A5058804009- Autophagy in Disease and Therapy
- Mosquito-borne diseases and control
- Cellular transport and secretion
- Invertebrate Immune Response Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Studies on Chitinases and Chitosanases
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Insect Resistance and Genetics
- Insect behavior and control techniques
- Air Quality and Health Impacts
- Air Quality Monitoring and Forecasting
- Lysosomal Storage Disorders Research
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Indoor Air Quality and Microbial Exposure
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Vibrio bacteria research studies
- Retinal Development and Disorders
- Machine Learning in Bioinformatics
- Advanced Electron Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
University of Warwick
2015-2024
University of the Aegean
2019-2024
University of Oslo
2007-2014
Oslo University Hospital
2010-2014
Coventry (United Kingdom)
2014
University of Ioannina
2012
Norwegian Cancer Society
2007-2009
National and Kapodistrian University of Athens
1999-2007
In-Q-Tel
2006
Faculty (United Kingdom)
2006
p62 has been proposed to mark ubiquitinated protein bodies for autophagic degradation. We report that the Drosophila melanogaster orthologue, Ref(2)P, is a regulator of aggregation in adult brain. demonstrate Ref(2)P localizes age-induced aggregates as well caused by reduced or proteasomal activity. A similar localization also observed D. models human neurodegenerative diseases. Although atg8a autophagy mutant flies show accumulation ubiquitin- and Ref(2)P-positive aggregates, this abrogated...
Macroautophagy was initially considered to be a nonselective process for bulk breakdown of cytosolic material. However, recent evidence points toward selective mode autophagy mediated by the so-called receptors (SARs). SARs act recognizing and sorting diverse cargo substrates (e.g., proteins, organelles, pathogens) autophagic machinery. Known are characterized short linear sequence motif (LIR-, LRS-, or AIM-motif) responsible interaction between proteins Atg8 family. Interestingly, many...
Atg8-family proteins are the best-studied of core autophagic machinery. They essential for elongation and closure phagophore into a proper autophagosome. Moreover, associated with from initiation process to, or just prior fusion between autophagosomes lysosomes. In addition to their implication in autophagosome biogenesis, they crucial selective autophagy through ability interact receptor necessary specific targeting substrates degradation. past few years it has been revealed that...
The Atg8 family of ubiquitin-like proteins play pivotal roles in autophagy and other processes involving vesicle fusion transport where the lysosome/vacuole is end station. Nuclear are also emerging. Here, we review structural functional features their protein-protein interaction modes model organisms such as yeast, Arabidopsis, C. elegans Drosophila to humans. Although varying number homologs, from one yeast seven humans, more than ten some plants, there a strong evolutionary conservation...
Autophagy is an evolutionarily conserved pathway responsible for degradation of cytoplasmic material via the lysosome. Although autophagy has been reported to contribute cell death, underlying mechanisms remain largely unknown. In this study, we show that controls DNA fragmentation during late oogenesis in Drosophila melanogaster. Inhibition by genetically removing function genes atg1, atg13, and vps34 resulted stage egg chambers contained persisting nurse nuclei without fragmented...
The proteasome is the major cellular proteolytic machinery responsible for degradation of both normal and damaged proteins. Proteasomes play a fundamental role in retaining homeostasis. Alterations function have been recorded various biological phenomena including aging. We recently shown that decrease activity senescent human fibroblasts relates to down-regulation β-type subunits. In this study we followed our preliminary observation by developing further characterizing number different...
Suppression of macroautophagy, due to mutations or through processes linked aging, results in the accumulation cytoplasmic substrates that are normally eliminated by pathway. This is a significant problem long-lived cells like neurons, where pathway defects can result aggregates containing ubiquitinated proteins. The p62/Ref(2)P family proteins involved autophagic clearance protein bodies sequestosomes. These unique structures closely associated with inclusions ubiquitin as well key...
Cytokinesis, the final step of cell division, normally proceeds to completion in living organisms, so that daughter cells physically separate by abscission. In certain tissues and developmental stages, on other hand, cytokinesis process is incomplete, giving rise interconnected syncytia stable intercellular bridges. This evolutionarily conserved physiological occurs female male germline species ranging from insects humans, has also been observed some somatic invertebrates. Stable bridges...
Cytokinesis, the final step of cell division, normally proceeds to completion in living organisms, so that daughter cells physically separate by abscission. In certain tissues and developmental stages, on other hand, cytokinesis process is incomplete, giving rise interconnected syncytia stable intercellular bridges. This evolutionarily conserved physiological occurs female male germline species ranging from insects humans, has also been observed some somatic invertebrates. Stable bridges...
ESCRT proteins were initially isolated in yeast as a single functional set of conserved components controlling endosomal cargo sorting and multivesicular body (MVB) biogenesis. Recent work has suggested that metazoan might have more functionally diverse roles, but the limited availability mutants species other than hampered thorough analysis. Here, we used genetic screening strategy based on both cell-autonomous non-autonomous growth-promotion phenotypes to isolate null mutations nearly half...
Autophagy is a physiological and evolutionarily conserved process maintaining homeostatic functions, such as protein degradation organelle turnover. Accumulating data provide evidence that autophagy also contributes to cell death under certain circumstances, but how this achieved not well known. Herein, we report occurs during developmentally-induced in the female germline, observed germarium middle developmental stages of oogenesis Drosophila melanogaster. Degenerating germline cells...
Age-related impairment of macroautophagy/autophagy and loss cardiac tissue homeostasis contribute significantly to cardiovascular diseases later in life. MTOR (mechanistic target rapamycin kinase) signaling is the most well-known regulator autophagy, cellular homeostasis, longevity. The consists two structurally functionally distinct multiprotein complexes, MTORC1 MTORC2. While well characterized but role MTORC2 aging autophagy remains poorly understood. Here we identified TGFB-INHB/activin...
Abscission is the final step of cytokinesis that involves cleavage intercellular bridge connecting two daughter cells. Recent studies have given novel insight into spatiotemporal regulation and molecular mechanisms controlling abscission in cultured yeast human The living metazoan tissues are however not well understood. Here we show ALIX ESCRT-III component Shrub required for completion during Drosophila female germline stem cell (fGSC) division. Loss or function fGSCs leads to delayed...
Autophagy is a mechanism of cellular self-degradation that very important for homeostasis and differentiation. Components the endosomal sorting complex required transport (ESCRT) machinery are also autophagy completion cytokinesis. Here we show ESCRT-III subunit CHMP4B not only localizes to normal cytokinetic bridges but chromosome micronuclei, latter surrounded by lysosomes autophagosomes. Moreover, can be co-immunoprecipitated with chromatin. Interestingly, mutation associated autosomal...
Selective autophagy is a catabolic route that turns over specific cellular material for degradation by lysosomes, and whose role in the regulation of innate immunity largely unexplored. Here, we show apical kinase Drosophila immune deficiency (IMD) pathway Tak1, as well its co-activator Tab2, are both selective substrates interact with protein Atg8a. We also present Atg8a-interacting Sh3px1 downregulation IMD pathway, facilitating targeting Tak1/Tab2 complex to platform through interaction...