A5022608073- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Pancreatic function and diabetes
- Gut microbiota and health
- Metabolism, Diabetes, and Cancer
- Plant biochemistry and biosynthesis
- Horticultural and Viticultural Research
- Melanoma and MAPK Pathways
- Protein Hydrolysis and Bioactive Peptides
- Cancer Research and Treatments
- Lignin and Wood Chemistry
- Diabetes Treatment and Management
- Probiotics and Fermented Foods
- Food composition and properties
- Sesquiterpenes and Asteraceae Studies
- Biochemical and biochemical processes
- GABA and Rice Research
- Fermentation and Sensory Analysis
- Diet, Metabolism, and Disease
- Streptococcal Infections and Treatments
- Plant-Microbe Interactions and Immunity
Institute of Food Science and Technology
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Center for Life Sciences
2013
Tsinghua University
2013
Zhengzhou University
2011
Background & Aims Accumulating research has addressed the linkage between changes to gut microbiota structure and type 2 diabetes (T2D). Inulin is one of soluble dietary fiber that can alleviate T2D. As a prebiotic, inulin cannot be digested by humans, but rather probiotics. However, whether treatment benefit entire bacteria community remains unknown. In this study, we evaluated differences in composition among diabetic, inulin-treated normal control, control rats. Methods A diabetic rat...
Collagen peptides are widely used in food and medicine industries. Given the significant role of collagen bone structure, considered promising for osteoporosis prevention treatment. To elucidate their effect on osteoporosis, we extracted porcine collagen, digested it into peptides, investigated biological function using osteoblast MC3T3-E1 cells. The structure was relatively stable prepared with different molecular weights (MWs) via hydrolysis two commercially-available enzymes...
Human gut bacteria contribute significantly to human health and several studies have evaluated the effects of dietary fibers on bacterial ecology. However, relationship between different degrees fiber polymerization is unknown. Here, we analyzed three substrates with polymerization, namely carboxymethylcellulose, β-glucans, galactooligosaccharides. To probe in vitro influence degree bacteria, measured pH, air pressure, short-chain fatty acid content fecal fermentation supplemented these...
Varieties of Citrus are commercially important fruits that cultivated worldwide and valued for being highly nutritious having an appealing flavor. Lignification citrus fruit juice sacs is a serious physiological disorder occurs during postharvest storage, which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified isolated candidate MYB transcription factor, CsMYB85, involved in regulation lignin biosynthesis sinensis, has homologs Arabidopsis...
Vanillin is one of the world's most extensively used flavoring agents with high application value. However, yield vanillin biosynthesis remains limited due to low efficiency substrate uptake and inhibitory effect on cell growth caused by vanillin. Here, we screened high-efficiency ferulic acid importer TodX exporters PP_0178 PP_0179 overexpressing genes encoding candidate transporters in a vanillin-producing engineered
Plant leucine-rich repeat (LRR) receptor-like kinases (RLKs) and LRR proteins (RLPs) comprise a large family of cell surface receptors that play critical roles in signal perception transduction. Both LRR-RLKs LRR-RLPs rely on regulatory to initiate downstream signaling pathways. BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1/SOMATIC EMBRYOGENESIS RECEPTOR 3 (BAK1/SERK3) SUPPRESSOR OF BIR1-1 (SOBIR1) are important extensively studied with distinct functions. Although the mechanism BAK1...
Lignin-carbohydrate complexes (LCCs) are emerging as a new and natural product with pharmacological nutraceutical potential. It is uncertain, however, whether LCCs have positive effect on the microbiota of gut based current evidence. Here, LCC extracted from beechwood (BW-LCC) was used substrate for in vitro fermentation. The lignin BW-LCC consisted guaiacyl (G) syringyl (S) units, which mainly linked by β-O-4 bonds. After 24 h fermentation, pH had evidently declined. concentrations acetic...
FtsZ is the core protein for cell division in bacteria that can polymerize into Z-rings and drive cytokinesis. Understanding how maintain correct function of under various environmental stresses crucial novel antibacterial drug discovery. Our previous study revealed S. mutans has higher self-assembly GTPase activity acidic stress, which may be responsible cariogenesis mutans. However, mechanism still unknown. Here, we further reported crystal structure FtsZ, revealing a unique lateral...
Lung cancer is a common disease that associated with poor prognosis. Fungal immunomodulatory protein from Nectria haematococca (FIP-nha) has potential as lung therapeutic; such, illuminating its anti-tumor mechanism expected to facilitate novel treatment options. Here, we showed FIP-nha affects adenocarcinoma growth ex vivo and in vivo. Comparative quantitative proteomics negatively regulates PI3K/Akt signaling induces cell cycle arrest, autophagy, apoptosis. We further demonstrated...
Xylanases (EC 3.2.1.8) are a kind of enzymes degrading xylan to xylooligosaccharides (XOS) and have been widely used in variety industrial applications. Among them, xylanases from thermophilic microorganisms distinct advantages industries that require high temperature conditions. The CoXynA gene, encoding glycoside hydrolase (GH) family 10 xylanase, was identified Caldicellulosiruptor owensensis overexpressed Escherichia coli. Recombinant showed optimal activity at 90 °C with half-life about...
Wheat bran is a cereal rich in dietary fibers that have high levels of ferulic acid, which has prebiotic effects on the intestinal microbiota and host. Herein we explored effect xylooligosaccharide, xylan, whole wheat human gut bacteria screened for potential acid esterase genes. Using vitro fermentation, analyzed air pressure, pH-value, short-chain fatty levels. We also performed 16S rRNA gene metagenomic sequencing. A Venn diagram analysis revealed 80% core operational taxonomic units...
Lung cancer, especially adenocarcinoma, is the second most occurring and highest fatality-causing cancer worldwide. Many natural anticancer compounds, such as sesquiterpene lactones (SLs), show promising properties. Herein, we examined Lactucin, an SL from plant Cichorium intybus, for its cytotoxicity, apoptotic-inducing, cell cycle inhibiting capacity, associated protein expression. We also constructed a biotinylated Lactucin probe to isolate interacting proteins identified them. found that...