There is relatively little information concerning long-term alterations in DNA methylation following exposure of plants to environmental stress. As known about the ratio non-heritable changes and mitotically-inherited changes, dynamics reversibility states were investigated grapevine (Vitis vinifera) stressed by vitro cultivation. It was observed that significant part induced epigenetic could be repeatedly established particular planting stress conditions. However, once conditions...

Several Botryosphaeriaceae species are known to occur worldwide, causing dieback, canker and fruit rot on various hosts. Surveys conducted in ten commercial citrus orchards the northern region of Algeria revealed five belonging three genera associated with diseased trees. Morphological cultural characteristics as well phylogenetic analyses internal transcribed spacer (ITS) translation elongation factor 1-alpha (tef1-α) identified Diplodia mutila, seriata, Dothiorella viticola, Lasiodiplodia...

Arbuscular mycorrhizal fungi (AMF) play a crucial role in enhancing the health and productivity of host plants, including grapevine. By forming symbiotic relationships with plant roots, AMF significantly improve water uptake nutrient absorption, particularly phosphorus (P) nitrogen (N). This study evaluated microbiome composition colonization grapevine endorhizosphere across five wine-growing sub-regions Czech Republic. In all sub-regions, terms fungal microbiome, phyla Ascomycetes...

The NCBI database contains mitochondrial DNA (mtDNA) genomes from numerous species. We investigated the presence and locations of inverted repeat sequences (IRs) in these mtDNA sequences, which are known to be important for regulating nuclear genomes.IRs were identified all IR lengths frequencies correlate with evolutionary age greatest variability was detected subgroups plants fungi lowest mammals. is non-random favoured. frequency IRs generally decreased length, but not 24 or 30 bp long,...

The seed-borne bacterium Xanthomonas campestris pv. (Xcc) as a causal organism of black rot disease remains the most serious bacterial problem agricultural production cruciferous plants worldwide. eradication primary inoculum originating in seeds is available, but no treatment totally effective. With threat developing chemical resistance and increasing pressure for sustainable management, biocontrol methods represent one main strategies currently applied agriculture. Natural antimicrobials,...

The appearance of somaclonal variability induced by in vitro cultivation is relatively frequent and can, some cases, provide a valuable source new genetic variation for crop improvement. cause this phenomenon remains unknown; however, there are number reports suggesting that epigenetics, including DNA methylations, an important factor. In addition to the non-heritable methylation changes caused transient reversible stress-responsive gene regulation, recent evidence supports existence...

The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. miRNA profiles are different between infected and non-infected We compare SARS-CoV-2 positive negative samples extracted from human nasopharynx tissue show profiles. explored differentially expressed response tissues 10 SARS-CoV-2-positive SARS-CoV-2-negative patients. were identified by sequencing, expression levels selected validated real-time RT-PCR. 943 conserved...

A multiplex real-time PCR method based on fluorescent TaqMan® probes was developed for the simultaneous detection of tomato pathogenic bacteria Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. and bacterial spot-causing xanthomonads. The specificity assay validated 44 strains, including 32 target pathogen strains as well closely related species nontarget bacteria. designed showed high sensitivity when positive amplification observed one pg DNA in cases 100 reliability...

Xanthomonas campestris pv. (Xcc) is a bacterium that causes black rot of crucifers. The greatest losses brassica crop production usually result from seed-borne infection, but carry-over inoculum in field soil may also be possible. aim this study was to monitor persistence Xcc central Europe using conventional PCR assay with hrpF primers and two-step nested real-time Zur primers. work has demonstrated can used improve the analytical sensitivity for detection compared PCR, persist up two years...

The study overviews results of bacterial incidence in vitro plant tissue cultures obtained from commercial laboratory dealing with plants micropropagation. For the exploration, 454 pyrosequencing 16S rRNA gene was used. Three samples visual contamination were subjected for identification present bacteria. Eleven genera as Acinetobacter, Lactobacillus, Methylobacterium, Roseomonas, Microbacterium, Mycobacterium, Curtobacterium, Acidovorax, Magnetospirillum, Chryseobacterium and Ralstonia...

Changes in the bacterial spectrum of cabbage heads after storage under commonly used conditions were examined this study. Cabbage seeds (Brassica oleracea var. capitata L.) artificially inoculated with X. campestris pv. (Xcc), a serious pathogen cruciferous plants causing black rot. Isolation cultures from Xcc-inoculated and non-inoculated carried out two time points—at day harvest four months storage. According to our previous research literature reports, most frequent genera bacteria...

p73 is a member of the p53 protein family and has essential functions in several signaling pathways involved development, differentiation, DNA damage responses cancer. As transcription factor, achieves these by binding to consensus sequences shares at least partial target sequence specificity with p53. Transcriptional activation been demonstrated for more than fifty targets yeast and/or human cancer cell lines. It also shown previously that strongly dependent on topology presence inverted...

Tomato is the most consumed vegetable in world. The tomato brown rugose fruit virus (ToBRFV) an important destructive that damages tomatoes and peppers with significant economic impact. detection characterization of this viral pathogen were evaluated at molecular morphological level. isolate was purified inoculated on pepper plants. Small RNAs sequenced both plants profiles compared. complete genome obtained, microRNA (miRNA) unveiled by small RNA sequencing. Symptoms caused also described...

Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers A.J.L. Phillips is a cosmopolitan pathogen causing dieback of multiple diverse woody hosts including highbush blueberry (Vaccinium corymbosum L.). This fungus can survive inside colonized plants without any symptoms for several years. Once the endophytic lifestyle switched to parasitic one, rapidly occur (bronze leaves, necroses under bark, apoplexy) and plant usually declines within few weeks (Slipper Wingfield 2007). In August...

The 14-3-3 protein family is a highly conserved and widely distributed group of proteins consisting multiple isoforms in eukaryotes. Ubiquitously expressed, play key roles DNA replication, cell cycle regulation, apoptosis. function mediated by interaction with large number other DNA. It has been demonstrated that 14-3-3γ binds strongly to cruciform structures crucial for initiating replication. In this study, we analyzed binding properties the isoform linear supercoiled We demonstrate long...

When incubated in a sporulation medium, the sporogenous strains of Bacillus megaterium degrade proteins at rate 4-10% X h-1. The maximal protein turnover is reached after 3-4 hrs time development forespores and then decreases again. asporogenous strain steadily under similar conditions from 3-8% h-1 beginning incubation to 1% 5-6 medium. degradation vitro protoplast lysates or higher than turnover. exocellular, as well periplasmic proteolytic activity, suppressed by amino acids more severely...

Diaporthe species are important pathogens, saprobes, and endophytes on grapevines. Several known, either as agents of pre- or post-harvest infections, causal many relevant diseases, including swelling arm, trunk cankers, leaf spots, root fruit rots, wilts, cane bleaching. A growing body evidence exists that a class small non-coding endogenous RNAs, known microRNAs (miRNAs), play an role in post-transcriptional gene regulation, during plant development responses to biotic abiotic stresses. In...

HomePlant DiseaseVol. 104, No. 5Occurrence of Pseudonectria foliicola Causing Volutella Blight on Boxwood in Czech Republic PreviousNext DISEASE NOTES OPENOpen Access licenseOccurrence RepublicM. Spetik, A. Berraf-Tebbal, J. Cechova, and EichmeierM. Spetik†Corresponding author: M. Spetik; E-mail Address: milan.spetik@mendelu.czhttp://orcid.org/0000-0001-7659-8852Mendeleum – Institute Genetics, Mendel University Brno, 691 44, Lednice na Moravě, Republic, Berraf-TebbalMendeleum...