A5054905654- Pharmacological Effects of Medicinal Plants
- Sarcoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Natural product bioactivities and synthesis
- Protease and Inhibitor Mechanisms
- Bone Metabolism and Diseases
- Complement system in diseases
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- NF-κB Signaling Pathways
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Cancer-related gene regulation
- Immune Response and Inflammation
- CAR-T cell therapy research
- Inflammatory mediators and NSAID effects
- Atherosclerosis and Cardiovascular Diseases
- Asthma and respiratory diseases
- Alzheimer's disease research and treatments
Universität Ulm
2016-2025
Helmholtz-Institute Ulm
2012-2024
Institute of Pharmacology
2007-2022
University Hospital Ulm
2006-2019
Max Planck Institute for Chemical Ecology
2014
Edinburgh Cancer Research
2013
University of Tübingen
2011
Weizmann Institute of Science
2009
Cancer Research UK
2009
European Institute of Oncology
2009
Initially identified as a molecule that regulates the final step of glycolysis, M2 isoform pyruvate kinase (PKM2) was recently reported to have central role in metabolic reprogramming cancer cells well participating cell cycle progression and gene transcription. Despite intensive efforts, intricate molecular mechanisms through which PKM2 tumor remain elusive.The proliferation apoptosis various pancreatic using lentiviral-mediated abrogation were assessed vitro via Western blot flow...
Abstract Expression of proinflammatory cytokines by monocytes is tightly regulated transcription factors such as NF-κB. In this study, we show that, in LPS-stimulated human peripheral monocytes, the pentacyclic triterpenes acetyl-α-boswellic acid (AαBA) and acetyl-11-keto-β-boswellic (AKβBA) down-regulate TNF-α expression. AαBA AKβBA inhibited NF-κB signaling both detected EMSA, well a NF-κB-dependent luciferase gene reporter assay. By contrast, expression driven IFN-stimulated response...
Artemisia annua L. has gained increasing attention for its anticancer activity. However, beside artemisinin, less is known about the possible bioactive ingredients of and respective herbal preparations. We hypothesized that, in addition to preparations might contain multiple with potential activity.MDA-MB-231 triple negative human breast cancer (TNBC) cells along other treatment resistant, metastatic cell lines were used investigate vitro vivo efficacy an extract marketed as a preparation,...
A versatile biopolymer platform for advancing nanodiamonds (NDs) as unique magnetooptic materials biomedical applications is presented here. Precision coatings are designed by chemical reprogramming the functionalities of serum albumin via a straightforward synthesis protocol. Such biopolymers offer high biocompatibility and precise modification with various functional entities due to large number available reactive amino acid residues. Premodification these provides convenient approach...
Acetyl-boswellic acids (acetyl-BA) are pentacyclic triterpenes derived from the gum resin of frankincense. We have previously shown that these compounds effective cytotoxic agents, acting through a mechanism appears to involve inhibition topoisomerase activity. now investigated action acetyl-BA and show more potent inhibitors human topoisomerases I IIalpha than camptothecin, amsacrine or etoposide, respectively. Our data demonstrate and, lesser extent, some other triterpenes, such as...
Signaling through NF-κB has been implicated in the malignant phenotype as well chemoresistance of various cancers. Here we show that natural compounds acetyl-β-boswellic acid and acetyl-11-keto-β-boswellic (AKβBA) inhibit proliferation elicit cell death chemoresistant androgen-independent PC-3 prostate cancer cells vitro vivo. Induction apoptosis was demonstrated cultured by several parameters including mitochondrial cytochrome c release DNA fragmentation. At molecular level these...
The mechanism of proinflammatory activation human monocytes by plasmin is unknown. Here we demonstrate that in primary monocytes, stimulates mitogen-activated protein kinase (MAPK) signaling via phosphorylation MAPK 3/6 (MKK3/6) and p38 triggers subsequent DNA binding transcription factor activator protein-1 (AP-1). AP-1 complex contained phosphorylated c-Jun ATF2, its activity was blocked the inhibitor SB203580. In addition, elicits Janus (JAK)/signal transducer (STAT) signaling, as...
Objective— Fibrinolytic activity is upregulated in atherosclerotic lesions, yet little known about the role of plasmin macrophage function. We postulated a direct effect on human monocyte-derived macrophages. Methods and Results— Plasmin activates macrophages via annexin A2 heterotetramer composed S100A10 with subsequent stimulation Janus kinase JAK1/TYK2 signaling. leads to STAT3 activation, Akt-dependent NF-κB phosphorylation extracellular signal-regulated 1/2 mitogen-activated p38. These...
Engineered nanomaterials are known to enter human cells, often via active endocytosis. Mechanistic details of the interactions between nanoparticles (NPs) with cells still not well enough understood. NP size is a key parameter that controls endocytic mechanism and affects cellular uptake yield. Therefore, we have systematically analyzed fluorescent NPs in range 3.3–100 nm (diameter) by live cells. By using spinning disk confocal microscopy combination quantitative image analysis, studied...
Vegans are at an increased risk for certain micronutrient deficiencies, foremost of vitamin B12. Little is known about the short-term effects dietary change to plant-based nutrition on B12 metabolism. Systemic biomarkers status, namely, serum and holotranscobalamin, may respond quickly a reduced intake To test this hypothesis, 53 healthy omnivore subjects were randomized controlled unsupplemented vegan diet (VD, n = 26) or meat-rich (MD, 27) 4 weeks. Vitamin status was examined by...
Abstract Background The results of recent surveys indicate that more than 50% the German population has experience with complementary and alternative medicine (CAM) or uses CAM regularly. This study investigated usage CAM-related needs hospitalized patients at university medical centres in state Baden-Württemberg, Germany. Methods A multi-centre, paper-based, pseudonymous survey was carried out by members Academic Centre for Complementary Integrative Medicine. Patients all ages, regardless...
1. The arachidonic acid derivative arachidonylethanolamide (anandamide) is an endogeneous ligand of cannabinoid receptors that induces pharmacological actions similar to those cannabinoids such as delta9-tetrahydrocannabinol (THC). We examined whether anandamide can influence excessive neuronal activity by investigating stimulation-induced population spikes and epileptiform in rat hippocampal slices. For this purpose, the effects were compared with synthetic agonist WIN 55,212-2 its inactive...
In this study we investigated the effects of concomitant food intake on bioavailability distinct boswellic acids (BAs) from test preparation BSE-018, a dry extract Boswellia serrata gum resin. randomised, open, single-dose, two-way crossover study, healthy male subjects received three capsules BSE-018 equivalent to 786 mg resin either in fasted state or together with standardised high-fat meal. BA plasma concentrations were analysed for up 60 h after oral dosing by reversed phase HPLC. As...