Johan Kolmert
A5000092448- Asthma and respiratory diseases
- Eicosanoids and Hypertension Pharmacology
- Metabolomics and Mass Spectrometry Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- IL-33, ST2, and ILC Pathways
- Respiratory and Cough-Related Research
- Allergic Rhinitis and Sensitization
- Mast cells and histamine
- Pediatric health and respiratory diseases
- Pharmacogenetics and Drug Metabolism
- Inflammatory mediators and NSAID effects
- Analytical Chemistry and Chromatography
- Fatty Acid Research and Health
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Inhalation and Respiratory Drug Delivery
- Dermatology and Skin Diseases
- Spectroscopy and Chemometric Analyses
- Respiratory Support and Mechanisms
- Liver Disease Diagnosis and Treatment
- Nutrition, Genetics, and Disease
- Delphi Technique in Research
- Pharmacological Effects and Assays
- Eosinophilic Esophagitis
- Advanced Chemical Sensor Technologies
Karolinska Institutet
2014-2024
Karolinska University Hospital
2021-2024
University of Copenhagen
2023
ENT and Allergy
2020
Umeå University
2014
AstraZeneca (Sweden)
2006-2012
Stockholm University
2006
AstraZeneca (United Kingdom)
2006
As the lipidomics field continues to advance, self-evaluation within community is critical. Here, we performed an interlaboratory comparison exercise for using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The study comprised 31 diverse laboratories, with each laboratory different workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates associated uncertainties...
Monocyte-derived macrophages (MDM) drive the inflammatory response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and they are a major source of eicosanoids in airway inflammation. Here we report that MDM from SARS-CoV-2-infected individuals with mild disease show an transcriptional metabolic imprint lasts for at least 5 months after SARS-CoV-2 infection. convalescent showed downregulation pro-resolving factors increased production pro-inflammatory eicosanoids, particularly...
Chronic obstructive pulmonary disease (COPD) is a heterogeneous and leading cause of mortality morbidity worldwide. The aim this study was to investigate the sex dependency circulating metabolic profiles in COPD. Serum from healthy never-smokers (healthy), smokers with normal lung function (smokers), COPD (COPD; Global Initiative for Obstructive Lung Disease stages I–II/A–B) Karolinska COSMIC cohort (n=116) analysed using our nontargeted liquid chromatography–high resolution mass...
Rationale: New approaches are needed to guide personalized treatment of asthma.Objectives: To test if urinary eicosanoid metabolites can direct asthma phenotyping.Methods: Urinary prostaglandins (PGs), cysteinyl leukotrienes (CysLTs), and isoprostanes were quantified in the U-BIOPRED (Unbiased Biomarkers for Prediction Respiratory Diseases Outcomes) study including 86 adults with mild-to-moderate (MMA), 411 severe (SA), 100 healthy control participants. Validation was performed internally...
Rationale: Mast cells (MCs) play a role in inflammation and both innate adaptive immunity, but their involvement severe asthma (SA) remains undefined. Objectives: We investigated the phenotypic characteristics of U-BIOPRED (Unbiased Biomarkers for Prediction Respiratory Diseases Outcomes) cohort by applying published MC activation signatures to sputum cell transcriptome. Methods: Eighty-four participants with SA, 20 mild/moderate (MMA), 16 healthy without were studied. calculated enrichment...
Background: The anti-IgE monoclonal, omalizumab, is widely used for severe asthma. This study aimed to identify biomarkers that predict clinical improvement during one year of omalizumab treatment. Methods: 1-year, open-label, Study Mechanisms action Omalizumab in Severe Asthma (SoMOSA) involving 216 (GINA step 4/5) uncontrolled atopic asthmatics (≥2 exacerbations previous year) on high-dose inhaled corticosteroids, long-acting β-agonists, ± mOCS. It had two phases: 0-16 weeks, assess early...
Background Approximately 5–10% of patients with asthma have severe disease. A proportion remain symptomatic despite suppression T2-related inflammation but what drives persistent symptoms remains unclear. Eicosanoids exert a functional role in pulmonary inflammation. We explored the relationship between urinary eicosanoids, symptoms, obesity and T2-biomarker status. Methods Urine was sampled during randomized controlled trial assessing corticosteroid optimization using directed care at...
The first step when analyzing multicomponent LC/MS data from complex samples such as biofluid metabolic profiles is to separate the into information and noise via, for example, peak detection. Due nature of this type data, with problems alternating backgrounds differing shapes, can be a very task. This paper presents evaluates two-dimensional detection algorithm based on raw vector-represented data. exploits fact that in high-resolution centroid chromatographic peaks emerge flanked voids...
The clinical importance of prostaglandins and leukotrienes in asthma is well recognized; however, the biochemical role other lipid mediators (often termed oxylipins) regulation airway tone inflammation remains unclear. We therefore developed a workflow to investigate oxylipin physiology pharmacology two vitro models, intact human bronchus guinea pig trachea. Airways were isolated smooth muscle contraction was measured an organ bath following stimulation with either anti-IgE or ovalbumin....
Ximelagatran was developed for the prevention and treatment of thromboembolic conditions. However, in long-term clinical trials with ximelagatran, liver injury marker, alanine aminotransferase (ALT) increased some patients. Analysis plasma samples from 134 patients carried out using proteomic metabolomic platforms, aim finding predictive biomarkers to explain ALT elevation. Analytes that were changed after ximelagatran included 3-hydroxybutyrate, pyruvic acid, CSF1R, Gc-globulin,...
Introduction Asthma is a heterogeneous disease with poorly defined phenotypes. Patients severe asthma often receive multiple treatments including oral corticosteroids (OCS). Treatment may modify the observed metabotype, rendering it challenging to investigate underlying mechanisms. Here, we aimed identify dysregulated metabolic processes in relation severity and medication. Methods Baseline urine was collected prospectively from healthy participants (n=100), patients mild-to-moderate (n=87)...
Proinflammatory bioactive lipid mediators and oxidative stress are increased in coronavirus disease 2019 (COVID-19). The randomized controlled single-blind trial COVID-Omega-F showed that intravenous omega-3 polyunsaturated fatty acids (n-3 PUFA) shifted the plasma signature of COVID-19 towards proresolving precursor levels decreased leukotoxin diols, associated with a beneficial immunodulatory response. present study aimed to determine effects n-3 PUFA on urinary oxylipidome COVID-19. From...
Rationale: Children with preschool wheezing or school-age asthma are reported to have airway microbial imbalances. Objectives: To identify clusters in children using oropharyngeal microbiota profiles. Methods: Oropharyngeal swabs from the U-BIOPRED (Unbiased Biomarkers for Prediction of Respiratory Disease Outcomes) pediatric cohort were characterized 16S ribosomal RNA gene sequencing, and unsupervised hierarchical clustering was performed on Bray-Curtis β-diversity. Enrichment scores...
The eicosanoids are a family of lipid mediators pain and inflammation involved in multiple pathologies, including asthma, hypertension, cancer, atherosclerosis, neurodegenerative diseases. These signaling act locally, but rapidly metabolized transported to the systemic circulation as mixture primary secondary metabolites. Accordingly, urine has become useful readily accessible biofluid for monitoring endogenous synthesis these molecules. Herein, we present validation rapid, repeatable,...
Asthma phenotyping requires novel biomarker discovery.To identify plasma biomarkers associated with asthma phenotypes by application of a new proteomic panel to samples from two well-characterised cohorts severe (SA) and mild-to-moderate (MMA) asthmatics, COPD subjects healthy controls (HCs).An antibody-based array targeting 177 proteins predominantly involved in pathways relevant inflammation, lipid metabolism, signal transduction extracellular matrix was applied 525 asthmatics HCs the...
Oxidative stress is believed to be a major driver of inflammation in smoking asthmatics. The U-BIOPRED project recruited cohort Severe Asthma smokers/ex-smokers (SAs/ex) and non-smokers (SAn) with extensive clinical biomarker information enabling characterization these subjects. We investigated oxidative severe asthma subjects by analysing urinary 8-iso-PGF2α the mRNA-expression main pro-oxidant (NOX2; NOSs) anti-oxidant (SODs; CAT; GPX1) enzymes airways SAs/ex SAn. All were further divided...
This paper addresses the possibility of mathematically partition and process urine 1H-NMR spectra to enhance efficiency subsequent multivariate data analysis in context metabolic profiling a toxicity study. We show that by processing NMR with peak alignment using reduced set mapping (PARS) algorithm use sparse representation results information contained original being preserved retained resolution but free problem shifts. can now describe method for differential expression prior knowledge,...
Patients with systemic mastocytosis (SM) have clinical signs of mast cell (MC) activation and increased levels MC mediators. It is unclear whether the mediator are caused by numbers tissue MCs, or these cells in affected individuals a hyperactive phenotype.To determine reactivity skin airways to directly acting mediators indirectly secretagogues subjects SM.Skin morphine histamine, airway responsiveness mannitol methacholine, was assessed 15 patients SM, 11 allergic asthma (A) 13 healthy...
Scope The fatty acid composition of plasma lipids, which is associated with biomarkers and risk non‐communicable diseases, regulated by dietary polyunsaturated acids (PUFAs) variants desaturase ( FADS ). We investigated the interactions between PUFAs FADS1 rs174550 variant. Methods results Participants n = 118), homozygous for variant (TT CC) followed a high alpha‐linolenic (ALA, 5 percent energy (E‐%)) or linoleic (LA, 10 E‐%) diet during an 8‐week randomized controlled intervention. Fatty...