A5030048257- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- CRISPR and Genetic Engineering
- Pharmacological Effects and Toxicity Studies
- Cell death mechanisms and regulation
- Pharmacogenetics and Drug Metabolism
- Trace Elements in Health
- HIV/AIDS drug development and treatment
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Insect Resistance and Genetics
- Animal Genetics and Reproduction
- Electrochemical sensors and biosensors
- Genetically Modified Organisms Research
- Protein Degradation and Inhibitors
- Metabolomics and Mass Spectrometry Studies
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Innovative Microfluidic and Catalytic Techniques Innovation
- interferon and immune responses
- Enzyme Catalysis and Immobilization
- Acute Myeloid Leukemia Research
- Bioinformatics and Genomic Networks
Austrian Academy of Sciences
2014-2021
CeMM Research Center for Molecular Medicine
2015-2021
MLL-fusions represent a large group of leukemia drivers, whose diversity originates from the vast molecular heterogeneity C-terminal fusion partners MLL. While studies selected have revealed critical pathways, unifying mechanisms across all remain poorly understood. We present first comprehensive survey protein-protein interactions seven distantly related MLL-fusion proteins. Functional investigation 128 conserved MLL-fusion-interactors identifies specific role for lysine methyltransferase...
Abstract As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and EFSA guidance on RA food feed from GM plants (EFSA GMO Panel 2011), applicants need perform a molecular characterisation DNA sequences inserted in plant genome. This Technical Note puts together recommendations quality methodology, analysis reporting when sequencing is used plants. In particular, it applies use Sanger next-generation genetic material...
Regulation of cell and tissue homeostasis by programmed death is a fundamental process with wide physiological pathological implications. The advent scalable somatic genetic technologies creates the opportunity to functionally map such essential pathways, thereby identifying potential disease-relevant components. We investigated basis underlying necroptotic performing complementary set loss-of-function gain-of-function screens. To this end, we established FADD-deficient haploid human KBM7...
The interplay between drugs and cell metabolism is a key factor in determining both compound potency toxicity. In particular, how to what extent transmembrane transporters affect drug uptake disposition currently only partially understood. Most transporter proteins belong two protein families: the ATP-Binding Cassette (ABC) family, whose members are often involved xenobiotic efflux resistance, large heterogeneous family of solute carriers (SLCs). We recently argued that SLCs collectively...
Biological matter is organized in functional networks of different natures among which kinase–substrate and protein–protein interactions play an important role. Large public data collections allowed us to compile corpus interaction around human protein kinases. One the most interesting observations analyzing this network that coherence kinase activity relies on substrate primarily not complexes are formed them. Further dissecting two types at level groups (CMGCs, Tyrosine kinases, etc.) we...
Interrogation of cellular metabolism with high-throughput screening approaches can unravel contextual biology and identify cancer-specific metabolic vulnerabilities. To systematically study the consequences distinct perturbations, we assemble a comprehensive drug library (CeMM Library Metabolic Drugs; CLIMET) covering 243 compounds. We, next, characterize it phenotypically in diverse panel myeloid leukemia cell lines primary patient cells. Analysis response profiles reveals that 77 drugs...
Abstract The activity and potency of a drug is inherently affected by the metabolic state its target cell. Solute Carriers (SLCs) represent largest family transmembrane transporters in humans constitute major determinants cellular metabolism. Several SLCs have been shown to be required for uptake individual chemical compounds into systems, but systematic surveys transporter-drug relationships human cells are currently lacking. We performed series genetic screens haploid cell line HAP1 using...
ABSTRACT Regulation of cell and tissue homeostasis by programmed death is a fundamental process with wide physiological pathological implications. The advent scalable somatic genetic technologies creates the opportunity to functionally map these essential pathways, thereby identifying potential disease-relevant components. We investigated basis underlying necroptotic performing complementary set loss- gain-of-function screens. To this end, we established FADD -deficient haploid human KBM7...
Abstract The interplay between drugs and cell metabolism is a key factor in determining both compound potency toxicity. In particular, how to what extent transmembrane transporters affect drug uptake disposition currently only partially understood. Most transporter proteins belong two protein families: the ATP-Binding Cassette (ABC) family, whose members are often involved xenobiotic efflux resistance, large heterogeneous family of Solute carriers (SLCs). We recently argued that SLCs...