A5100383166- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Asthma and respiratory diseases
- Diet, Metabolism, and Disease
- Sphingolipid Metabolism and Signaling
- Fatty Acid Research and Health
- Neonatal Respiratory Health Research
- Metabolism and Genetic Disorders
- Alzheimer's disease research and treatments
- Nutritional Studies and Diet
- Histone Deacetylase Inhibitors Research
- Alcohol Consumption and Health Effects
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Gut microbiota and health
- Pediatric health and respiratory diseases
- Tryptophan and brain disorders
- Folate and B Vitamins Research
- Vitamin D Research Studies
- Nutrition, Genetics, and Disease
- Bone health and osteoporosis research
- Metabolism, Diabetes, and Cancer
- Cholesterol and Lipid Metabolism
- Mitochondrial Function and Pathology
Steno Diabetes Centers
2018-2025
Amgen (United States)
2020-2025
Copenhagen Prospective Studies on Asthma in Childhood
2021-2024
University of Copenhagen
2021-2024
Gentofte Hospital
2021-2024
University of Surrey
2023-2024
Pusan National University
1990-2024
Korea Research Institute of Bioscience and Biotechnology
2024
King's College London
2015-2023
Ulsan National Institute of Science and Technology
2020-2023
The aim of this study was to (1) replicate previous associations between six blood lipids and Alzheimer's disease (AD) (Proitsi et al 2015) (2) identify novel lipids, clinical AD diagnosis, progression brain atrophy (left/right hippocampus/entorhinal cortex).
Tumors display increased uptake and processing of nutrients to fulfill the demands rapidly proliferating cancer cells. Seminal studies have shown that proto-oncogene MYC promotes metabolic reprogramming by altering glutamine metabolism in How regulates other amino acids is not fully understood. Using high-performance liquid chromatography (HPLC)-tandem mass spectrometry (LC-MS/MS), we found intracellular levels tryptophan metabolites kynurenine pathway. induced expression transporters SLC7A5...
Recently, quantitative metabolomics identified a panel of 10 plasma lipids that were highly predictive conversion to Alzheimer's disease (AD) in cognitively normal older individuals (n = 28, area under the curve [AUC] 0.92, sensitivity/specificity 90%/90%).Quantitative targeted serum using an identical method as index study.We failed replicate these findings substantially larger study from two independent cohorts-the Baltimore Longitudinal Study Aging ([BLSA], n 93, AUC 0.642, 51.6%/65.7%)...
Abstract Introduction Machine learning (ML) may harbor the potential to capture metabolic complexity in Alzheimer Disease (AD). Here we set out test performance of metabolites blood categorize AD when compared CSF biomarkers. Methods This study analyzed samples from 242 cognitively normal (CN) people and 115 with AD‐type dementia utilizing plasma (n = 883). Deep Learning (DL), Extreme Gradient Boosting (XGBoost) Random Forest (RF) were used differentiate CN. These models internally validated...
Lipids such as ceramides and phosphatidylcholines (PC) have been found altered in the plasma of Alzheimer's disease (AD) patients a number discovery studies.For this reason, levels 6 3 PCs, with different fatty acid length saturation levels, were measured from 412 participants (AD n = 205, Control 207) using mass spectrometry coupled ultra-performance liquid chromatography.After this, associations AD status, brain atrophy, age-related effects studied.In participants, cross-sectional analysis...
Abstract Aims/hypothesis Five clusters based on clinical characteristics have been suggested as diabetes subtypes: one autoimmune and four subtypes of type 2 diabetes. In the current study we replicate cross-validate these in three large cohorts using variables readily measured clinic. Methods independent cohorts, total 15,940 individuals were clustered age, BMI, HbA 1c , random or fasting C-peptide, HDL-cholesterol. Clusters cross-validated against original HOMA measures. addition, between...
A critical and as-yet unmet need in Alzheimer's disease (AD) is the discovery of peripheral small molecule biomarkers. Given that brain pathology precedes clinical symptom onset, we set out to test whether metabolites blood associated with as indexed by cerebrospinal fluid (CSF) AD biomarkers.This study analyzed 593 plasma samples selected from European Medical Information Framework for Disease Multimodal Biomarker Discovery study, individuals who were cognitively healthy (n = 242), had mild...
Liver transplantation (LT) improves daily function and ameliorates gut microbial composition. However, the effect of LT on functionality, which can be related to overall patient benefit, is unclear could affect post-LT course. The aims were determine functionality focusing endotoxemia, bile acid (BA), ammonia metabolism, lipidomics. We enrolled outpatient patients with cirrhosis list followed them until 6 months after LT. Microbiota composition (Shannon diversity individual taxa) analysis...
Type 2 diabetes is a multifactorial disease with multiple underlying aetiologies. To address this heterogeneity, investigators of previous study clustered people according to five subtypes. The aim the current investigate etiology these clusters by comparing their molecular signatures. In three independent cohorts, in total 15,940 individuals were based on clinical characteristics. subset, genetic (N = 12,828), metabolomic 2,945), lipidomic 2,593), and proteomic 1,170) data obtained plasma....
Birth by caesarean section is linked to an increased risk of developing asthma, but the underlying mechanisms are unclear.To elucidate link between birth and asthma using newborn metabolomic profiles integrating early-life gut microbiome data cord blood immunology.We investigated influence on liquid chromatography mass spectrometry dried spots from newborns two independent Copenhagen Prospective Studies Asthma in Childhood cohorts, i.e. COPSAC2010 (n=677) COPSAC2000 (n=387). We assessed...
We identify biomarkers for disease progression in three type 2 diabetes cohorts encompassing 2,973 individuals across molecular classes, metabolites, lipids and proteins. Homocitrulline, isoleucine 2-aminoadipic acid, eight triacylglycerol species, lowered sphingomyelin 42:2;2 levels are predictive of faster towards insulin requirement. Of ~1,300 proteins examined two cohorts, GDF15/MIC-1, IL-18Ra, CRELD1, NogoR, FAS, ENPP7 associated with progression, whilst SMAC/DIABLO, SPOCK1 HEMK2...
Pediatric obesity is a progressive, chronic disease that can lead to serious cardiometabolic complications. Here we investigated the peripheral lipidome in 958 children and adolescents with overweight or 373 normal weight, cross-sectional study. We also implemented family-based, personalized program assess effects of management on 186 clinical setting. Using mass spectrometry-based lipidomics, report an increase ceramides, alongside decrease lysophospholipids omega-3 fatty acids metabolism....
We recently showed that Alzheimer's disease patients have lower plasma concentrations of the phosphatidylcholines (PC16:0/20:5; PC16:0/22:6; and PC18:0/22:6) relative to healthy controls. now extend these findings by examining associations between PCs with cognition brain function (measured regional resting state cerebral blood flow; rCBF) in non-demented older individuals. Within Baltimore Longitudinal Study Aging neuroimaging substudy, participants underwent cognitive assessments 15...
Aberrant regulation of histone deacetylase 2 (HDAC2) was reported for gastric cancers. However, responsive cancer genes in disease onset and progression are less understood. HDAC2 expression studied by quantitative RT-PCR Western blotting. The functional consequences knockdown on cell-cycle regulation, programmed cell death, gene target identification investigated flow cytometry, blotting, electron microscopy, anchorage-independent colony formation, migration assay whole-genome microarray....
Abstract Background There is an urgent need to understand the pathways and processes underlying Alzheimer’s disease (AD) for early diagnosis development of effective treatments. This study was aimed investigate dementia using unsupervised lipid, protein gene multi-omics integrative approach. Methods A lipidomics dataset comprising 185 AD patients, 40 mild cognitive impairment (MCI) individuals controls, two proteomics datasets (295 AD, 159 MCI 197 controls) were used weighted co-expression...
Evidence suggests maternal pregnancy dietary intake and nutrition in the early postnatal period to be of importance for newborn child's health. However, studies investigating diet-related metabolites transferred from mother child on disease risk childhood are lacking. We sought investigate influence vertically atopic diseases infections during preschool age.In Danish population-based COPSAC2010 mother-child cohort, information 10 metabolomics profiles dried blood spots (DBS) at age 2-3 days...
Background EGb761, a standardized Ginkgo biloba extract, has antioxidant and antiplatelet aggregation thus might protect against atherosclerosis. However, molecular functional properties of EGb761 its major subcomponents have not been well characterized. We investigated the effect (bilobalide, kaemferol, quercetin) on preventing atherosclerosis in vitro, rat model type 2 diabetes. Methods Results (100 200 mg/kg) or normal saline (control) were administered to Otsuka Long-Evans Tokushima...
Most subjects in osteoporosis clinical trials were women with postmenopausal and while bridging studies (BMD endpoint) provide an expectation that medications will reduce fracture risk men. This real-world study shows direct evidence of reduction among men (36% hip denosumab). Direct for used is very limited. aims to evaluate the effectiveness denosumab reducing risk. included 13,797 aged ≥ 50 years who had initiated Taiwan. Taiwan's National Health Insurance Research Database includes all...
Abstract We randomly selected and partially sequenced cDNA clones from a library of Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower bud cDNAs. Out 1216 expressed sequence tags (ESTs), 904 were unique or nonredundant. Five hundred eighty-eight (48.4%) had homology to functionally defined genes at the peptide level. Only 5 encoded known flower-specific proteins. Among cDNAs with no similarity protein sequences (628), 184 significant nucleotide registered in databases. these...
Non-alcoholic fatty liver disease (NAFLD) is the most common and can lead to multiple complications, including non-alcoholic steatohepatitis (NASH), cirrhosis, hepatocellular carcinoma. The fibrotic characterized by pathological accumulation of extracellular matrix (ECM) proteins. Type VI collagen alpha3 (Col6a3) a biomarker hepatic fibrosis, its cleaved form, endotrophin (ETP), plays critical role in adipose tissue dysfunction, insulin resistance, breast cancer development. Here, we studied...