A5004159510- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- Platelet Disorders and Treatments
- Acute Lymphoblastic Leukemia research
- Mast cells and histamine
- Glycosylation and Glycoproteins Research
- Histiocytic Disorders and Treatments
- Chronic Myeloid Leukemia Treatments
- Retinoids in leukemia and cellular processes
- Psoriasis: Treatment and Pathogenesis
- NF-κB Signaling Pathways
- RNA Interference and Gene Delivery
- Polyamine Metabolism and Applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Cytomegalovirus and herpesvirus research
- Cholesterol and Lipid Metabolism
Medical University of Graz
2016-2025
Medical University of Vienna
2008-2019
Immunologie, Immunopathologie et Chimie Thérapeutique
2019
Centre National de la Recherche Scientifique
2019
Institut de Biologie Moléculaire et Cellulaire
2019
University Medical Center of the Johannes Gutenberg University Mainz
2019
Johannes Gutenberg University Mainz
2019
Institute of Immunology
1996-2013
University of Vienna
1994-2010
Novartis (Austria)
1998-2000
Several studies have demonstrated that dendritic cells (DC) can be generated in vitro from CD34+ hemopoietic progenitor cells. The growth requirements for these are poorly characterized, however. In particular, undefined serum/plasma components seem to significantly contribute DC development. We report here the cytokine combination granulocyte-macrophage CSF (GM-CSF) plus TNF-alpha and stem cell factor (SCF) commonly used generation of serum/plasma-supplemented medium is, absence serum...
Abstract Langerhans cells (LCs) in epithelia and interstitial dendritic (intDCs) adjacent connective tissues represent two closely related myeloid-derived DC subsets that exert specialized functions the immune system are of clinical relevance for cell therapy. Both arise from monocyte-committed intermediates response to tissue-associated microenvironmental signals; however, molecular mechanisms underlying myeloid subset specification function remain poorly defined. Using microarray...
Transforming growth factor-β1 (TGF-β1) is a fundamental regulator of immune cell development and function. In this study, we investigated the effects TGF-β1 on differentiation human Langerhans cells (LCs) identified Axl as key effector. belongs to TAM (Tyro3, Axl, Mer) receptor tyrosine kinase family, whose members function inhibitors innate inflammatory responses in dendritic are essential prevention lupus-like autoimmunity. We found that expression induced by during LC precursors acquire...
Abstract Background Aronia melanocarpa is a berry rich in polyphenols known for health benefits. However, the bioavailability of has been questioned, and individual taste acceptance fruit with its specific flavor varies. We recently observed substantial differences tolerability aronia juice among healthy females, half individuals tolerating without complaints. Given importance gut microbiome food digestion, we investigated this secondary analysis randomized placebo-controlled parallel...
Expression of CD68 (macrosialin) in the absence surface and lysosomal lineage marker molecules is a characteristic feature T zone-associated plasmacytoid monocytes, which were recently shown to represent precursors dendritic cells (DC). We demonstrate here minor population strongly CD68-positive (CD68bright) blood that lack all analyzed myeloid (CD14-, CD33-, CD13-, CD11b-, CD11c-) (myeloperoxidase, MPO- lysozyme, LZ-) (0.4 +/- 2% total mononuclear cells). These CD68bright, marker-negative...
Mast cells (MC3) belong to the hemopoietic system and arise from precursor cells. Human MC progenitors can be detected in bone marrow as well peripheral blood (pb) are responsive mast cell growth factor SCF, ligand of c-kit tyrosine kinase receptor. However, little is known about subsets that become committed differentiate into mature human MC. In this study, identity circulating progenitor, previously felt a monocyte (Mo) or basophil (Ba), was investigated. For purpose, CD14+ pb monocytes,...
Abstract New technology allows highly sensitive flow cytometric detection and quantitative analysis of intracellular antigens in normal malignant hemopoietic cells. With this technology, the earliest stages myeloid lymphoid differentiation can easily reliably be identified using antibodies directed against (pro‐)myeloperoxidase/MPO, CD22 CD3 antigens, respectively. Particularly for undifferntiated acute myeloblastic leukemia (AML) cells, immunological demonstration MPO or its enzymatically...
ABSTRACT Human cytomegalovirus (CMV) infection initiates in mucosal epithelia and disseminates via leukocytes throughout the body. Langerhans cells (LCs), immature dendritic (DCs) that reside epithelial tissues, are among first to encounter virus may play important roles immune response, as well pathogenesis hosts for viral replication vehicles dissemination. Here, we demonstrate CD34 + progenitor cell-derived LC-type DCs exhibit a differentiation state-dependent susceptibility CMV...
The c-kit proto-oncogene encodes the receptor for a novel hemopoietic cytokine, termed stem cell factor (SCF) or mast growth (MGF) according to its stimulating spectrum. human SCF/MGF is expressed in subset of normal bone marrow progenitor cells, leukemic myeloid and cells. In present study, effects recombinant regulators (IL-1 through -9, granulocyte-macrophage/granulocyte/macrophage-CSF, IFN, TNF) on product expression were analyzed by indirect immunofluorescence, using anti-SCF/MGFR mAb...
The transcription factor aryl hydrocarbon receptor (AhR) represents a promising therapeutic target in allergy and autoimmunity. AhR signaling induced by the newly described ligand VAF347 inhibits allergic lung inflammation as well suppresses pancreatic islet allograft rejection. These effects are likely mediated via alterations dendritic cell (DC) function. Moreover, induces tolerogenic DCs. Langerhans cells (LCs) immediate targets of exogenous ligands at epithelial surfaces; how they...
Human Langerhans cell (LC) precursors populate the epidermis early during prenatal development and thereafter undergo massive proliferation. The prototypic antiproliferative cytokine TGF-β1 is required for LC differentiation from human CD34+ hematopoietic progenitor cells blood monocytes in vitro. Similarly, deficiency results loss vivo. However, immunohistology studies revealed that niches adult basal (germinal) keratinocyte layers lack detectable TGF-β1. Here we demonstrated these express...
Our previous studies demonstrated that TGF-beta 1 is required for efficient in vitro generation of dendritic cells (DC) from CD34+ progenitor under serum-free conditions. Here we show promotes the growth and differentiation DC primarily by protecting viability precursors not enhancing their proliferative response. Addition to TNF-alpha, granulocyte-macrophage CSF, stem cell factor-supplemented cultures had no significant effect on proportions cycling cells. Already at 72 h culture, however,...
Abstract Purpose: The ETV6 gene has been reported to be fused a multitude of partner genes in various hematologic malignancies with 12p13 aberrations. Cytogenetic analysis six cases childhood acute lymphoblastic leukemia revealed novel recurrent t(8;12)(q13;p13), suggesting involvement ETV6. Experimental Design: Fluorescence situ hybridization was used confirm the t(8;12)(q13;p13) and reverse transcription-PCR identify gene. Detailed immunologic characterization done, owing their lineage...
On the outermost edge of body a dense network dendritic cells (DCs), so-called Langerhans (LCs), represents first immune barrier. The establishment and maintenance this epidermal is dependent on cytokine transforming growth factor-β1 (TGF-β1) expressed by keratinocytes (KC) LCs. We recently identified crucial downstream effector TGF-β1, receptor tyrosine kinase Axl. Axl belongs to TAM family, which also includes Tyro3 Mer, activated through vitamin K-dependent ligands Gas6 Protein S. have...
Background: In atopic dermatitis (AD), the skin is colonized by high levels of Staphylo-coccus aureus (SA), while commensal Staphylococcus epidermidis (SE) are reduced. Levels SA correlate with severity disease, transplantation SE into can ameliorate AD. Objectives: It hypothesized that underlying mechanism supported Langerhans cells (LCs) which sense a shift in microbiome and then either continue to perpetuate disease (with SA) or act as anti-inflammatory agents, ameliorating inflamma-tion...