A5049235392- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Heavy Metal Exposure and Toxicity
- Autophagy in Disease and Therapy
- Seed Germination and Physiology
- Gut microbiota and health
- Galectins and Cancer Biology
- Crop Yield and Soil Fertility
- Probiotics and Fermented Foods
- Turfgrass Adaptation and Management
- Plant Disease Resistance and Genetics
- Celiac Disease Research and Management
- Cannabis and Cannabinoid Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glutathione Transferases and Polymorphisms
- Fungal Plant Pathogen Control
- Renin-Angiotensin System Studies
- Saffron Plant Research Studies
- Pasture and Agricultural Systems
- Flavonoids in Medical Research
- Cancer Mechanisms and Therapy
- Ginger and Zingiberaceae research
- Plant-Microbe Interactions and Immunity
Institute of Food Science and Technology
2020-2024
Chinese Academy of Agricultural Sciences
2020-2024
Guizhou University
2024
Ningxia University
2020-2021
Northwest A&F University
2018-2021
Lanzhou University
2009-2016
Xuzhou Medical College
2016
Affiliated Hospital of Xuzhou Medical College
2016
Shanxi Medical University
2009
First Hospital of Shanxi Medical University
2009
Naringin, a major flavonoid in citrus, has potential for preventing atherosclerosis. The presence the colon of large amount naringin after oral intake might affect gut microbiota. We investigated role microbiota remodeling alleviation atherosclerosis by naringin. Naringin significantly alleviated and lowered serum liver cholesterol levels 24.04 28.37% ApoE–/– mice fed with high-fat diet. Nontarget metabolomics showed that modulated hepatic derivatives bile acids. increased excretion acids...
WNK1 and WNK4 are unusual serine/threonine kinases with atypical positioning of the catalytic active-site lysine (WNK: With-No-K[lysine]). Mutations in these WNK kinase genes can cause familial hyperkalemic hypertension (FHHt), an autosomal dominant, hypertensive, disorder, implicating this novel pathway normal regulation BP electrolyte balance. Full-length (WNK1-L) short (WNK1-S) kinase-deficient isoforms previously have been identified. Importantly, WNK1-S is overwhelmingly predominant...
Recent reports have highlighted the relationship between cadmium (Cd) and autophagy, however, whether Cd can activate mitophagy remains enigmatic. This study aims to investigate effects of on its potential mechanism.Mice were intraperitoneally injected with for 3 d. Mitochondrial membrane (MMP), mitophagosomes, LC3-II/LC3-I ratio, PINK1 level mitochondrial mass evaluated indicate mitophagy. To elucidate mechanism, reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine (NAC) or...
The objective of the current study was to explore inhibitory effects quercetin on cadmium-induced autophagy in mouse kidneys. Mice were intraperitoneally injected with cadmium and once daily for 3 days. LC3-II/β-actin ratio used as marker, observed by transmission electron microscopy. Oxidative stress investigated terms reactive oxygen species, total antioxidant capacity, malondialdehyde. Cadmium significantly induced typical autophagosome formation, increased ratio, species level,...
Plant leucine-rich repeat (LRR) receptor-like kinases (RLKs) and LRR proteins (RLPs) comprise a large family of cell surface receptors that play critical roles in signal perception transduction. Both LRR-RLKs LRR-RLPs rely on regulatory to initiate downstream signaling pathways. BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1/SOMATIC EMBRYOGENESIS RECEPTOR 3 (BAK1/SERK3) SUPPRESSOR OF BIR1-1 (SOBIR1) are important extensively studied with distinct functions. Although the mechanism BAK1...
Patulin (PAT) is a mycotoxin frequently detected in moldy fruits and fruit products. This study investigated the protective role of glutathione (GSH), an antioxidant agent, against PAT-induced cytotoxicity its potential mechanisms HEK293 cells. The obtained results showed that addition GSH significantly increased cell viability decreased apoptosis induced by PAT. Additionally, intracellular ROS mitochondrial overproduction, suppressed decline membrane potential, maintained cellular ATP...
Native grasses play an important role in the sustainable development of grassland animal husbandry, land protection, and lawn industry. However, with seed yields often low variable, it is necessary to develop techniques for increasing production these species. From 2007 2012, field trials using nine planting densities (5, 10, 15, 20, 25, 30, 35, 40, 50 plants m −2 ) were conducted arid region China. The effect density on yield its components was investigated a drought‐tolerant grass species,...
The human gut microbiota regulates nutritional metabolism, especially by encoding specific ferulic acid esterases (FAEs) to release functional (FA) from dietary fiber. In our previous study, we observed seven upregulated FAE genes during in vitro fecal slurry fermentation using wheat bran. Here, a 29 kDa (AsFAE) Alistipes shahii of Bacteroides was characterized and identified as the type-A FAE. X-ray structure AsFAE has been determined, revealing unique α-helical domain comprising five...
Glycosylation is an important post-translational modification of proteins, contributing to protein function, stability and subcellular localization. Fungal immunomodulatory proteins (FIPs) are a group small with notable activity, some which glycoproteins. In this study, the impact glycosylation on bioactivity biochemical characteristics FIP-nha (from Nectria haematococca) described. Three rFIP-nha glycan mutants (N5A, N39A, N5+39A) were constructed expressed in Pichia pastoris study...
Fungal immunomodulatory proteins (FIPs) have been investigated for their use as potential natural derived anti-tumor molecules. However, the stability of FIPs is critical preparation and storage. In this study, correlation between thermal protein structural features rFIP-nha, with significant activity, has evaluated. For comprehensive analysis, FIP-nha its homologues FIP-gmi, FIP-fve, LZ-8 were all recombinantly expressed in E. coli. solution, rFIP-nha rFIP-gmi formed tetramers; rFIP-fve...
Understanding how the human gut microbiota contribute to metabolism of dietary carbohydrates is great interest, particularly those with ferulic acid (FA) decorations that have manifold health benefits. Here, we report crystal structure a decameric feruloyl esterase (BtFae) from Bacteroides thetaiotaomicron in complex methyl ferulate (MFA), revealing MFA situated noncatalytic substrate binding pocket adjacent catalytic pocket. Molecular docking and mutagenesis studies further demonstrated...
Gut bacteria-derived enzymes play important roles in the metabolism of dietary fiber through enabling hydrolysis polysaccharides. In this study, we identified and characterized a 29 kDa novel acetyl xylan esterase, BTAxe1, from Bacteroides thetaiotaomicron VPI5482. Then, solved structure BTAxe1 performed rational design. Mutants N65S N65A increased activities toward short-chain (pNPA, pNPB) to near four-fold, gained longer-chain substrate (pNPO). Molecular docking analysis showed that mutant...
To provide a theoretical reference to nitrogen fertilizer management for maize and eco-enviromental protection in the irrigated area of Ningxia, we established critical concentration (Nc) dilution curve under drip-irrigated condition. The nutrition index (NNI) was deduced from Nc model, diagnose feasibility nutrition. Six levels application rates including 0, 90, 180, 270, 360 450 kg·hm-2 were conducted field with variety 'Tianci 19'. During two-year study, NNI model based on leaf dry matter...
Celiac disease (CD) is a prevalent disorder with autoimmune features. Dietary exposure of wheat gluten (including gliadins and glutenins) to the small intestine activates gluten-reactive CD4 + T cells controls development. While human leukocyte antigen (HLA) single most important genetic factor this polygenic disorder, HLA-DQ2 recognition major biological step among patients CD. Gluten epitopes are often rich in Pro share similar primary sequences. Here, we simulated solution structures...