A5101800288- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Folate and B Vitamins Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Collagen: Extraction and Characterization
- Pneumonia and Respiratory Infections
- Protein Hydrolysis and Bioactive Peptides
- Circular RNAs in diseases
- Renal and related cancers
- Kruppel-like factors research
- Spinal Dysraphism and Malformations
- Hydrogels: synthesis, properties, applications
- Bacterial Infections and Vaccines
- Calcium Carbonate Crystallization and Inhibition
- Inflammasome and immune disorders
- Fetal and Pediatric Neurological Disorders
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Advanced Materials and Mechanics
- Mechanisms of cancer metastasis
- Bone Metabolism and Diseases
- Gestational Diabetes Research and Management
- Cardiomyopathy and Myosin Studies
Yan'an University
2023-2024
China Medical University
2018-2024
Institute of Food Science and Technology
2019-2023
Sheng Jing Hospital
2023
Wageningen University & Research
2022-2023
Chinese Academy of Agricultural Sciences
2019-2022
First Hospital of China Medical University
2020-2022
Hubei University of Science and Technology
2022
Harbin Medical University
2015-2016
Pneumococcal pneumonia has been a significant cause of morbidity and mortality throughout human history. Failing to control pneumococcal resolve ongoing inflammation in host can sepsis, namely pneumosepsis, death ensues. Few theories have suggested an optimally therapeutic option for this infectious disease.
Collagen peptides are widely used in food and medicine industries. Given the significant role of collagen bone structure, considered promising for osteoporosis prevention treatment. To elucidate their effect on osteoporosis, we extracted porcine collagen, digested it into peptides, investigated biological function using osteoblast MC3T3-E1 cells. The structure was relatively stable prepared with different molecular weights (MWs) via hydrolysis two commercially-available enzymes...
Folate metabolism is intricately linked to purine de novo synthesis through the incorporation of folate-derived one-carbon units into scaffold. Here, we investigate chemical and genetic dependencies caused by mutations in folate enzyme MTHFD1 discover a key role for Nudix hydrolase 5 (NUDT5) regulating synthesis. Through knockout development selective NUDT5 degrader, uncover an unprecedented scaffolding rather than enzymatic activity responsible this phenotype. We find that interacts with...
Abstract Impaired autophagy and excessive apoptosis disrupt cellular homeostasis contribute to neural tube defects (NTDs), which are a group of fatal disabling birth caused by the failure closure during early embryonic development. However, regulatory mechanisms underlying NTDs outcomes remain elusive. Here, we report role transcription factor nuclear I-C (NFIC) in maintaining NTDs. We demonstrated that abnormally elevated levels NFIC mouse model can interact with miR-200b promoter, leading...
Epithelial-mesenchymal transition (EMT) is critical for carcinoma invasiveness and metastasis. To investigate the role of membrane-type-2 matrix metalloproteinase (MT2-MMP) in EMT, we generated lentiviral constructs wild-type (WT) an inactive Glu260Ala (E260A) mutant MT2-MMP derived stably transfected HCT116 A549 cell lines. WT-transfected cells appeared mesenchymal-like, whereas with E260A were epithelial-like, as treated MMP inhibitor (GM6001). Expression E-cadherin, β-catenin, zonula...
Purpose Rheumatoid arthritis (RA) is a disease associated with multiple factors. Epigenetics can affect gene expression without altering the DNA sequence. In this study, we aimed to comprehensively analyze epigenetic regulation in RA. Methods Using Gene Expression Omnibus database, identified methylation chip, RNA-sequencing, and miRNA microarray for First, searched methylation, genes, miRNAs RA using differential analysis. Second, determined regulatory networks RA-specific miRNA, m6A...
Abstract Aims Failure of neural tube closure resulting from excessive apoptosis leads to defects (NTDs). NADPH oxidase 4 (NOX4) is a critical mediator cell growth and death, yet its role in NTDs has never been characterized. NOX4 potential target miR‐322, we have previously demonstrated that miR‐322 was involved high glucose‐induced NTDs. In this study, investigated the effect on embryonic neuroepithelium reveal new regulatory mechanism for disrupts neurulation by ameliorating apoptosis....
Conventional synthetic hydrogels are intrinsically soft and brittle, which severely limits the scope of their applications. A variety approaches have been proposed to improve mechanical strength hydrogels. However, a facile ubiquitous strategy prepare with high stiffness is still challenge. Here, we report general highly filled microcomposite performance using an ultrasonic assisted strategy. The microparticles were dispersed in polymer network evenly, resulting homogeneous closely packed...
Abstract Neural tube defects (NTDs) are serious congenital malformations. In this study, we aimed to identify more specific and sensitive maternal serum biomarkers for noninvasive NTD screenings. We collected from 37 pregnant women carrying fetuses with NTDs 38 normal fetuses. Isobaric tags relative absolute quantitation were conducted differential proteomic analysis, an enzyme‐linked immunosorbent assay was used validate the results. then a support vector machine (SVM) classifier establish...
Abstract Spinal bifida aperta (SBA) is a congenital malformation with high incidence. Bone marrow mesenchymal stem cell (BMSC) transplantation has the potential to repair structure of damaged tissues and restore their functions. This an optional treatment that can be used as supplement surgery in SBA. However, application BMSCs limited, neuronal differentiation rate not satisfactory when treating severe Thus, we aimed assess effect neural (NSC)-derived exosomes on BMSC observe therapeutic ex...
Neural tube defects (NTDs) are complex abnormalities associated with gene-environment interactions. The underlying cause has not been determined. Spinal cord tissues from cases NTDs and healthy controls were collected. Methylation patterns between normal individuals compared using 450K Infinium BeadChip Illumina. DNA methylation analysis by pyrosequencing (PyroMark Q96) mRNA protein expression analyzed real-time quantitative PCR Western blotting, respectively. Next-generation Sanger...
The molecular mechanisms of ovarian cancer cell invasion under hypoxia remain unclear. Here we employed a 3D collagen model and chick chorioallantoic membrane (CAM) assay to explore the influence on invasion. Hypoxia (both 1% O 2 CoCl 150 250 µM) induced HO-8910PM in collagenolysis determined by hydroxyproline. Pretreatment with inducible factor-1α inhibitor, YC-1, or MMP GM6001, significantly inhibited degradation proliferation. stimulated both mRNA protein expressions membrane-type 1...
Objective: We investigated the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) supernatants on proliferation, migration, invasion, and apoptosis in glioblastoma (GBM) cell lines RG-2, U251, U87-MG, LN-428, as well their autophagy-mediated through IL-6/JAK2/STAT3 signaling pathway to explore molecular mechanisms. Methods: In this study, LN-428 were treated with 9 mg/ml HUC-MSCs supernatants. Their responses treatment status STAT3 analyzed by multiple experimental approaches...
Endothelial protein C receptor (EPCR), cannabinoid 2 (CBR2), and estrogen α (ERα) play vital roles in osteoblasts proliferation. Also, collagen peptides have proliferation stimulation abilities, di/tri-peptides could be absorbed by the intestine more easily. This study obtained three di/tripeptides with potential abilities of yak bone collagen, namely, MGF, CF, MF, silico screening. Results suggested that these exhibited good absorption, distribution, metabolism, excretion, toxicity (ADMET)...
Abstract Neural tube defects (NTDs) are the most common and severe congenital malformations, which result from failure of neural to close during embryonic development. The etiology NTDs is complex, caused by interactions between genetic environmental factors, but exact mechanisms this disease still not fully understood. We herein employ a Seahorse Bioscience microplate‐based extracellular flux (XF) analyzer determine mitochondrial function quantify respiratory coupling various bioenergetic...
Glycosylation is an important post-translational modification of proteins, contributing to protein function, stability and subcellular localization. Fungal immunomodulatory proteins (FIPs) are a group small with notable activity, some which glycoproteins. In this study, the impact glycosylation on bioactivity biochemical characteristics FIP-nha (from Nectria haematococca) described. Three rFIP-nha glycan mutants (N5A, N39A, N5+39A) were constructed expressed in Pichia pastoris study...
Abstract Background AML is a common hematological malignancy with poor prognosis, the pathogenesis still unclear. lncRNA takes part in occurrence and development of AML. This research aims to explore new differentially expressed lncRNAs their effects on Methods Database‐based bioinformatics analysis was performed screen AML, real‐time PCR used analyze gene expression. Kaplan–Meier survival determine prognostic effect AC026150.8 The cell drug resistance experiment test chemo‐resistance cells....
Aim: Protein vaccines have been the focus of research for vaccine development due to their safety record and facile production. Improving stability proteins is great significance application protein vaccines. Materials & methods: Based on pneumolysin DnaJ Streptococcus pneumoniae, biomineralization was carried out prepare nanoparticles, thermal tested both in vivo vitro. Results: Mineralized nanoparticles were formed successfully these calcium phosphate-encapsulated resistant proteinase K...
Abstract Amniotic fluid-derived mesenchymal stromal cells (AFMSCs) present different features, depending on the isolation timing and culture conditions. The lack of uniform experimental standards hinders comparison results from studies AFMSCs. Moreover, understanding molecular mechanisms that underlie features AFMSCs isolated at embryonic developmental stages might allow obtention more viable highly proliferative through genetic modification. We pregnant rats day (E)12, E15, E18, E21...
Lung adenocarcinoma (LUAD) is a highly invasive and metastatic malignant tumor with high morbidity mortality. This study aimed to construct prognostic signature for LUAD patients based on metastasis-associated genes (MAGs). RNA expression profiles were downloaded from the Cancer Genome Atlas (TCGA) database. RRA method was applied identify differentially expressed MAGs. A total of 192 significantly robust MAGs determined among seven GEO datasets. initially selected through Lasso Cox...