A5076344113- Genetic factors in colorectal cancer
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- RNA Research and Splicing
- Colorectal Cancer Treatments and Studies
- RNA modifications and cancer
- Erythropoietin and Anemia Treatment
- Platelet Disorders and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer Cells and Metastasis
- Nigella sativa pharmacological applications
- Mechanisms of cancer metastasis
- Blood properties and coagulation
- Gastrointestinal motility and disorders
- Digestive system and related health
- Drug Transport and Resistance Mechanisms
- Folate and B Vitamins Research
- Pancreatic and Hepatic Oncology Research
- Trace Elements in Health
- Inflammatory Bowel Disease
- Microscopic Colitis
- Kruppel-like factors research
- Helicobacter pylori-related gastroenterology studies
- TGF-β signaling in diseases
- NF-κB Signaling Pathways
Medical University of Vienna
2012-2022
Christian Doppler Laboratory for Thermoelectricity
2011-2014
Iron deficiency is a common cause of reactive thrombocytosis, however, the exact pathways have not been revealed. Here we aimed to study mechanisms behind iron deficiency-induced thrombocytosis. Within few weeks, iron-depleted diet caused in young Sprague-Dawley rats, as reflected by drop hemoglobin, mean corpuscular volume, hepatic content and hepcidin mRNA liver. Thrombocytosis established parallel. Moreover, platelets produced deficient animals displayed higher platelet volume increased...
Background & AimsIrritable bowel syndrome (IBS) and inflammatory diseases result in a substantial reduction quality of life considerable socioeconomic impact. In IBS, diagnosis treatment options are limited, but evidence for involvement the gut microbiome disease pathophysiology is emerging. Here we analyzed prevalence endoscopically visible mucosal biofilms gastrointestinal associated changes composition metabolism.MethodsThe presence was assessed 1426 patients at 2 European...
Abstract HuR is an RNA-binding protein implicated in immune homeostasis and various cancers, including colorectal cancer. binding to AU-rich elements within the 3′ untranslated region of mRNAs encoding oncogenes, growth factors, cytokines leads message stability translation. In this study, we evaluated as a small-molecule target for preventing cancer high-risk groups such those with familial adenomatosis polyposis (FAP) or inflammatory bowel disease (IBD). human specimens, levels cytoplasmic...
Secondary thrombocytosis is a common clinical feature. In patients with cancer, it risk factor for venous thromboembolic events. inflammatory bowel disease (IBD), so far considered marker of active and may contribute to the increased in this population. Observed effects iron therapy on normalization platelet counts led us hypothesize that itself regulate megakaryopoiesis. Here, we want test effect replacement count activity IBD-associated thrombocytosis.We performed randomized,...
Abstract Background Patients with familial adenomatous polyposis (FAP) are at increased risk for the development of colorectal cancer. Surgery and chemoprevention most effective means to prevent cancer development. Thymoquinone (TQ) is considered main compound volatile Nigella sativa seed oil has been reported possess anticarcinogenic properties. In this study we evaluated chemopreventive properties TQ in a mouse model FAP. Methods APC Min mice were fed chow containing 37.5 mg/kg or 375 12...
Disruption of mucosal structure and barrier function contribute to the pathogenesis inflammatory bowel disease (IBD). Efficacy therapy in IBD is based on endoscopic healing, which occurs by a dynamic interplay epithelial cell regeneration, migration differentiation. Both mesalamine (5-ASA) azathioprine (AZTP) promote this process through mechanisms not clearly understood. We examined molecular pathways implicated that were altered 5-ASA AZTP. Paracellular permeability induced mediators was...
Secondary thrombocytosis is a clinical feature of unknown significance. In inflammatory bowel disease (IBD), considered marker active disease; however, iron deficiency itself may trigger platelet generation. this study we tested the effect therapy on counts in patients with IBD-associated anemia.Platelet were analyzed before and after from four prospective trials. Further, changes hemoglobin, transferrin saturation, ferritin, C-reactive protein, leukocyte counts, compared. subgroup...
Mesalamine (5-ASA) is widely used for the treatment of ulcerative colitis, a remitting condition characterized by chronic inflammation colon. Knowledge about molecular and cellular targets 5-ASA limited clear understanding its activity in intestinal homeostasis interference with neoplastic progression lacking. We sought to identify pathways interfered 5-ASA, using CRC cell lines different genetic background. Microarray was performed gene expression profile 5-ASA-treated untreated cells...
P21-activated kinases (PAKs) are multifunctional effectors of Rho GTPases with both kinase and scaffolding activity. Here, we investigated the effects inflammation on PAK1 signaling its role in colitis-driven carcinogenesis. p-PAK1 (Thr423) were assessed by immunohistochemistry, immunofluorescence, Western blot. C57BL6/J wildtype mice treated a single intraperitoneal TNFα injection. Small intestinal organoids from these PAK1-KO cultured TNFα. NF-κB PPARγ analyzed upon overexpression...
Iron deficiency (ID) is globally prevalent, and apart from anemia associated with thrombocytosis. While considered benign, studies linking thrombotic events prior ID suggest otherwise. Herein we used animal models to assess the influence of on tendency. Sprague-Dawley rats were fed control or iron deficient diets ferric carboxymaltose was reverse ID. Thrombosis induced via stenosis inferior vena cava damage right carotid artery using chloride. Thrombi evaluated histologically high frequency...
<b>Background and study aims</b>: The MiroCam is a new video capsule device offering higher frame rate longer battery life-expectancy. We aimed to quantify its clinical impact performed randomized head-to-head comparison with the EndoCapsule respect of complete small-bowel examinations, diagnostic yield in small bowel, transit time. <b>Patients methods</b>: Patients referred for endoscopy because obscure gastrointestinal bleeding, chronic diarrhea, anemia unknown origin were randomly...
Inflammatory bowel disease is a group of conditions with rising incidence caused by genetic and environmental factors including diet. The chelator ethylenediaminetetraacetate (EDTA) widely used the food pharmaceutical industry among numerous other applications, leading to considerable exposure. Numerous safety studies in healthy animals have revealed no relevant toxicity EDTA. Here we show that, presence intestinal inflammation, EDTA surprisingly capable massively exacerbating inflammation...
Iron deficiency is associated with reactive thrombocytosis; however, the mechanisms driving this phenomenon remain unclear. We previously demonstrated that occurs alongside enhanced megakaryopoiesis in iron-deficient rats, without alterations megakaryopoietic growth factors thrombopoietin, interleukin-6, or interleukin-11.The aim of study was to evaluate megakaryocyte differentiation under iron an vitro model and investigate potential genes involved process.Human erythroleukemia...
<h3>Objective</h3> Lynch syndrome is caused by germline mutations in DNA mismatch repair genes leading to microsatellite instability (MSI) and colorectal cancer. Mesalazine, commonly used for the treatment of UC, reduces MSI vitro. Here, we tested natural compounds such activity applied mesalazine thymoquinone a Msh2<sup>loxP/loxP</sup> Villin-Cre mouse model syndrome. <h3>Design</h3> Flow cytometry was quantitation mutation rates at CA<sub>13</sub> human colon cancer (HCT116) cells that had...
Eisenmangel mit und ohne Anämie ist ein häufiges Problem bei Patienten chronisch entzündlichen Darmerkrankungen (CED) kann einer erheblichen Einschränkung der Lebensqualität Leistungsfähigkeit verbunden sein. Die Arbeitsgruppe für CED Österreichischen Gesellschaft Gatroenterologie Hepatologie (ÖGGH) erarbeitete 5 Konsensus-Statements zu folgenden Inhalten: Diagnose von Anämie, Screening auf Eisenmangel, Behandlung Behandlungsziele. klinische Bedeutung intravenösen Eisensubstitution in Bezug...
Individuals with ulcerative colitis are at high risk of developing colitis-associated cancer. 5-Aminosalicylate (5-ASA) protects from cancer by its antiinflammatory activity as well altering cell growth, inducing apoptosis, and reducing replication errors. So far neither 5-ASA's structural specificity nor pharmacophore group have been identified. Here we compared 5-ASA analogs (4-ASA 3-ASA) metabolite N-acetyl-5-ASA (NAc-5-ASA).Superoxide scavenging was analyzed lucigenin-amplified...
Abstract p21-activated kinase 1 (PAK1) is a serine/threonine that overexpressed in colorectal cancer. PAK1 target of mesalamine [5-aminosylicylic acid (5-ASA)], common drug for the treatment ulcerative colitis with prospective chemopreventive properties. Here, we investigated whether deletion impedes tumorigenesis murine intestinal cancer models. Ten-week-old APCmin or APCmin/PAK1−/− mice were monitored 8 weeks, euthanized, and assessed tumor number size. Six- to 8-week-old PAK1−/− wild-type...
Within the interleukin-10 receptor 1 (IL10R1) gene, two common variants are associated with certain diseases: single-nucleotide polymorphism 3 (SNP3), a serine-138 to glycine mutation is in linkage disequilibrium SNP4, glycine-330 arginine mutation, both of which considered loss-of-function alleles. However, molecular consequence G330R unknown. We investigated possible roles on dynamics IL10R1 surface expression and signal transducer activator transduction (STAT) phosphorylation. HeLa cells...
Abstract Oral iron promotes intestinal tumourigenesis in animal models. In humans, expression of transport proteins are altered colorectal cancer. This study examined whether the route therapy alters and tumour growth. Colorectal adenocarcinoma patients with pre-operative deficiency anaemia received oral ferrous sulphate (n = 15), or intravenous ferric carboxymaltose 15). Paired (normal tissues) samples were compared for loading, transporters, proliferation, apoptosis Wnt signalling using...
Microsatellite instability (MSI) occurs in chronically inflamed colorectal tissue and may evolve to colitis-associated cancer. In vitro data suggest that mesalazine (5-ASA) improves MSI.To analyse the changes MSI 156 distal colonic biopsies of 39 patients with ulcerative colitis had been treated within a randomized, double-blind trial comparing 5-ASA E. coli Nissle (EcN).Two collected before after 1 year treatment. testing was performed using panel eight markers, including 3 dinucleotide 5...