dermis tissue transplant must undergo series processing steps, to remove cells genetic material provide the sterility required for surgical use. During manufacturing sterilization composition transplants may change. human cell-free Epiflex® was investigated specific attention paid structure, matrix composition, cellular content biomechanics. We demonstrated that after...
The anti-apoptotic cellular FLICE-like inhibitory protein cFLIP plays a pivotal role in normal tissues homoeostasis and the development of many tumors, but its thymus (NT), thymomas thymic carcinomas (TC) is largely unknown. Expression, regulation function were analyzed biopsies NT, thymomas, squamous cell (TSCC), epithelial cells (TECs) derived thereof TC line 1889c by qRT-PCR, western blot, shRNA techniques, functional assays addressing survival, senescence autophagy. More than 90% TSCCs...
The acute phase of alopecia areata (AA) is characterized by an increase in CD44v3+ and CD44v10+ skin-infiltrating leucocytes (SkIL). Induction a contact eczema, one the therapeutic options AA, can be mitigated strongly blockade CD44v10. observation that induction delayed type hypersensitivity (DTH) reaction abrogates autoimmune reaction, where both responses apparently use similar effector mechanisms, surprising prompted us to search for underlying mechanisms. AA-affected C3H/HeJ mice were...
INTRODUCTION:In recent years, many different methods were introduced for generation of 3D cell culture.However, currently available three-dimensional techniques are not suitable certain lines and sometimes showed a lack reproducibility.Therefore, specific protocols needed.In this work, we demonstrate generating culture SCC4 tongue cancer line discuss their applicability.MATERIALS AND METHODS: Using three methods, tumor spheroids generated from cells cultured 20 days.To investigate the...
Abstract Spheroids have become principal three-dimensional biological models to study cancer, developmental processes, and drug efficacy. For spheroid generation, ultra-low attachment plates are noteworthy due their simplicity, compatibility with automation, experimental commercial accessibility. Nonetheless, it is unknown whether what degree the plate type impacts formation biology. This employed automated brightfield microscopy systematically compare size eccentricity of spheroids formed...