A5016775133- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Analytical Chemistry and Chromatography
- Fungal and yeast genetics research
- Mass Spectrometry Techniques and Applications
- Bone Tissue Engineering Materials
- Polyamine Metabolism and Applications
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- interferon and immune responses
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Immune cells in cancer
- Alzheimer's disease research and treatments
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- Folate and B Vitamins Research
- Fermentation and Sensory Analysis
- Metabolism and Genetic Disorders
- Chromatin Remodeling and Cancer
- Nutritional Studies and Diet
- Microbial metabolism and enzyme function
- Drug Transport and Resistance Mechanisms
Riga Technical University
2021-2025
Innovation (Latvia)
2021-2024
CeMM Research Center for Molecular Medicine
2018-2023
Austrian Academy of Sciences
2018-2023
Biocrates Life Sciences (Austria)
2015-2018
BOKU University
2012-2015
Austrian Centre of Industrial Biotechnology (Austria)
2012-2015
University of Latvia
2009-2012
Abstract Introduction The Alzheimer's Disease Research Summits of 2012 and 2015 incorporated experts from academia, industry, nonprofit organizations to develop new research directions transform our understanding disease (AD) propel the development critically needed therapies. In response their recommendations, big data at multiple levels are being generated integrated study network failures in disease. We used metabolomics as a global biochemical approach identify peripheral metabolic...
As the lipidomics field continues to advance, self-evaluation within community is critical. Here, we performed an interlaboratory comparison exercise for using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The study comprised 31 diverse laboratories, with each laboratory different workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates associated uncertainties...
Recently, quantitative metabolomics identified a panel of 10 plasma lipids that were highly predictive conversion to Alzheimer's disease (AD) in cognitively normal older individuals (n = 28, area under the curve [AUC] 0.92, sensitivity/specificity 90%/90%).Quantitative targeted serum using an identical method as index study.We failed replicate these findings substantially larger study from two independent cohorts-the Baltimore Longitudinal Study Aging ([BLSA], n 93, AUC 0.642, 51.6%/65.7%)...
Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for production of heterologous proteins also serves model organism organelle research. Our current knowledge mainly derives from sophisticated biochemical studies which identified many key utilization enzymes such alcohol oxidase dihydroxyacetone synthase their localization peroxisomes. C1...
Abstract Background Metabolomic processes have been identified as being strongly linked to the development of Alzheimer's disease (AD). Thus, lipid metabolites appear be highly useful diagnostic substrates for diagnosis AD and mild cognitive impairment (MCI) in plasma. Methods We analyzed plasma samples from controls (n = 35), MCI 33), patients 43) using AbsoluteIDQ p180 Kit (Biocrates Life Sciences), which included quantitative analysis 40 acylcarnitines, 21 amino acids, 19 biogenic amines,...
Alzheimer's disease (AD) is a severe and chronic neurodegenerative disorder of the brain. The laboratory diagnosis limited to analysis three biomarkers in cerebrospinal fluid (CSF): amyloid-β42 (Aβ42 ), total tau, phospho-tau-181 (P-tau
Background & AimsIrritable bowel syndrome (IBS) and inflammatory diseases result in a substantial reduction quality of life considerable socioeconomic impact. In IBS, diagnosis treatment options are limited, but evidence for involvement the gut microbiome disease pathophysiology is emerging. Here we analyzed prevalence endoscopically visible mucosal biofilms gastrointestinal associated changes composition metabolism.MethodsThe presence was assessed 1426 patients at 2 European...
Although the host defense mechanisms against SARS-CoV-2 infection are still poorly described, they of central importance in shaping course disease and possible outcome. Metabolomic profiling may complement lacking knowledge molecular underlying clinical manifestations pathogenesis COVID-19.
Environmental microbial triggers shape the development and functionality of immune system. Alveolar macrophages (AMs), tissue-resident lungs, are in constant direct contact with inhaled particles microbes. Such exposures likely impact AM reactivity to subsequent challenges by immunological imprinting mechanisms referred as trained immunity. Here, we investigated whether a ubiquitous compound has potential induce training vivo. We discovered that intranasal exposure ambient amounts...
The intestinal epithelium has a high turnover rate and constantly renews itself through proliferation of crypt cells, which depends on insufficiently characterized signals from the microenvironment. Here, we showed that colonic macrophages were located directly adjacent to epithelial cells in mice, where they metabolically supported cell an mTORC1-dependent manner. Specifically, deletion tuberous sclerosis complex 2 (Tsc2) activated mTORC1 signaling protected against colitis-induced damage...
The Cell Counting Kit-8 (CCK-8) cell viability assay, also known as WST-8, is widely recognized for its nontoxic nature, making it suitable further studies on treated cells. This practice commonly observed in the field of tissue engineering. While live/dead imaging may not readily reveal macroscopic differences, our investigation has uncovered significant intracellular metabolic changes. Notably, we substantial down-regulation metabolites within glycolysis and pentose phosphate pathways....
Abstract Alzheimer’s disease (AD) is the most common neurodegenerative presenting major health and economic challenges that continue to grow. Mechanisms of are poorly understood but significant data point metabolic defects might contribute pathogenesis. The Alzheimer Disease Metabolomics Consortium (ADMC) in partnership with Neuroimaging Initiative (ADNI) creating a comprehensive biochemical database for AD. Using targeted non- metabolomics lipidomics platforms we mapping pathway network...
Abstract Multinucleated giant cells (MGCs) are implicated in many diseases including schistosomiasis, sarcoidosis and arthritis. MGC generation is energy intensive to enforce membrane fusion cytoplasmic expansion. Using receptor activator of nuclear factor kappa-Β ligand (RANKL) induced osteoclastogenesis model formation, here we report RANKL cellular programming requires extracellular arginine. Systemic arginine restriction improves outcome multiple murine arthritis models its removal...
Quantitative metabolic profiling is preceded by dedicated sample preparation protocols. These multistep procedures require detailed optimization and thorough validation. In this work, a uniformly 13 C ‐labeled ( U ) cell extract was used as tool to evaluate the recoveries repeatability precisions of extraction treatment. A homogenous set biological replicates n = 15 samples P ichia pastoris prepared for these fundamental experiments. range less than 30 intracellular metabolites, comprising...
In this work, a fully automated parallel LC column method was established in order to perform orthogonal hydrophilic interaction chromatography (HILIC) and reversed-phase (RPLC) within one analytical run for targeted quantitative mass spectrometric determination of metabolites from central carbon metabolism. way, the throughput could be significantly improved compared previously dual separation work flows involving two separate runs. Two sample aliquots were simultaneously injected onto...
Purpose PEXS was first described in 1917, yet its etiology still needs clarification. An imbalance between oxidants and antioxidants plays a significant role. leads to various ocular complications, including increased risk during cataract surgery due weak zonules, lens dislocation, reduced visual outcomes. Our study investigates whether metabolomics can provide insights into this pathology. Methods The included 183 patients undergoing at Pauls Stradins Clinical University Hospital. 104 did...
Folate metabolism is intricately linked to purine de novo synthesis through the incorporation of folate-derived one-carbon units into scaffold. Here, we investigate chemical and genetic dependencies caused by mutations in folate enzyme MTHFD1 discover a key role for Nudix hydrolase 5 (NUDT5) regulating synthesis. Through knockout development selective NUDT5 degrader, uncover an unprecedented scaffolding rather than enzymatic activity responsible this phenotype. We find that interacts with...
Bone remodelling is a highly dynamic process dependent on the precise coordination of osteoblasts and haematopoietic-cell derived osteoclasts. Changes in core metabolic pathways during osteoclastogenesis, however, are largely unexplored it unknown whether how these processes involved bone homeostasis.