A5038723979- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Psoriasis: Treatment and Pathogenesis
- Cellular Mechanics and Interactions
- Heat shock proteins research
- Atherosclerosis and Cardiovascular Diseases
- Piperaceae Chemical and Biological Studies
- Advanced Fluorescence Microscopy Techniques
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Redox biology and oxidative stress
- Genomics, phytochemicals, and oxidative stress
- IL-33, ST2, and ILC Pathways
- Monoclonal and Polyclonal Antibodies Research
- S100 Proteins and Annexins
- Viral Infectious Diseases and Gene Expression in Insects
- Marine Biology and Environmental Chemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Miltenyi Biotec (Germany)
2024
Heidelberg University
2016-2022
University Hospital Heidelberg
2019-2022
The activity and function of T-cells are influenced by the intra- extracellular redox milieu. Oxidative stress induces hypo-responsiveness untransformed T-cells. Vice versa increased glutathione (GSH) levels or decreased reactive oxygen species (ROS) prime T-cell metabolism for inflammation, e.g. in rheumatoid arthritis (RA). Therefore, balancing milieu may represent a promising new option therapeutic immune modulation. Here we show that sulforaphane (SFN), compound derived from plants...
The interplay between keratinocytes and immune cells, especially T plays an important role in the pathogenesis of chronic inflammatory skin diseases. During psoriasis, attract cells by releasing chemokines, while skin-infiltrating self-reactive secrete proinflammatory cytokines, e.g., IFNγ IL-17A, that cause epidermal hyperplasia. Similarly, graft-versus-host disease, allogenic IFNγ-producing Th1/Tc1 IL-17-producing Th17/Tc17 are recruited keratinocyte-derived chemokines accumulate skin....
The death receptor CD95 is expressed in every cancer cell, thus providing a promising tool to target cancer. Activation of can, however, lead apoptosis or proliferation. Yet the molecular determinants CD95's mode action remain unclear. Here, we identify an optimal distance between CD95Ligand molecules that enables specific clustering receptor-ligand pairs, leading efficient activation. Surprisingly, activation leads cells vitro and increased tumor growth vivo. We show allowing 3D aggregation...
Abstract Several antitumor therapies work by increasing reactive oxygen species (ROS) within the tumor micromilieu. Here, we reveal that L-plastin (LPL), an established marker, is reversibly regulated ROS-induced thiol oxidation on Cys101, which forms a disulfide bridge with Cys42. LPL reduction mediated Thioredoxin1 (TRX1) system, as shown TRX1 trapping, knockdown and blockade of reductase (TRXR1) auranofin. diminishes its actin-bundling capacity. Ratiometric imaging using LPL-roGFP-Orp1...
Piperlongumine (PL), a natural small molecule derived from the Piper longum Linn plant, has received growing interest as prooxidative drug with promising anticancer properties. Yet, influence of PL on primary human T cells remained elusive. Knowledge this is crucial importance, however, since in particular play critical role tumor control. Therefore, we investigated effects survival and function peripheral blood (PBTs). While was not cytotoxic to PBTs, it interfered several stages cell...
Cytotoxic T-cells (CTLs) play an important role in many immune-mediated inflammatory diseases. Targeting cytotoxicity of CTLs would allow to interfere with tissue destruction. Here we demonstrate that WF-10, a pro-oxidative compound, inhibits CTL-mediated cytotoxicity. WF-10 did not influence early steps target-cell killing, but impaired the ability detach from initial target cell and move second cell. This reduced serial killing was accompanied by stronger enrichment adhesion molecule LFA-1...
Use of chimeric antigen receptor (CAR) T cells to treat B cell lymphoma and leukemia has been remarkably successful. Unfortunately, the therapeutic efficacy CAR against solid tumors is very limited, with immunosuppression by pro-oxidative tumor microenvironment (TME) a major contributing factor. High levels reactive oxygen species are well-tolerated due their elevated expression antioxidant proteins; however, this not case for cells, which consequently become hypo-responsive. The aim study...
In psoriasis and other inflammatory skin diseases, keratinocytes (KCs) secrete chemokines that attract T cells, which, in turn, cause epidermal hyperplasia by secreting proinflammatory cytokines. To date, it remains unclear whether skin-homing particularly memory can also be activated direct cell contact with KCs. this study, we demonstrated the ability of primary human KCs to activate cells directly transmitting costimulatory signals through CD6/CD166/CD318 axis. Interestingly, despite...
<h3>Background</h3> Glioblastoma multiforme (GBM) is the most common primary brain tumor with median overall survival of approximately 15 months from diagnosis. GBM will inevitably relapse following standard-of-care, and therefore has a high unmet need for new therapeutic strategies. Due to degree intra- interpatient heterogeneity, we sought develop clinical strategy focused on use tumor-infiltrating lymphocytes (TILs) treat GBM. TILs have been used decades in solid tumors good safety...