A5085019177- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Advanced Breast Cancer Therapies
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Acute Lymphoblastic Leukemia research
- Galectins and Cancer Biology
- Viral-associated cancers and disorders
- RNA modifications and cancer
- Chronic Myeloid Leukemia Treatments
- Gene expression and cancer classification
- Immune Cell Function and Interaction
- Genetic factors in colorectal cancer
- Nutrition, Genetics, and Disease
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Genetics, Bioinformatics, and Biomedical Research
- Education, Psychology, and Social Research
- Sarcoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
Central European Institute of Technology
2014-2024
Central European Institute of Technology – Masaryk University
2014-2024
University Hospital Brno
2006-2024
Masaryk University
2010-2024
Medical University of Vienna
2023
University of Dundee
2023
Czech Academy of Sciences, Institute of Biophysics
2013
Cedars-Sinai Medical Center
2013
Abstract The transcription program that is responsible for the pluripotency of human ESCs (hESCs) believed to be comaintained by exogenous fibroblast growth factor-2 (FGF-2), which activates FGF receptors (FGFRs) and stimulates mitogen-activated protein kinase (MAPK) pathway. However, same pathway stimulated insulin receptors, insulin-like factor 1 epidermal receptors. This mechanism further complicated intracrine signals. Thus, molecular mechanisms FGF-2 promotes undifferentiated hESCs are...
In chronic lymphocytic leukemia (CLL), the worst prognosis is associated with TP53 defects affected patients being potentially directed to alternative treatment. Therapy administration was shown drive selection of new mutations in CLL. Using ultra-deep next-generation sequencing (NGS), we performed a detailed analysis mutations' clonal evolution. We retrospectively analyzed samples that were assessed as TP53-wild-type (wt) by FASAY from 20 mutation detected relapse and 40 remaining TP53-wt...
Abstract The planar cell polarity (PCP) pathway is a conserved that regulates migration and in various contexts. Here we show key PCP components such as Vangl2, Celsr1, Prickle1, FZD3, FZD7, Dvl2, Dvl3, casein kinase 1 (CK1)-ϵ are upregulated B lymphocytes of patients with chronic lymphocytic leukemia (CLL). Elevated levels proteins accumulate advanced stages the disease. Here, required for transendothelial invasion CLL cells high expression genes, PRICKLE1, have less favorable clinical...
The multistep process of TP53 mutation expansion during myeloproliferative neoplasm (MPN) transformation into acute myeloid leukemia (AML) has been documented retrospectively. It is currently unknown how common mutations with low variant allele frequency (VAF) are, whether they are linked to hydroxyurea (HU) cytoreduction, and what disease progression risk carry. Using ultra-deep next-generation sequencing, we examined 254 MPN patients treated HU, interferon alpha-2a or anagrelide 85...
Patients with chronic lymphocytic leukemia (CLL) bearing TP53 mutations experience chemorefractory disease and are therefore candidates for targeted therapy. However, the significance of low-burden <10% variant allele frequency (VAF) remains a matter debate. Herein, we describe clonal evolution scenarios mutations, clinical impact which analyzed in "real-world" CLL cohort. status was assessed by next-generation sequencing (NGS) 511 patients entering first-line treatment chemo- and/or...
Frequent truncation mutations of the histone lysine N-methyltransferase KMT2C have been detected by whole exome sequencing studies in various cancers, including malignancies prostate. However, biological consequences these alterations prostate cancer not yet elucidated.To investigate functional effects mutations, we deleted C-terminal catalytic core motif Kmt2c specifically mouse epithelium. We analysed effect SET domain deletion a Pten-deficient PCa model vivo and large number patients.We...
Abstract Background Prostate cancer develops through malignant transformation of the prostate epithelium in a stepwise, mutation-driven process. Although activator protein-1 transcription factors such as JUN have been implicated potential oncogenic drivers, molecular programs contributing to progression are not fully understood. Methods We analyzed expression clinical samples across different stages and investigated its functional role Pten -deficient mouse model. performed histopathological...
Mesalamine (5-ASA) is widely used for the treatment of ulcerative colitis, a remitting condition characterized by chronic inflammation colon. Knowledge about molecular and cellular targets 5-ASA limited clear understanding its activity in intestinal homeostasis interference with neoplastic progression lacking. We sought to identify pathways interfered 5-ASA, using CRC cell lines different genetic background. Microarray was performed gene expression profile 5-ASA-treated untreated cells...
Mesial temporal lobe epilepsy (mTLE) is a severe neurological disorder characterized by recurrent seizures. mTLE frequently accompanied neurodegeneration in the hippocampus resulting hippocampal sclerosis (HS), most common morphological correlate of drug resistance patients. Incomplete knowledge pathological changes mTLE+HS complicates its therapy. The mechanism underlying may involve abnormal gene expression regulation, including posttranscriptional networks involving microRNAs (miRNAs)....
In chronic lymphocytic leukemia, usually a monoclonal disease, multiple productive immunoglobulin heavy chain gene rearrangements are identified sporadically. Prognostication of such cases based on variable mutational status can be problematic, especially if the different have discordant status. To gain insight into possible biological mechanisms underlying origin rearrangements, we performed comprehensive immunogenetic and immunophenotypic characterization 31 with in cohort 1147 patients...
RNA editing by targeted insertion and deletion of uridine is crucial to generate translatable mRNAs from the cryptogenes mitochondrial genome kinetoplastids. This type consists a stepwise cascade reactions generally proceeding 3' 5' on transcript, resulting in population partially edited as well pre-edited completely molecules for each cryptogene these protozoans. Often, number uridines inserted deleted exceed nucleotides that are genome-encoded. Thus, analysis kinetoplastid transcriptomes...
Early identification of resistant cancer cells is currently a major challenge, as their expansion leads to refractoriness. To capture the dynamics these cells, we made comprehensive analysis disease progression and treatment response in chronic lymphocytic leukemia (CLL) patient using combination single-cell bulk genomic methods. At diagnosis, presented with unfavorable genetic markers, including notch receptor 1 (NOTCH1) mutation loss(11q). The initial subsequent lines did not lead durable...
Abstract Androgen deprivation therapy (ADT) remains a key approach in the treatment of prostate cancer (PCa). However, PCa inevitably relapses and becomes ADT resistant. Besides androgens, there is evidence that thyroid hormone thyroxine (T4) its active form 3,5,3′‐triiodo‐ L ‐thyronine (T3) are involved progression PCa. Epidemiologic evidences show higher incidence men with elevated levels. The binding protein μ‐Crystallin (CRYM) mediates intracellular action by sequestering T3 blocks to...
The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. miRNA profiles are different between infected and non-infected We compare SARS-CoV-2 positive negative samples extracted from human nasopharynx tissue show profiles. explored differentially expressed response tissues 10 SARS-CoV-2-positive SARS-CoV-2-negative patients. were identified by sequencing, expression levels selected validated real-time RT-PCR. 943 conserved...
The clinical course of chronic lymphocytic leukemia (CLL) is highly variable. Patients with unmutated IGHV (U-CLL) usually progress rapidly, whereas patients mutated (M-CLL) have a more indolent disease. expression several genes correlates closely the mutational status and could be used to assess prognosis in CLL. We analyzed prognostic relevance COBLL1, LPL, ZAP70 gene expression, which correlated (p < 0.0001), 117 CLL established parameter dividing tested cohort according disease...