A5029916141- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Urinary Tract Infections Management
- Immune Cell Function and Interaction
- Pediatric Urology and Nephrology Studies
- Mitochondrial Function and Pathology
- Protein Degradation and Inhibitors
- interferon and immune responses
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- ATP Synthase and ATPases Research
- Machine Learning in Bioinformatics
- Histone Deacetylase Inhibitors Research
- Bladder and Urothelial Cancer Treatments
- Virus-based gene therapy research
- Urological Disorders and Treatments
- Pesticide Residue Analysis and Safety
- Metabolomics and Mass Spectrometry Studies
- Thyroid Cancer Diagnosis and Treatment
- Gene expression and cancer classification
- Pancreatic and Hepatic Oncology Research
- Genetics and Neurodevelopmental Disorders
- Lung Cancer Diagnosis and Treatment
- Click Chemistry and Applications
- Analytical Methods in Pharmaceuticals
Boston Children's Hospital
2019-2023
Harvard University
2019-2023
Austrian Academy of Sciences
2014-2022
CeMM Research Center for Molecular Medicine
2014-2022
Mass spectrometry (MS) is a valuable tool for plasma proteome profiling and disease biomarker discovery. However, wide-ranging protein concentrations, along with technical biological variabilities, present significant challenges deep reproducible quantitation. Here, we evaluated the qualitative quantitative performance of timsTOF HT Pro 2 mass spectrometers analysis neat (unfractionated) Proteograph-processed across wide range peptide loading masses liquid chromatography (LC) gradients. We...
Article2 November 2022Open Access Source DataTransparent process TMBIM5 is the Ca2+/H+ antiporter of mammalian mitochondria Shane Austin orcid.org/0000-0002-8698-6055 Department Internal Medicine I and Comprehensive Cancer Center, Medical University Vienna, Austria Contribution: Conceptualization, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing - original draft, review & editing Search for more papers by this author Ronald Mekis Biomedical...
Abstract Blood-based approaches to detect early-stage cancer provide an opportunity improve survival rates for lung cancer, the most lethal world-wide. Multiple blood-based detection using molecular analytes derived from individual ‘omics (cell-free DNA, RNA transcripts, proteins, metabolites) have been developed and tested, generally showing significantly lower sensitivity versus late-stage cancer. We hypothesized that approach multiple types of analytes, including broad untargeted coverage...
Abstract Chromosome‐centric Human Proteome Project aims at identifying and characterizing protein products encoded from all human protein‐coding genes. As of early 2017, 19 837 genes have been annotated in the neXtProt database including 2691 missing proteins that never identified by mass spectrometry. Missing may be low abundant many cell types or expressed only a few body such as sperms testis. In this study, we performed expression proteomics two near‐haploid HAP1 KBM‐7 to hunt for...
Recurrent urinary tract infections (UTIs) pose a significant burden on the health care system. Underlying mechanisms predisposing children to UTIs and associated changes in proteome are not well understood. We aimed investigate of subset who have vesicoureteral reflux (VUR) recurrent because their risk developing infection-related renal damage. Improving diagnostic modalities identify UTI factors would significantly alter clinical management with VUR. profiled proteomes 22 VUR patients low...
Abstract Mitochondrial Ca 2+ ions are crucial regulators of bioenergetics, cell death pathways and cytosolic homeostasis. content strictly depends on transporters. In recent decades, the major players responsible for mitochondrial uptake release have been identified, except /H + exchanger (CHE). Originally identified as K exchanger, LETM1 was also considered a candidate CHE. Defining interactome LETM1, we MICS1, only member TMBIM family. Applying cell-based cell-free biochemical assays, here...
Dynamic changes in histone post-translational modifications (PTMs) regulate gene transcription leading to fine-tuning of biological processes such as DNA replication and cell cycle progression. Moreover, specific constitute docking sites for recruitment damage repair proteins mediation subsequent survival. Therefore, understanding monitoring PTMs that can alter proliferation thus lead disease progression are considerable medical interest. In this study, stable isotope labeling with...
Background: For cases where clinical and cytological data from cold thyroid nodules are ambiguous; presurgical proteomic profiling of fine-needle aspirate biopsies in situ can provide additional diagnostics to avoid invasive surgical intervention thyroidectomy benign or non-cancerous tissue. Methods: The biopsy lysate was digested with trypsin, analysed by liquid chromatography mass spectrometry on a linear trap quadrupole Orbitrap Velos. Remaining peptides were separated reversedphase...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is currently the 3rd leading cause of cancer-related deaths in US. Although all-stage 5-year survival rate ~10%, early-stage markedly superior and excess 40%. Hence, early detection PDAC via blood-based liquid biopsies holds promise to reduce morbidity mortality. PrognomiQ’s multi-omics platform performs deep unbiased molecular profiling blood samples detect proteins, metabolites, lipids, mRNA, miRNA, cfDNA fragmentation copy-number, CpG...
Abstract Lung cancer is the leading cause of cancer-related deaths in United States, with estimates 236,740 new cases and 118,830 2022 secondary to disease. Blood-based liquid biopsies hold promise reduce morbidity mortality from lung by enabling early detection downstage disease at diagnosis, theragnostic identification patients most likely be helped or harmed therapy, monitoring therapeutic efficacy, residual PrognomiQ’s multi-omics platform comprehensively profiles proteins, metabolites,...
Spinal cord injury (SCI) evokes profound bladder dysfunction. Current treatments are limited by a lack of molecular data to inform novel therapeutic avenues. Previously, we showed systemic inosine treatment improved function following SCI in rats. Here, applied multi-omics analysis explore alterations the and their sensitivity SCI. Canonical pathways regulated included those associated with protein synthesis, neuroplasticity, wound healing, neurotransmitter degradation. Upstream regulator...
You have accessJournal of UrologyPediatrics: Urinary Tract Infection & Vesicoureteral Reflux (MP64)1 Apr 2019MP64-09 URINE FROM THE PATIENTS WITH VESICOURETERAL REFLUX REVEALS CHANGES IN HOST AND BACTERIAL METABOLISM AFTER URINARY TRACT INFECTION Dijana Vitko*, Kohei Hasegawa, Joseph W. McQuaid, Kylie H. Davis, Maggie R. Leary, Shannon E. DiMartino, Mansbach M. Jonathan, and Richard S. Lee Vitko*Dijana Vitko* More articles by this author , HasegawaKohei Hasegawa McQuaidJoseph McQuaid...
Abstract Infections induce complex host responses linked to antiviral defense, inflammation and tissue damage repair. These processes are increasingly understood involve systemic metabolic reprogramming. We hypothesized that the liver as a central hub may orchestrate many of these changes during infection. Thus, we investigated interplay between metabolism in mouse model chronic viral infection hepatitis. Here show virus-induced type I interferon (IFN-I) modulates wide-spread alterations...