A5030844665- Advanced Glycation End Products research
- Redox biology and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Biochemical effects in animals
- Monoclonal and Polyclonal Antibodies Research
- Muscle Physiology and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Protein purification and stability
- Autophagy in Disease and Therapy
- Skin Protection and Aging
- Glycosylation and Glycoproteins Research
- Telomeres, Telomerase, and Senescence
- Connexins and lens biology
- Muscle metabolism and nutrition
- Peptidase Inhibition and Analysis
- Sulfur Compounds in Biology
- Circadian rhythm and melatonin
- Protein Structure and Dynamics
- melanin and skin pigmentation
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Exercise and Physiological Responses
- Protease and Inhibitor Mechanisms
Inserm
2015-2024
Sorbonne Université
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Institut de Biologie Paris-Seine
2015-2024
Adaptation Biologique et Vieillissement
2015-2024
Université Sorbonne Nouvelle
2022
Clinical Research Management
2022
Université Paris Cité
2003-2015
University of Monastir
2015
Université Toulouse III - Paul Sabatier
2015
The widely accepted oxidative stress theory of aging postulates that results from accumulation damage. Surprisingly, data the longest-living rodent known, naked mole-rats [MRs; mass 35 g; maximum lifespan (MLSP) > 28.3 years], when compared with mice (MLSP 3.5 years) exhibit higher levels lipid peroxidation, protein carbonylation, and DNA damage even at a young age. We hypothesize age-related changes in structural stability, oxidation, degradation are abrogated over MR. performed...
The cellular basis of age-related tissue deterioration remains largely obscure.The ability to activate compensatory mechanisms in response environmental stress is an important factor for survival and maintenance functions.Autophagy activated both under short prolonged required clear the cell dysfunctional organelles altered proteins.We report that specific autophagy inhibition muscle has a major impact on neuromuscular synaptic function and, consequently, strength, ultimately affecting...
Abstract Cellular senescence affects many physiological and pathological processes is characterized by durable cell cycle arrest, an inflammatory secretory phenotype metabolic reprogramming. Here, using dynamic transcriptome metabolome profiling in human fibroblasts with different subtypes of senescence, we show that a homoeostatic switch results glycerol-3-phosphate (G3P) phosphoethanolamine (pEtN) accumulation links lipid metabolism to the gene expression programme. Mechanistically,...
Restoration of blood flow to ischemic myocardial tissue results in an increase the production oxygen radicals. Highly reactive, free radical species have potential damage cellular components. Clearly, maintenance viability is dependent, part, on removal altered protein. The proteasome a major intracellular proteolytic system which degrades oxidized and ubiquitinated forms Utilizing <i>in vivo</i> rat model, we demonstrate that coronary occlusion/reperfusion resulted declines...
Normal human fibroblasts undergo a limited number of divisions in culture and progressively they reach state irreversible growth arrest, process termed as replicative senescence. The proteasome is the major cellular proteolytic machinery, function which impaired during However, exact causes its malfunction these conditions are unknown. Using WI38 model for senescence we have observed reduced levels proteasomal peptidase activities coupled with increased both oxidized ubiquitinated proteins...
Incubation of glucose-6-phosphate dehydrogenase (Glu-6-PDH) from Leuconostoc mesenteroides with the lipid peroxidation product 4-hydroxy-2-nonenal leads to formation cross-linked protein.This is accompanied by appearance protein-associated fluorescence excitation and emission maxima 340 415 nm, respectively, disappearance histidine lysine residues.Cross-linked protein less susceptible than native Glu-6-PDH proteolysis multicatalytic protease, a multienzymic proteolytic complex involved in...
© 1997 Federation of European Biochemical Societies.
Abstract Two biochemical deficits have been described in the substantia nigra Parkinson's disease, decreased activity of mitochondrial complex I and reduced proteasomal activity. We analysed interactions between these primary mesencephalic cultures. Proteasome inhibitors (epoxomicin, MG132) exacerbated toxicity [rotenone, 1‐methyl‐4‐phenylpyridinium (MPP + )] toxic dopamine analogue 6‐hydroxydopamine, but not II–V or excitotoxins [ N ‐methyl‐ d ‐aspartate (NMDA), kainate]. Rotenone MPP...
Premature senescence of human diploid fibroblasts (HDFs) can be induced by exposures to a variety oxidative stress and DNA damaging agents. In this study we developed robust model UVB-induced premature skin HDFs. After series 10 subcytotoxic (non-proapoptotic) UVB at 250 mJ/cm2, the so-called biomarkers were markedly expressed: growth arrest, senescence-associated beta-galactosidase activity, gene overexpression, deletion in mitochondrial DNA. A set 44 stress- genes found differentially...
For the process of aging in epidermal cells to be characterized, status oxidized and damaged protein accumulation removal by proteasome has been investigated. Modified content activity were assayed lysates from donors different ages. Increased levels proteins, glycated proteins modified lipid peroxidation product 4-hydroxy-2-nonenal observed old donors. At same time, a decline chymotrypsin-like peptidylglutamyl-peptide hydrolase activities was found keratinocytes. This age-related peptidase...
Background— This study was designed to evaluate the effect of arglabin on NLRP3 inflammasome inhibition and atherosclerotic lesion in ApoE 2 Ki mice fed a high-fat Western-type diet. Methods Results— Arglabin purified, its chemical identity confirmed by mass spectrometry. It inhibited, concentration-dependent manner, interleukin (IL)-1β IL-18, but not IL-6 IL-12, production lipopolysaccharide cholesterol crystal–activated cultured mouse peritoneal macrophages, with maximum at ≈50 nmol/L EC...