A5053748185- Genomics and Chromatin Dynamics
- Plant-Microbe Interactions and Immunity
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Plant Molecular Biology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- Plant Virus Research Studies
- Developmental Biology and Gene Regulation
- Plant Stress Responses and Tolerance
- Glutathione Transferases and Polymorphisms
- Epigenetics and DNA Methylation
- Pharmacogenetics and Drug Metabolism
- Congenital heart defects research
- Reproductive biology and impacts on aquatic species
- RNA modifications and cancer
- Invertebrate Immune Response Mechanisms
- Pluripotent Stem Cells Research
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Marine Ecology and Invasive Species
- Genomics, phytochemicals, and oxidative stress
- Plant Disease Resistance and Genetics
Johns Hopkins University
2012-2020
AstraZeneca (Sweden)
2019
University of Baltimore
2012
Rockefeller University
2007-2009
Howard Hughes Medical Institute
2007-2009
Gyeongsang National University
1996-2007
Stem cells can self-renew and generate differentiating daughter cells. It is not known whether these maintain their epigenetic information during asymmetric division. Using a dual-color method to differentially label "old" versus "new" histones in Drosophila male germline stem (GSCs), we show that preexisting canonical H3, but variant H3.3, are selectively segregated the GSC, whereas newly synthesized incorporated DNA replication enriched cell. The histone distribution occurs GSCs...
Glutathione <i>S</i>-transferase (GST) zeta (GSTZ1-1) plays a significant role in the catabolism of phenylalanine and tyrosine, deficiency GSTZ1-1 results accumulation maleylacetoacetate its derivatives maleylacetone (MA) succinylacetone. Induction GST subunits was detected liver <i>Gstz1</i><sup>–/–</sup> mice by Western blotting with specific antisera high-performance liquid chromatography analysis glutathione affinity column-purified proteins. The greatest induction observed members mu...
Calmodulin (CaM) is involved in defense responses plants. In soybean ( Glycine max ), transcription of calmodulin isoform 4 GmCaM4 ) rapidly induced within 30 min after pathogen stimulation, but regulation the gene response to poorly understood. Here, we used yeast one-hybrid system isolate two cDNA clones encoding proteins that bind a 30-nt A/T-rich sequence promoter, region contains repeats conserved homeodomain binding site, ATTA. The proteins, GmZF-HD1 and GmZF-HD2, belong zinc finger...
In many adult stem cell lineages, the continuous production of functional differentiated cells depends on maintenance progenitor in an undifferentiated and proliferative state, as well subsequent commitment to proper terminal differentiation. Drosophila male germline (GSC) lineage, a key differentiation factor, Bag marbles (Bam), is required for transition from spermatogonia differentiating spermatocytes. We show that bam mRNA, but not Bam, present spermatocytes, suggesting regulated...
The Drosophila male germline stem cell (GSC) lineage provides a great model to understand maintenance, proliferation, differentiation and dedifferentiation. Here, we use the GSC systematically analyze transcriptome of discrete but continuously differentiating cysts. We first isolated single cysts at each recognizable stage from wild-type testes, which were subsequently applied for RNA-seq analyses. Our data delineate high-resolution atlas in entire lineage: most dramatic switch occurs early...
Abstract We randomly selected and partially sequenced cDNA clones from a library of Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower bud cDNAs. Out 1216 expressed sequence tags (ESTs), 904 were unique or nonredundant. Five hundred eighty-eight (48.4%) had homology to functionally defined genes at the peptide level. Only 5 encoded known flower-specific proteins. Among cDNAs with no similarity protein sequences (628), 184 significant nucleotide registered in databases. these...
Drosophila spermatogenesis has become a paradigmatic system for the study of mechanisms that regulate adult stem cell maintenance, proliferation and differentiation. The dramatic cellular differentiation process from germline (GSC) to mature sperm is accompanied by dynamic changes in gene expression, which are regulated at transcriptional, post-transcriptional (including translational) post-translational levels. Post-transcriptional regulation been proposed as unique feature germ cells....
Extrinsic cues from the niche are known to regulate adult stem cell self-renewal versus differentiation. Here, we report that an aminopeptidase Slamdance (Sda) acts in Drosophila testicular maintain germline cells (GSCs) and progenitor germ dedifferentiation. Mutations sda lead dramatic deterioration loss. Recombinant Sda has specific activity vitro, vivo function of requires intact domain. is required for accumulation mature DE-cadherin, overexpression DE-cadherin rescues most mutant...
Abstract Direct in vivo reprogramming of cardiac fibroblasts into myocytes is an attractive therapeutic intervention resolving myogenic deterioration. Current transgene-dependent approaches can restore function, but dependence on retroviral delivery and persistent retention transgenic sequences are significant hurdles. Chemical has been established as a legitimate method to generate functional cell types, including those the lineage. Here, we have extended this approach progenitor cells that...