A5034479500- Metabolomics and Mass Spectrometry Studies
- High Altitude and Hypoxia
- Alzheimer's disease research and treatments
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Adipose Tissue and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Diet and metabolism studies
- Organ Transplantation Techniques and Outcomes
- Bioinformatics and Genomic Networks
- Tryptophan and brain disorders
- Genetic Associations and Epidemiology
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Neuroscience of respiration and sleep
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- Cholesterol and Lipid Metabolism
- Neonatal Respiratory Health Research
- Stress Responses and Cortisol
- RNA and protein synthesis mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Gut microbiota and health
- Parasitic infections in humans and animals
- Fatty Acid Research and Health
King's College London
2015-2025
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2024
Wenzhou Medical University
2024
Kunming Medical University
2024
Liaocheng University
2021-2024
Qinghai University
2009-2022
Xuzhou Medical College
2022
Second People’s Hospital of Huai’an
2022
Czech Academy of Sciences, Institute of Psychology
2022
King's College Hospital
2016-2021
There are currently no disease-modifying treatments for Alzheimer's disease (AD), and an understanding of preclinical causal biomarkers to help target pathogenesis in the earliest phases remains elusive. Here, we investigated whether 19 metabolites previously associated with midlife cognition-a predictor AD-translate later clinical risk, using Mendelian randomization (MR) tease out AD-specific relationships. Summary statistics from largest genome-wide association studies (GWASs) AD were used...
Liver transplantation (LT) improves daily function and ameliorates gut microbial composition. However, the effect of LT on functionality, which can be related to overall patient benefit, is unclear could affect post-LT course. The aims were determine functionality focusing endotoxemia, bile acid (BA), ammonia metabolism, lipidomics. We enrolled outpatient patients with cirrhosis list followed them until 6 months after LT. Microbiota composition (Shannon diversity individual taxa) analysis...
Abstract Background There is an urgent need to understand the pathways and processes underlying Alzheimer’s disease (AD) for early diagnosis development of effective treatments. This study was aimed investigate dementia using unsupervised lipid, protein gene multi-omics integrative approach. Methods A lipidomics dataset comprising 185 AD patients, 40 mild cognitive impairment (MCI) individuals controls, two proteomics datasets (295 AD, 159 MCI 197 controls) were used weighted co-expression...
Abstract Introduction This study employed an integrative system and causal inference approach to explore molecular signatures in blood CSF, the amyloid/tau/neurodegeneration [AT(N)] framework, mild cognitive impairment (MCI) conversion Alzheimer's disease (AD), genetic risk for AD. Methods Using European Medical Information Framework (EMIF)‐AD cohort, we measured 696 proteins cerebrospinal fluid ( n = 371), 4001 plasma 972), 611 metabolites 696), genotyped whole‐blood (7,778,465 autosomal...
Introduction: Alzheimer's disease (AD) is a devastating neurological that disproportionately affects women. This study aimed to investigate sex-specific single lipids associated with AD. Methods: Plasma samples from 841 participants, comprising 306 individuals AD, 165 mild cognitive impairment (MCI), and 370 cognitively healthy controls were curated the AddNeuroMed cohort. Lipidomics identified 268 for each sample. We investigated associations lipid modules single-lipids AD probed causality...
The imbalance between neurotoxic and neuroprotective metabolites of the kynurenine pathway has been implicated in pathophysiology major depressive disorder (MDD) adulthood but not fully investigated among adolescents. In this study, we tested association with risk for remission adolescent depression whether abnormalities are sex specific. Kynurenine were measured plasma at baseline IDEA-RiSCo (Identifying Depression Early Adolescence Risk-Stratified Cohort), a longitudinal study adolescents...
Background and aims The shortage of organs for transplantation has led to increased use procured from donors after cardiac death (DCD). effects on the liver remain poorly understood, however. Using livers obtained DCD versus brain (DBD), we aimed understand how ischemia/reperfusion (I/R) injury alters expression pro-inflammatory markers ceramides influences graft leukocyte infiltration. Methods Hepatocyte inflammation, as assessed by ceramide expression, was evaluated in (n = 13) DBD 10)...
For individuals migrating to or residing permanently in high-altitude regions, environmental hypobaric hypoxia is a primary challenge that induces several physiological pathological responses. It well documented human beings adapt via some protective mechanisms, such as erythropoiesis and overproduction of hemoglobin; however, little known on the alterations plasma proteome profiles accommodation hypoxia. In present study, we investigated differential proteomes high altitude natives lowland...
Some studies of high altitude populations argue that stature reduction results from caloric, rather than hypoxic, stress. However, tradeoff models oxygen and glucose metabolism predict in hypoxemia, will be downregulated. We used assumptions two hypotheses: First, hypoxia targets leg segment growth differentially, second, proportions segments partition the impact into hypoxemic energetic components. A group 113 Han Tibetan middle school children at 3100 m aged 8 to 11 were measured for...
Abstract In mammalian cell culture producing therapeutic proteins, one of the important challenges is use several complex raw materials whose compositional variability relatively high and their influences on poorly understood. Under these circumstances, application spectroscopic techniques combined with chemometrics can provide fast, simple, non‐destructive ways to evaluate material quality, leading more consistent performance. this study, a comprehensive data fusion strategy combining...
Abstract Finding specific biomarkers of liver damage in clinical evaluations could increase the pool available organs for transplantation. Lipids are key regulators cell necrosis and hence this study hypothesised that lipid levels be altered suffering severe ischemia. Matched pre- post-transplant biopsies from donation after circulatory death (DCD, n = 36, mean warm ischemia time 21min) brain (DBD, 76, none) were collected. Lipidomic discovery multivariate analysis (MVA) applied. Afterwards,...
Specific ceramides have been identified as risk markers for cardiovascular disease (CVD) years before onset of disease. Treatment with the glucagon-like peptide-1 receptor agonist (GLP-1RA) liraglutide has shown to induce beneficial changes in lipid profile and reduce CVD. Reducing lipotoxic lipids an antidiabetic drug therapy could be a path towards precision medicine approaches treatment complications diabetes. In this post-hoc study, investigation was carried out on effect CVD-risk...
Monoclonal antibodies (mAbs) are currently the dominant class of biopharmaceuticals. Due to high dosage requirements most mAb therapeutics, productivity and low aggregation prevailing criteria during cell line generation process development. Given that light chains (LCs) play an important role in antibody folding assembly, producing lines also manufacture free LCs, we sought investigate whether there was a relationship between LC levels culture media productivity/quality. To this end, series...
Abstract Background Identifying blood-based signatures of brain health and preclinical pathology may offer insights into early disease mechanisms highlight avenues for intervention. Here, we systematically profiled associations between blood metabolites whole-brain volume, hippocampal amyloid-β status among participants Insight 46—the neuroscience sub-study the National Survey Health Development (NSHD). We additionally explored whether key were associated with polygenic risk Alzheimer’s...
Abstract Cerebrospinal fluid (CSF) is a metabolically diverse biofluid and key specimen for exploring biochemical changes in neurodegenerative diseases. Detecting lipid species CSF using mass spectrometry (MS)-based techniques remains challenging because lipids are highly complex structure, their concentrations span over broad dynamic range. This work aimed to develop robust lipidomics metabolomics method based on commonly used two-phase extraction systems from human samples. Prioritizing...
The aim of the present study was to perform a preliminary analysis characterization and diversity Y-chromosome haplotypes/haplogroups in yak Qinghai Province, China. A total 322 male yaks from nine populations belonging three officially recognized breeds (Gaoyuan, Huanhu Datong) were sampled. Animals genotyped using six previously reported Y-SNPs SRY, USP9Y, UTY, AMELY OFD1Y genes four new gene (g.569A>C, g.578A>C, g.608G>T g.653G>C) identified this study. Seven haplotypes (H1-H7) according...
Nivolumab is a therapeutic fully human IgG4 antibody to programmed death 1 (PD-1). In this study, nivolumab biosimilar, which was produced in our laboratory, analyzed and characterized. Sequence variants that contain undesired amino acid sequences may cause concern during biosimilar bioprocess development. We found low levels of sequence were detected the heavy chain by ultra performance liquid chromatography (UPLC) tandem mass spectrometry. It further identified with UPLC-MS/MS IdeS or...
Abstract Purpose Biliary leakage caused by cystobiliary communication (CBC) is a common clinical concern. This study sought to identify predictors of CBC in hepatic cystic echinococcosis (HCE) patients undergoing hydatid liver cyst surgery and establish nomograms predict CBC. Methods A predictive model was established training cohort 310 HCE diagnosed between January 2013 May 2017. Upon revision the records parameters imaging features these patients, lasso regression used optimize feature...
Investigating associations between metabolites and late midlife cognitive function could reveal potential markers mechanisms relevant to early dementia. Here, we systematically explored the metabolic correlates of outcomes measured across seventh decade life, while untangling influencing life course factors. Using levels 1019 profiled by liquid chromatography-mass spectrometry (age 60-64), evaluated relationships in British 1946 Birth Cohort (