A5014225383- Sarcoma Diagnosis and Treatment
- Tumors and Oncological Cases
- Lymphoma Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bone Tumor Diagnosis and Treatments
- RNA modifications and cancer
- CAR-T cell therapy research
- Cardiac tumors and thrombi
- Cell Adhesion Molecules Research
- Orthopedic Infections and Treatments
- Bone Metabolism and Diseases
- Vascular Tumors and Angiosarcomas
- Complement system in diseases
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- Renal and related cancers
- Integrated Circuits and Semiconductor Failure Analysis
- Cancer-related gene regulation
- Biochemical Analysis and Sensing Techniques
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Testicular diseases and treatments
- Antimicrobial Peptides and Activities
- Osteomyelitis and Bone Disorders Research
- Neuroblastoma Research and Treatments
Olgahospital
2011-2025
Klinikum Stuttgart
2011-2025
Heidelberg University
2000-2016
University Children's Hospital Zurich
2006
Abstract Rhabdomyosarcoma is a pediatric tumor type, which classified based on histological criteria into two major subgroups, namely embryonal rhabdomyosarcoma and alveolar rhabdomyosarcoma. The majority, but not all, carry the specific PAX3(7)/FKHR-translocation, whereas there no consistent genetic abnormality recognized in To gain additional insight characteristics of these subtypes, we used oligonucleotide microarrays to measure expression profiles group 29 biopsy samples (15...
Bacteria communicate with one another via specialized signaling molecules, known as quorum sensing molecules or autoinducers. The Pseudomonas aeruginosa-derived molecule N-(3-oxododecanoyl)-l-homoserine lactone (AHL-12), however, also activates mammalian cells. As shown previously, AHL-12-induced chemotaxis, up-regulated CD11b expression, and enhanced phagocytosis of polymorphonuclear neutrophils. Circumstantial evidence concurred a receptor for AHL-12, which has been elusive so far. We now...
Summary Polymophonuclear cells (PMN) of healthy donors do not express major histocompatibility complex (MHC) class II antigens or the T‐cell costimulatory molecules CD80 CD86. Expression these receptors, however, is seen in patients with chronic inflammatory diseases. We now report that, by culturing PMN autologous serum, interferon‐γ (IFN‐γ) and granulocyte–macrophage colony‐stimulating factor (GM‐CSF), de novo synthesis MHC II, CD86 could be induced. II‐positive acquired capacity to...
Rhabdomyosarcoma (RMS) is classified into two main subgroups: the embryonal (ERMS) and alveolar (ARMS) form. The majority of ARMSs are associated with specific chromosomal translocations (pARMS). Because ARMS much more aggressive than ERMS, RMS subclassification has clinical relevance. However, diagnosis subgroups on basis histology or molecular biology can be difficult, supplementing diagnostic methods would desirable. aim this study was to establish a panel markers for subgroup...
T2R38 belongs to the family of bitter receptors and was initially detected in cells oral cavity. We now describe expression tumor patients with pancreatic cancer tumor-derived cell lines. is localized predominantly intracellular association lipid droplets, particularly droplet membrane. The receptor can be activated by bona fide ligand for T2R38, phenylthiourea (PTU), N-acetyl-dodecanoyl homoserine (AHL-12), a quorum sensing molecule Pseudomonas aeruginosa, latter only known natural T2R38....
The complement receptor 3 (CR3; CD11b/CD18) is present exclusively on leukocytes, particularly NK cells, monocytes and polymorphonuclear neutrophils. Approximately 10% of peripheral T lymphocytes and, as we found now mainly CD8+ expressed CD11b. Upon stimulation, however, expression CD11b was up-regulated also CD4+ cells. Stimulation cells either bycross-linked anti-CD3 IL-2 or by mononuclear mitogen yielded up to 28% CD11b+ majority CD56. cell lines established from healthy donors were...
Abstract Polymorphonuclear neutrophils (PMNs) produce an abundance of bactericidal and cytotoxic molecules consistent with their role as first-line defense against bacterial infection. PMNs, however, also cause efficient cellular cytotoxicity when targeted through Fc receptors to appropriate antibody-coated target cells. Although this so-called antibody-dependent (ADCC) was described many years ago, the mechanism killing is still elusive. We now have found that PMNs contain perforin granzyme...
Abstract Background Alveolar Rhabdomyosarcomas (RMA) are characterized by chromosomal translocations, fusing the PAX3 or PAX7 gene with FKHR in about 85%. Previous studies have suggested that fusion type is associated prognosis. In order to investigate predictive value of PAX–FKHR status on disease outcome patients RMA treated CWS trials we performed a retrospective analysis. Procedure Between 1986 and 2004, out 446 four consecutive trials, tumor samples from 126 were available for RT‐PCR...
MYCN amplification is an important therapy-stratifying marker in neuroblastoma. Fluorescence situ hybridization with signal detection on the single-cell level allows a critical judgement of intratumoral heterogeneity.The status was investigated by fluorescence at diagnosis and relapse. Heterogeneity defined as simultaneous presence amplified cells (>/=5 per slide) nonamplified within one tumor or sequential change during course disease. Likewise, heterogeneity can be detected between primary...
Implant infections are a major complication in the field of orthopaedics. Bacteria attach to implant-surface and form biofilm-colonies which makes them difficult treat. Not only immune cells exclusively respond bacterial challenges, but also local tissue capable participating defense mechanisms. The aim this study was evaluate role osteoblasts context implant infections.Primary were cultivated stimulated with free-swimming bacteria at 4 °C 37 °C. Supernatants harvested for ELISA expression...
Bone infections of patients with joint replacement by endoprosthesis (so called "periprosthetic infection") pose a severe problem in the field orthopedic surgery. The diagnosis is often difficult, and treatment is, most cases, complicated prolonged. Patients require an implant exchange surgery, as persistent infection accompanying inflammation lead to tissue damage bone degradation consequently, loosening implant. To gain insight into local inflammatory process, expression proinflammatory...
The ETV6-NTRK3 gene fusion is present in the majority of cases infantile fibrosarcoma (IFS) and acts as a potent oncogenic driver. We report very rapid, complete, sustained response an advanced, chemotherapy-refractory, recurrent IFS to targeted treatment with oral tropomyosin receptor kinase (TRK) inhibitor larotrectinib.A male infant born large congenital tongue had tumour surgically resected at age 4 days. Within 2 months, he developed extensive lymph node recurrence that progressed...
Abstract Background The aim of our analysis was the evaluation prognostic impact SYT‐SSX fusion status and histological grading in synovial sarcoma (SS) children adolescents context consistent multimodal treatment strategy CWS (Cooperative Weichteilsarkom Studie; Cooperative Soft Tissue Sarcoma Study Group) comparison with other risk factors. Procedure Between 1986 2006, out 243 patients SS, tumor samples from 84 localized disease were available for RT‐PCR analysis. Outcome depending on...
Abstract Background Outcome of relapsed disease localized rhabdomyosarcoma remains poor. An individual treatment approach considering the initial systemic and risk group was included in Cooperative Weichteilsarkom Studiengruppe (CWS) Guidance. Methods Second‐line chemotherapy (sCHT) ACCTTIVE based on anthracyclines (adriamycin, carboplatin, cyclophosphamide, topotecan, vincristine, etoposide) recommended for patients with low‐ (LR), standard‐ (SR), high‐risk (HR) after without...
Abstract Background The histologic classification of rhabdomyosarcoma (RMS) as alveolar (aRMS) or embryonal (eRMS) is prognostic importance, with the aRMS being associated a worse outcome. Specific gene fusions ( PAX3/7 :: FOXO1 ) found in majority have been recognized markers poor prognosis and are included current risk stratification instead subtypes localized disease. In metastatic disease, independent significance fusion status has not definitively established. objective this analysis...
Abstract Background The possible application of gene fusion transcripts as tumor‐specific noninvasive liquid biopsy biomarkers was investigated in blood plasma from patients with alveolar rhabdomyosarcoma (ARMS) and synovial sarcoma (SS). Methods Patients entered the CWS Soft‐Tissue Sarcoma Registry (SoTiSaR) tumors positive for genes available blood/plasma samples were included our analysis. Cell‐free exosomal RNA extracted used to detect PAX‐FOXO1 or SYT‐SSX by reverse transcription...
Desmoplastic small round cell tumor (DSRCT) is an aggressive cancer that predominantly affects adolescents and young adults, typically developing at sites lined by mesothelium [1, 2]. DSRCT genetically defined a chromosomal translocation fuses the N-terminus of EWS RNA binding protein 1 (EWSR1) to C-terminus Wilms (WT1), forming EWSR1::WT1 [3]. This fusion encodes potent transcription factor only known driver oncogenic transformation in [4]. The lack comprehensive understanding biology...
Summary Persistent, localized Staphylococcus aureus infections, refractory to antibiotic treatment, can result in massive tissue destruction and surgical intervention is often the only therapeutic option. In that context, we investigated patients with S. aureus-induced infection at various sites, apparent as either olecranon bursitis, empyema of knee joint or soft abscess formation. As expected, a prominent leucocyte infiltrate was found, consisting predominantly polymorphonuclear...
Monocytes have the potential to differentiate either macrophages, dendritic cells, or osteoclasts. The microenvironment, particularly cytokines, directs monocyte differentiation. Receptors of NF<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>κ</mml:mi></mml:mrow></mml:math>B (RANK) ligand, tumor necrosis factor (TNF)<mml:math id="M2"><mml:mrow><mml:mi>α</mml:mi></mml:mrow></mml:math>, interleukin- (IL-) 8 be identified as inducers osteoclastogenesis,...
Abstract Background Rhabdomyosarcoma (RMS) diagnosed during the first year of life is reported to have poor outcome. Little known about treatment and outcome data relapsed disease (RD). Methods Characteristics, treatment, 155 patients ≤ 12 months registered within Cooperative Weichteilsarkom Studiengruppe (CWS) between 1981 2016 were evaluated. Results Localized (LD) was in 144 metastatic (MD) 11. The histological diagnosis alveolar (RMA) ( n = 38, 23/25 examined PAX7/3:FOXO1‐positive),...
The receptor activator of NF-κB (RANK) is especially well studied in the context bone remodeling, and RANK its ligand, RANKL, are key molecules induction resorbing osteoclasts. We now report that polymorphonuclear neutrophils (PMNs) contain preformed RANK, stored secretory vesicles specific granules. Upon stimulation PMNs vitro, was translocated to cell membrane. In patients with persistent bacterial infections, surface expression enhanced compared healthy individuals. functional activity...