A5023890577- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Renal Transplantation Outcomes and Treatments
- Organ Donation and Transplantation
- Liver Disease Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Pediatric Hepatobiliary Diseases and Treatments
- Mast cells and histamine
- Transplantation: Methods and Outcomes
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Cancer Genomics and Diagnostics
- Heme Oxygenase-1 and Carbon Monoxide
- Liver physiology and pathology
- Gallbladder and Bile Duct Disorders
- Inflammasome and immune disorders
- Pancreatic function and diabetes
- Clinical Nutrition and Gastroenterology
- Congenital Diaphragmatic Hernia Studies
- Global Maternal and Child Health
- Respiratory viral infections research
- Genetic and Kidney Cyst Diseases
- Lung Cancer Research Studies
University Health Network
2018-2025
University of Toronto
2018-2025
Hospital for Sick Children
2020-2025
Toronto General Hospital
2018-2024
King Saud Medical City
2024
King Saud University
2024
King Khalid University Hospital
2024
Qassim University
2024
Ministry of Health
2024
Health Net
2024
Abstract Plasma membrane rupture (PMR) in dying cells undergoing pyroptosis or apoptosis requires the cell-surface protein NINJ1 1 . PMR releases pro-inflammatory cytoplasmic molecules, collectively called damage-associated molecular patterns (DAMPs), that activate immune cells. Therefore, inhibiting and may limit inflammation is associated with excessive cell death. Here we describe an anti-NINJ1 monoclonal antibody specifically targets mouse blocks oligomerization of NINJ1, preventing PMR....
•ScRNA-seq revealed additional macrophage diversity in the NDD (healthy) liver.•CD206+ macrophages PSC liver show reduced responsiveness to stimulation.•CK7+ HNF4A+ transitioning hepatocytes are enriched fibrotic lesions comparison PBC and NDD. BackgroundPrimary sclerosing cholangitis (PSC) is an immune-mediated cholestatic disease characterized by bile retention, biliary tree destruction, progressive fibrosis leading end stage transplantation. There unmet need understand cellular...
Abstract Mast cells contribute to the pathogenesis of experimental autoimmune encephalomyelitis, a rodent model human demyelinating disease multiple sclerosis. Yet their site and mode action is unknown. In both diseases, myelin-specific T are initially activated in peripheral lymphoid organs. However, for occur, these must enter immunologically privileged CNS through breach relatively impermeable blood-brain barrier. this study, we demonstrate that dense population resident mast meninges,...
Background. Normothermic ex vivo kidney perfusion (NEVKP) has shown promising results for preservation, assessment, and reconditioning of allografts in preclinical studies. Here, we report the first North American safety feasibility study deceased donor kidneys grafts transplanted following preservation with NEVKP. Methods. Outcomes 13 human that received 1 to 3 h NEVKP after being transported an anoxic hypothermic machine device were compared a matched control group 26 preserved alone....
Mast cells (MCs) exert a significant pathologic influence on disease severity in C57BL/6 (B6) strain-dependent experimental allergic encephalomyelitis (EAE), model of primary progressive multiple sclerosis (MS). However, relapsing-remitting MS, which is modeled SJL mice, the more prevalent form. Given genetically determined heterogeneity numbers and responsiveness MCs from various strains we asked whether these also this clinically relevant MS using SJL-Kit(W/W-v) mice. Similar to...
Background and aims The shortage of organs for transplantation has led to increased use procured from donors after cardiac death (DCD). effects on the liver remain poorly understood, however. Using livers obtained DCD versus brain (DBD), we aimed understand how ischemia/reperfusion (I/R) injury alters expression pro-inflammatory markers ceramides influences graft leukocyte infiltration. Methods Hepatocyte inflammation, as assessed by ceramide expression, was evaluated in (n = 13) DBD 10)...
Liver transplant is a life-saving therapy that can restore quality life for several pediatric liver diseases. However, it not available to all children who need one. Expertise in medical and surgical management heterogeneous, allocation policies are optimally serving children. Technical variant grafts from both living deceased donors underutilized.Several national efforts improve access outcomes have been instituted include adjustments policies, UNOS-sponsored collaborative improvement...
1Pediatric Liver Transplant Program, Children's Hospital of Los Angeles, CA, USA 2USC Institute, Keck Medical Center University Southern California, 3NYU Langone NYU Health, New York, NY,USA 4Ajmera Center, Health Network, Toronto, Canada 5Division General and Thoracic Surgery, The for Sick Children, 6Transplant Regenerative Medicine Correspondence Blayne Amir Sayed, Division 555 Avenue, Ontario, M5G 1×8 Email: [email protected]
ABSTRACT Background & Aims The liver plays a critical role in metabolism and immune function, yet the contributions of its heterogeneous cell types to these processes remain unclear. While most studies focus on adults, pediatric diseases often present differently, underscoring need for age-specific research. Approach Results To better understand cellular drivers childhood diseases, we generated single-cell RNA-seq (scRNA-seq) maps normal used this map examine disease-related populations...
Background: Although living donor liver transplantation (LDLT) is increasingly adopted for pediatric LT, there limited data whether live donation extends benefits to patients from the time of listing. This study investigated LDLT through an intention-to-treat analysis. Methods: Pediatric candidates listed between 2001-2023 at a single Canadian center were categorized as pLDLT (with potential donor) or pDDLT (without donor). The primary endpoint was overall survival secondary involved...
Background. Alcohol-associated liver disease (ALD) is the leading indication for transplantation (LT) in Western world. Although 6 mo of abstinence no longer a criterion patients with ALD, outcomes living donor LT (LDLT) versus deceased (DDLT) are not well established. Methodss. We performed an intention-to-treat analysis to evaluate impact listing and pursuing primary LDLT (pLDLT) compared DDLT (pDDLT). The endpoint was overall survival from date listing, evaluated using Cox regression...
Inflammasomes are nucleated by receptors that become activated upon cellular stresses including ionic dyshomeostasis. Rather than forming in the cytosol, recent evidence suggests inflammasomes at specific sites cell on cytoskeletal polymers and membrane surfaces of organelles. The NLRP3 inflammasome, which is formed loss cytosolic K + , had been proposed to form intermediate filaments as well vesicles along endocytic pathway. To determine necessary requirement either mechanism, we used...
Paired organ exchange can be used to circumvent living donor-recipient ABO incompatibilities. Herein, we present the first case of successful liver paired in North America. This 2-way swap required 4 simultaneous operations: 2 donor hepatectomies and transplants. A nondirected anonymous gift initiated this domino exchange, alleviating an incompatibility other pair. With careful attention ethical logistical issues, is a feasible option expand pool for incompatible pairs.
Objective: Assess the impact of having a living donor on waitlist outcomes and overall survival through an intention-to-treat analysis. Background: Living-donor liver transplantation (LDLT) offers alternative to deceased donation in face organ shortage. An as-treated analysis revealed that undergoing LDLT, compared staying waiting list, is associated with improved survival, even at Model for End-stage Liver Disease-sodium (MELD-Na) score 11. Methods: transplant candidates listed Ajmera...
Screening recommendations for prostate cancer remain controversial, and no specific guidelines exist screening in renal transplant candidates. To examine whether the use of prostate-specific antigen (PSA)-based patients with ESRD affects time to transplantation outcomes, we retrospectively analyzed 3782 male ≥18 years age undergoing primary evaluation during a 10-year period. Patients were grouped by per American Urological Association guidelines: group 1, <55 years; 2, 55-69 3, >69 years. A...
The Model for End-stage Liver Disease (MELD)-based organ allocation system has significantly decreased mortality on the transplant waiting list patients with end-stage liver disease. However, women have remained at a disadvantage respect to access deceased donor (DDLT) even after introduction of MELD score allocation.To determine whether availability living donation in program can offset inequity (LT) women.This cohort study retrospectively analyzed adult listed LT University Health Network...