A5025718299- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Prion Diseases and Protein Misfolding
- Mesenchymal stem cell research
- Trace Elements in Health
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Neurological diseases and metabolism
- Telomeres, Telomerase, and Senescence
- Pluripotent Stem Cells Research
- Amyotrophic Lateral Sclerosis Research
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Food Industry and Aquatic Biology
- Amino Acid Enzymes and Metabolism
- DNA Repair Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Additive Manufacturing and 3D Printing Technologies
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Bone Tissue Engineering Materials
- Machine Learning in Materials Science
Centre for Research and Technology Hellas
2016-2025
Aristotle University of Thessaloniki
2003-2013
Max Delbrück Center
2009-2013
Protein-protein interactions (PPIs) form a complex network called "interactome" that regulates many functions in the cell. In recent years, there is an increasing accumulation of evidence supporting existence hyperbolic geometry underlying representation systems such as interactome. particular, it has been shown embedding human Protein-Interaction Network (hPIN) space (H2) captures biologically relevant information. Here we explore whether this mapping contains information would allow us to...
Spinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disease caused by the expansion of polyglutamine (polyQ) tract in ATXN1 protein. This thought to be responsible for gradual aggregation mutant protein, which associated with increased cytotoxicity and neuronal cell death. Apart from polyQ tract, other domains are also involved initial events protein such as dimerization domain that promotes oligomerization. interacts various proteins; among them, MED15 significantly enhances...
Abstract Polyglutamine (polyQ) diseases are genetically inherited neurodegenerative disorders. They caused by mutations that result in polyQ expansions of particular proteins. Mutant proteins form intranuclear aggregates, induce cytotoxicity and cause neuronal cell death. Protein interaction data suggest regions modulate interactions between coiled‐coil (CC) domains. In the case disease spinocerebellar ataxia type‐1 (SCA1), interacting with CC domains further enhance aggregation toxicity...
Abstract Expansion of the glutamine tract (poly-Q) in protein huntingtin (HTT) causes neurodegenerative disorder Huntington’s disease (HD). Emerging evidence suggests that mutant HTT (mHTT) disrupts brain development. To gain mechanistic insights into neurodevelopmental impact human mHTT, we engineered male induced pluripotent stem cells to introduce a biallelic or monoallelic 70Q expansion remove poly-Q HTT. The introduction mutation caused aberrant development cerebral organoids with loss...
Proteins with long, pathogenic polyglutamine (polyQ) sequences have an enhanced propensity to spontaneously misfold and self-assemble into insoluble protein aggregates. Here, we identified 21 human proteins that influence polyQ-induced ataxin-1 misfolding proteotoxicity in cell model systems. By analyzing the of these modifiers, discovered a recurrent presence coiled-coil (CC) domains toxicity enhancers, while such were not present suppressors. This suggests CC contribute aggregation-...
Abstract Background Spinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disease caused by polyglutamine expansion in the ataxin‐1 protein resulting neuropathology including mutant aggregation, aberrant neurodevelopment, and mitochondrial dysfunction. Objectives Identify SCA1‐relevant phenotypes patient‐specific fibroblasts SCA1 induced pluripotent stem cells (iPSCs) neuronal cultures. Methods iPSCs were generated differentiated into Protein aggregation morphology evaluated using...
Diabetes is a chronic disease characterized by high levels of blood glucose. Diabetic patients should normalize these in order to avoid short and long term clinical complications. Presently, glucose monitoring dependent on frequent finger pricking enzyme based systems that analyze the drawn blood. Continuous monitors are already market but suffer from technical problems, inaccuracy operation time. A novel approach for continuous development implantable cell-based biosensors emit light...
PrPC, the cellular prion protein, is widely expressed in most tissues, including brain, muscle and gastrointestinal tract, but its physiological role remains unclear. During propagation of transmissible spongiform encephalopathies (TSEs), protein converted to pathological isoform, PrPSc, a process believed be mediated by as-yet-unknown host factors. The identification proteins associated with PrP may provide information about biology prions pathogenesis TSEs. In present work, we report...
In transmissible spongiform encephalopathies (TSEs), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is accumulation an abnormal conformational isoform (PrP(Sc)) host-encoded cellular prion protein (PrP(C)). While precise mechanism PrP(C) to PrP(Sc) conversion not understood, it clear that host expression prerequisite for effective infectious propagation. Although there have been studies on TSEs mammalian little known about TSE fish....
Spinocerebellar ataxia type-1 (SCA1) is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1. These expansions are responsible for protein misfolding and self-assembly into intranuclear inclusion bodies (IIBs) that somehow linked to neuronal death. However, owing lack of a suitable cellular model, the downstream consequences IIB formation yet be resolved. Here, we describe nuclear aggregation model pathogenic human ataxin-1 characterize effects. Using inducible Sleeping...
Abstract The Gateway technology cloning system and transposon represent state‐of‐the‐art laboratory techniques. Combination of these molecular tools allows rapid target genes into expression vectors. Here, we describe a novel technology‐compatible plasmid that combines the advantages recombination with Sleeping Beauty (SB) transposon‐mediated transgene integrations. In our transposition is catalyzed by hyperactive SB100x transposase, provides highly efficient precise integrations host...
Background: Biocompatible materials-topography could be used for the construction of scaffolds allowing three-dimensional (3D) organization human stem cells into functional tissue-like structures with a defined architecture. Methods: Structural characterization an alumina-based substrate was performed through XRD, Brunauer–Emmett–Teller (BET) analysis, scanning electron microscopy (SEM), and wettability measurements. Biocompatibility assessed by measuring proliferation differentiation neural...
Abstract PrP C , the cellular isoform of prion protein, is widely expressed in most tissues, including brain, muscle and gastrointestinal tract. Despite its involvement several bioprocesses, has still no apparent physiological role. During propagation transmissible spongiform encephalopathies (TSE), protein converted to pathological isoform, Sc a process believed be mediated by unknown host factors. The identification proteins associated with may provide information about both biology prions...
Huntington’s disease (HD) is caused by the production of a mutant huntingtin (HTT) with an abnormally long poly-glutamine (polyQ) tract, forming aggregates and inclusions in neurons. Previous work us others has shown that increase or decrease polyQ-triggered can be passive simply due to interaction proteins aggregates. To search for active (functional) effects, which might more effective finding therapies mechanisms HD, we selected among interact HTT total 49 pairs that, while being...
Although new genes can arrive from modes other than duplication, few examples are well characterized. Given high expression in some human brain subregions and a putative link to psychological disorders [e.g., schizophrenia (SCZ)], suggestive of functionality, here we characterize piggyBac transposable element-derived 1 (PGBD1). PGBD1 is nonmonotreme mammal-specific under purifying selection, consistent with functionality. The gene body retains much the original DNA transposon but has...
Mesenchymal stromal cells (MSC) have been suggested to beneficial effects on animal models of traumatic brain injury (TBI), owing their neurotrophic and immunomodulatory properties. Adipose tissue-derived (ASCs) are multipotent MSC that can be harvested with minimally invasive methods, show a high proliferative capacity, low immunogenicity if allogeneic, used in autologous or heterologous settings. In the present study ASCs were genetically labelled using Sleeping Beauty transposon express...
Polyglutamine diseases are fatal neurological disorders that affect the central nervous system. They caused by mutations in disease genes contain CAG trinucleotide expansions their coding regions. These translated into expanded glutamine chains pathological proteins. Mutant proteins induce cytotoxicity, form intranuclear aggregates and cause neuronal cell death specific brain At moment there is no cure for these only symptomatic treatments available. Here, we discuss novel therapeutic...
In the last decade, there has been a notable advancement in diverse bioreactor types catering to various applications. However, conventional bioreactors often exhibit bulkiness and high costs, making them less accessible many researchers laboratory facilities. light of these challenges, this article aims introduce evaluate development do-it-yourself (DIY) 3D printed smart bioreactor, offering cost-effective user-friendly solution for proliferation bioentities, including bacteria human...