A5085957154- Redox biology and oxidative stress
- Lipoproteins and Cardiovascular Health
- Protease and Inhibitor Mechanisms
- Cholesterol and Lipid Metabolism
- Atherosclerosis and Cardiovascular Diseases
- International Development and Aid
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Global trade and economics
- Adipokines, Inflammation, and Metabolic Diseases
- S100 Proteins and Annexins
- Antioxidant Activity and Oxidative Stress
- Autophagy in Disease and Therapy
- Global Financial Crisis and Policies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Heat shock proteins research
- Inflammasome and immune disorders
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and biosynthesis
- Peptidase Inhibition and Analysis
- International Business and FDI
- Fatty Acid Research and Health
- Pharmacological Effects of Medicinal Plants
- Cancer, Lipids, and Metabolism
Inserm
2007-2024
Sorbonne Université
2013-2024
Centre National de la Recherche Scientifique
2008-2024
Adaptation Biologique et Vieillissement
2014-2024
Institut de Biologie Paris-Seine
2016-2024
National Heart Lung and Blood Institute
1995-2017
National Institutes of Health
1995-2017
Institut des Maladies Métaboliques et Cardiovasculaires
2017
Universität Ulm
2009-2016
University of Monastir
2015-2016
Specifically designed and functionalized nanoparticles hold great promise for biomedical applications. Yet, the applicability of is critically predetermined by their surface functionalization. Here we demonstrate that amino-functionalized polystyrene (PS-NH(2)) ∼100 nm in diameter, but not carboxyl- or nonfunctionalized particles, trigger NLRP3 inflammasome activation subsequent release proinflammatory interleukin 1β (IL-1β) human macrophages. PS-NH(2) induced time-dependent proton...
The low density lipoproteins (LDL) of human plasma consist a series discrete particle subspecies distinct physicochemical, immunological, and hydrodynamic properties.Such structural differences are intimately linked to the metabolic heterogeneity circulating LDL in vivo.The current studies were designed evaluate compare interaction from normolipidemic subjects with receptor.Plasma d 1.019-1.063glml healthy males fractionated into 15 defined
In this article, we studied the effect of acetyl-11-keto-beta-boswellic acid (AKbetaBA), a natural inhibitor proinflammatory transcription factor NF-kappaB on development atherosclerotic lesions in apolipoprotein E-deficient (apoE-/-) mice.Atherosclerotic were induced by weekly LPS injection apoE-/- mice. alone increased lesion size approximately 100%, and treatment with AKbetaBA significantly reduced it 50%. Moreover, activity was also plaques LPS-injected mice treated AKbetaBA. As...
Background— This study was designed to evaluate the effect of arglabin on NLRP3 inflammasome inhibition and atherosclerotic lesion in ApoE 2 Ki mice fed a high-fat Western-type diet. Methods Results— Arglabin purified, its chemical identity confirmed by mass spectrometry. It inhibited, concentration-dependent manner, interleukin (IL)-1β IL-18, but not IL-6 IL-12, production lipopolysaccharide cholesterol crystal–activated cultured mouse peritoneal macrophages, with maximum at ≈50 nmol/L EC...
Oxidative stress is believed to play a key role in cardiovascular disorders. Thioredoxin (Trx) an oxidative stress-limiting protein with anti-inflammatory and antiapoptotic properties. Here, we analyzed whether Trx-1 might exert atheroprotective effects by promoting macrophage differentiation into the M2 phenotype.Trx-1 at 1 μg/mL induced downregulation of p16(INK4a) significantly promoted polarization macrophages exposed interleukin (IL)-4 15 ng/mL or IL-4/IL-13 (10 each) vitro, as...
A new polymorphism located at position -629 (CETP/-629A/C) in the promoter of cholesteryl ester transfer protein (CETP) gene is described. The -629A allele was associated with lower CETP mass (P<0. 0001) and higher high density lipoprotein cholesterol (P<0.001) than C a sample 536 control subjects from ECTIM study. Transfection studies HepG2 cells luciferase expression vector incorporating 777-bp fragment containing either or showed significantly activity (-25%, P<0.05). By gel-shift assay,...
Background —The accumulation of macrophage-derived foam cells in atherosclerotic lesions correlates with increased local release matrix-degrading metalloproteinases (MMPs) and a thin fibrous cap. The activity these enzymes is controlled by specific tissue inhibitors (TIMPs). Methods Results —Because oxidized low-density lipoprotein (OxLDL) modulates gene expression, we investigated the effect particles on levels MMP-1, MMP-3, MMP-9, TIMP-1, TIMP-2 culture media human monocyte-derived...
Uptake of modified low-density lipoprotein (LDL) by macrophages through scavenger receptors results in lipid droplets accumulation and foam cell formation. Excess deposition has been reported to modulate expression several genes including adipophilin. In this study, we investigated the function adipophilin cholesterol efflux THP-1 macrophages.Adipophilin mRNA was 3.5-fold higher human atherosclerotic plaques compared with healthy areas same arteries. Moreover, presence acetylated LDL...
Background —To define the role of metalloproteinases (MMPs) in development lipid-rich atherosclerotic lesions relation to balance between proteolytic and antiproteolytic activities, we investigated impact adenovirus-mediated elevation circulating levels human tissue inhibitor MMP (TIMP-1) atherosclerosis-susceptible apolipoprotein E–deficient (apoE −/− ) mice. Methods Results —Infusion apoE mice fed a diet with rAd.RSV.TIMP-1 (1×10 11 viral particles) resulted high hepatic expression TIMP-1....
Lecithin cholesterol acyltransferase (LCAT) is a key enzyme which catalyzes the esterification of free present in plasma lipoproteins. In order to evaluate role LCAT HDL metabolism, 6.2-kilobase (kb) fragment consisting 0.851 and 1.134 kb 5'- 3'-flanking regions, as well entire human gene, was utilized develop transgenic mice. Three different mouse lines overexpressing at levels 11-, 14-, 109-fold higher than non-transgenic mice were established. Northern blot hybridization analysis...
Background: Thioredoxin (TRX)-1, a ubiquitous 12-kDa protein, exerts antioxidant and anti-inflammatory effects. In contrast, the truncated form, called TRX80, produced by macrophages induces upregulation of proinflammatory cytokines. TRX80 also promotes differentiation mouse peritoneal human toward M1 phenotype. Methods: TRX1 plasma levels were determined with specific ELISA. A disintegrin metalloproteinase domain-containing protein (ADAM)-10, ADAM-17, ADAM-10 activities measured SensoLyte...
Lipid-laden macrophages present as foam cells may contribute to the hyperthrombotic state of human atherosclerotic lesions by production tissue factor (TF). We investigated effect exogenous nonlipoprotein cholesterol on expression TF monocyte-derived in culture. Nonlipoprotein at 50 micrograms/ml increased activity 4-fold; induction was dose- and time-dependent. Expression positively correlated with free content macrophages, upon inhibition esterification, reflected de novo synthesis...
Lipid accumulation alters macrophage biology and contributes to lipid retention within the vessel wall. In this study, we investigated role of adipophilin on triglyceride lipid‐droplet formation in THP‐1‐derived macrophages (THP‐1 macrophages). presence acetylated low‐density lipoprotein, infected with an adenovirus expressing human showed a 31% increase content greater number droplets compared control cells. Incubation very lipoprotein (VLDL) dramatically increased cellular similarly...
We investigated the effects of a number stimulatory agents on production both cell-associated and extracellular elastase-type enzymes human monocyte-macrophages in vitro modulation such by modification cellular cholesterol content. The included phorbol myristate acetate (PMA) inflammatory mediators, lipopolysaccharide (LPS), opsonized zymosan (OZ), platelet activating factor (PAF). Using synthetic substrate, N-succinyl-trialanyl-paranitroanilide (SANA), we detected elastase-like activity...
Intraperitoneal injection of arglabin (2.5 ng/g body weight, twice daily, 13 weeks) into female human apolipoprotein E<sub>2</sub> gene knock-in (ApoE<sub>2</sub>Ki) mice fed a high-fat Western-type diet (HFD) reduced plasma levels glucose and insulin by ∼20.0% ± 3.5% 50.0% 2.0%, respectively, in comparison with vehicle-treated mice. Immunohistochemical analysis revealed the absence active caspase-3 islet sections from ApoE<sub>2</sub>Ki HFD treated arglabin. In addition,...
Abstract We have studied the underlying molecular defect in a patient presenting with recurrent pancreatitis, hypertriglyceridemia, and virtually undetectable postheparin plasma lipoprotein lipase (LPL) mass activity, who normalized her triglycerides 3 to 6 months after initiation of either medium-chain triglyceride (MCT) oil or ω-3 fatty acid (ω-3-FA) therapy. After treatment, LPL activity ranged from 24% 39% normal specific was (1.0 nmol·ng −1 ·min ). On discontinuation MCT ω-3-FA,...
Oxidative stress and inflammation play a pathogenic role in atherosclerosis. Thioredoxin-1 (Trx-1) is an anti-oxidative, anti-inflammatory protein with atheroprotective effects. However, vivo cleavage of Trx-1 generates truncated pro-inflammatory protein, Trx-80, which compromises the therapeutic use Trx-1. Here we analysed whether thioredoxin-mimetic peptide (TxMP), CB3 might exert anti-inflammatory, effects ApoE2.Ki mice. We synthesized small TxMP, Ac-Cys-Pro-Cys-amide, characterized its...