A5052739497- Ubiquitin and proteasome pathways
- Genetics, Aging, and Longevity in Model Organisms
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Telomeres, Telomerase, and Senescence
- Mitochondrial Function and Pathology
- Antioxidant Activity and Oxidative Stress
- Genomics, phytochemicals, and oxidative stress
- Alzheimer's disease research and treatments
- Advanced Glycation End Products research
- Clusterin in disease pathology
- Circadian rhythm and melatonin
- Muscle metabolism and nutrition
- GDF15 and Related Biomarkers
- Nutrition and Health in Aging
- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Retinoids in leukemia and cellular processes
- Skin Protection and Aging
- Protease and Inhibitor Mechanisms
- Histone Deacetylase Inhibitors Research
- Cholinesterase and Neurodegenerative Diseases
- Redox biology and oxidative stress
- DNA Repair Mechanisms
- Enzyme Production and Characterization
National Hellenic Research Foundation
2016-2025
National and Kapodistrian University of Athens
2021
FORTH Institute of Molecular Biology and Biotechnology
2017
Rede de Química e Tecnologia
2017
Université Paris Cité
2000
Délégation Paris 7
2000
A progressive loss of protein homeostasis is characteristic aging and a driver neurodegeneration. To investigate this process quantitatively, we characterized proteome dynamics during brain in the short-lived vertebrate Nothobranchius furzeri combining transcriptomics proteomics. We detected reduction correlation between mRNA, mainly due to post-transcriptional mechanisms that account for over 40% age-regulated proteins. These changes cause stoichiometry several complexes, including...
Normal human fibroblasts undergo a limited number of divisions in culture and progressively they reach state irreversible growth arrest, process termed as replicative senescence. The proteasome is the major cellular proteolytic machinery, function which impaired during However, exact causes its malfunction these conditions are unknown. Using WI38 model for senescence we have observed reduced levels proteasomal peptidase activities coupled with increased both oxidized ubiquitinated proteins...
The proteasome is the major cellular proteolytic machinery responsible for degradation of both normal and damaged proteins. Proteasomes play a fundamental role in retaining homeostasis. Alterations function have been recorded various biological phenomena including aging. We recently shown that decrease activity senescent human fibroblasts relates to down-regulation β-type subunits. In this study we followed our preliminary observation by developing further characterizing number different...
Replicative senescence in human fibroblasts is accompanied with alterations of various biological processes, including the impaired function proteasome. The proteasome responsible for removal both normal and damaged proteins. Due to its latter function, also considered a representative secondary antioxidant cellular mechanism. Nrf2 basic transcription factor regulation response that has been shown regulate several subunits mice. We have established this study proteasome-related undergoing...
Normal human fibroblasts undergo replicative senescence due to both genetic and environmental factors. Senescence aging can be further accelerated by exposure of cells a variety oxidative agents that contribute among other effects the accumulation damaged proteins. The proteasome, multicatalytic nonlysosomal protease, has impaired function during aging, while its increased expression delays in fibroblasts. aim this study was identify natural compounds enhance proteasome activity exhibit...
Protein homeostasis (proteostasis) is one of the nodal points that need to be preserved retain physiologic cellular/organismal balance. The ubiquitin-proteasome system (UPS) responsible for removal both normal and damaged proteins, with proteasome being downstream effector. major cellular protease progressive impairment function during aging senescence. Despite documented age-retarding properties activation in various models, simultaneous enhancement 20S core content, assembly, have never...
Pollen is a bee-product known for its medical properties from ancient times. In our days increasingly used as health food supplement and especially tonic primarily with appeal to the elderly ameliorate effects of ageing. order evaluate chemical composition biological activity Greek pollen which has never been studied before, one sample identified botanical origin sixteen different common plant taxa Greece evaluated.Three extracts pollen, have tested, in whether could induce proteasome...
Proteasomes are constituents of the cellular proteolytic networks that maintain protein homeostasis through regulated proteolysis normal and abnormal (in any way) proteins. Genetically mediated proteasome activation in multicellular organisms has been shown to promote longevity exert antiaggregation activity. In this study, we investigate whether compound-mediated is feasible a organism dissect effects such approach aging Alzheimer's disease (AD) progression.Feeding wild-type Caenorhabditis...
Alzheimer’s disease (AD) is the most common form of dementia. Given link between oxidative stress and AD, many studies focus on identification natural antioxidants against AD. Although their antioxidant capacity important, increasing data suggest that additional activities are related to beneficial effects, including properties amyloid beta (Aβ) aggregation. Sideritis spp. (mountain tea) extracts possess not only activity but also other bioactivities confer neuroprotection. various have been...