A5065781126- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Atherosclerosis and Cardiovascular Diseases
- Immune Response and Inflammation
- Immune cells in cancer
- NF-κB Signaling Pathways
- Protein Kinase Regulation and GTPase Signaling
- Complementary and Alternative Medicine Studies
- Cell Image Analysis Techniques
- Heat shock proteins research
- Cytokine Signaling Pathways and Interactions
- Genomics, phytochemicals, and oxidative stress
- Redox biology and oxidative stress
- IL-33, ST2, and ILC Pathways
- Acupuncture Treatment Research Studies
- Force Microscopy Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Single-cell and spatial transcriptomics
Heidelberg University
2014-2023
University Hospital Heidelberg
2014-2023
Institute of Immunology
1997-2019
German Cancer Research Center
1990-2010
DKFZ-ZMBH Alliance
1990-1995
Max Planck Institute of Immunobiology and Epigenetics
1988
Abstract The capacity of tumor cells to form metastatic foci correlates with their ability interact and migrate through endothelial cell layers. This process involves multiple adhesive interactions between the endothelium. Only little is known about molecular nature these during extravasation cells. In human melanoma cells, integrin αvβ3 involved in transendothelial migration its expression metastasis. However, many do not express β3 integrins. Therefore, it remained unclear how undergo...
Bacteria communicate with one another via specialized signaling molecules, known as quorum sensing molecules or autoinducers. The Pseudomonas aeruginosa-derived molecule N-(3-oxododecanoyl)-l-homoserine lactone (AHL-12), however, also activates mammalian cells. As shown previously, AHL-12-induced chemotaxis, up-regulated CD11b expression, and enhanced phagocytosis of polymorphonuclear neutrophils. Circumstantial evidence concurred a receptor for AHL-12, which has been elusive so far. We now...
Cofilin, an actin-depolymerizing protein, is essential for the functional dynamics of actin cytoskeleton and cell viability. In unstimulated human peripheral blood T lymphocytes cofilin phosphorylated localized in cytoplasm. Following co-stimulation through accessory receptors (e.g. CD2 or CD28) – however, not following TCR/CD3 stimulation alone undergoes dephosphorylation. The subcellular localization as well actin-binding activity are regulated by phosphorylation state serine-3. Thus, only...
During adaptive immune responses, T lymphocytes recognize antigenic peptides presented by MHC molecules on antigen-presenting cells (APCs). This recognition results in the formation of a so-called synapse (IS) at T-cell/APC interface, which is crucial for T-cell activation. The molecular composition IS has been extensively studied, but little known about biophysics and interaction forces between APCs. Here, we report measurement APCs employing atomic force microscopy (AFM). For these...
Dendritic cells (DCs) are key components of the adaptive immune system contributing to initiation and regulation T cell responses. continuously scan DCs in lymphoid organs for presence foreign antigen. However, little is known about functional consequences these frequent cell–DC interactions without cognate Here we demonstrate that contacts absence antigen serve an important function, namely, induction a basal activation level required responsiveness subsequent encounters with antigens. This...
The activity and function of T-cells are influenced by the intra- extracellular redox milieu. Oxidative stress induces hypo-responsiveness untransformed T-cells. Vice versa increased glutathione (GSH) levels or decreased reactive oxygen species (ROS) prime T-cell metabolism for inflammation, e.g. in rheumatoid arthritis (RA). Therefore, balancing milieu may represent a promising new option therapeutic immune modulation. Here we show that sulforaphane (SFN), compound derived from plants...
Vegans are at an increased risk for certain micronutrient deficiencies, foremost of vitamin B12. Little is known about the short-term effects dietary change to plant-based nutrition on B12 metabolism. Systemic biomarkers status, namely, serum and holotranscobalamin, may respond quickly a reduced intake To test this hypothesis, 53 healthy omnivore subjects were randomized controlled unsupplemented vegan diet (VD, n = 26) or meat-rich (MD, 27) 4 weeks. Vitamin status was examined by...
Abstract Background The results of recent surveys indicate that more than 50% the German population has experience with complementary and alternative medicine (CAM) or uses CAM regularly. This study investigated usage CAM-related needs hospitalized patients at university medical centres in state Baden-Württemberg, Germany. Methods A multi-centre, paper-based, pseudonymous survey was carried out by members Academic Centre for Complementary Integrative Medicine. Patients all ages, regardless...
Cytokines released during chronic inflammatory diseases induce pro-inflammatory properties in polymorphonuclear neutrophils (PMNs). Here, we describe the development of a subgroup human PMNs expressing CCR5, termed CCR5+ PMNs. Auto- and paracrine tumor necrosis factor (TNF) signaling increases intracellular neutrophil elastase (ELANE) abundance induces extracellular traps formation (NETosis) PMNs, triggering CCR5 amplifies NETosis. Membranous TNF (mTNF) outside-in reactive oxygen species,...
Abstract In vitro protein kinase assays of CD45 immunprecipitates prepared from digitonin lysates resting human T lymphocytes resulted in exclusive tyrosine phosphorylation a 32‐kDa (pp32). Reprecipitation the phosphorylated proteins and Western blot analysis whole immunoprecipitates employing antisera specifically directed at different kinases demonstrated that p56 lck was responsible for pp32. Since also pp32, present data strongly suggest functional complex is formed between CD45, Such...
Resting T lymphocytes that have recognized antigen bound to a major histocompatibility complex molecule with the T-cell receptor require costimulatory signals through accessory receptors, including CD2, CD4, CD8, and CD28, for their clonal growth expression of functional repertoires. Absence costimulation, in contrast, can induce anergy vitro selective tolerance vivo. Here we defined potential intracellular messenger activation which is strictly regulated by mediated receptors: pp19/cofilin,...
The formation of supramolecular activation clusters within the immunological synapse, crucial for sustained signaling and T lymphocyte activation, requires costimulation-dependent reorganization actin cytoskeleton. Here we have identified actin-remodeling protein cofilin as a key player in this process. Cell-permeable peptides that block costimulation-induced cofilin/F-actin interactions untransformed human lymphocytes impair receptor capping synapse at interface between cells...