A5051049041- Mast cells and histamine
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Proteoglycans and glycosaminoglycans research
- Urticaria and Related Conditions
- Carbohydrate Chemistry and Synthesis
- Polyamine Metabolism and Applications
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Eosinophilic Disorders and Syndromes
- melanin and skin pigmentation
- Peroxisome Proliferator-Activated Receptors
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Milk Quality and Mastitis in Dairy Cows
- Synthesis and Biological Evaluation
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Cholesterol and Lipid Metabolism
- Microbial infections and disease research
- Platelet Disorders and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Probiotics and Fermented Foods
Karolinska Institutet
2018-2024
Karolinska University Hospital
2018-2024
Uppsala University
2009-2017
Swedish University of Agricultural Sciences
2004-2015
Bacterial infection with the severe complication of sepsis is a frequent and serious condition, being major cause death worldwide. To cope plethora occurring bacterial infections there therefore an urgent need to identify molecular mechanisms operating during host response, in order both potential targets for therapeutic intervention biomarkers disease. Here we addressed this issue by studying global gene expression uteri from female dogs suffering spontaneously uterine infection.The...
Background: Epithelial cytokines, including IL-33 and Thymic stromal lymphopoietin (TSLP), have attracted interest because of their roles in chronic allergic inflammation-related conditions such as asthma. Mast cells are one the major targets IL-33, to which they respond by secreting cytokines. Most studies performed thus far investigated acute effects on mast cells. In current study, we how vs. prolonged exposure TSLP affects mediator synthesis IgE-mediated activation. Methods: Human lung...
Background Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population cells, with distinct subpopulations identified. However, systematic and comprehensive cell-surface markers to study cell heterogeneity yet be performed. Methods Human were obtained from lobectomies, the expression 332 was analyzed using flow cytometry LEGENDScreen™ kit. Markers exhibited high variance selected for additional analyses reveal...
Serglycin (SG) proteoglycan consists of a small core protein to which glycosaminoglycans chondroitin sulfate or heparin type are attached. SG is crucial for maintaining mast cell (MC) granule homeostasis through promoting the storage various basic constituents, where degree sulfate/heparin sulfation essential optimal functionality. However, regulation expression and sulfotransferases during MC differentiation activation poorly understood. Here we addressed these issues show that protein,...
Background: Mast cells are tissue-resident inflammatory defined by their high granularity and surface expression of the high-affinity IgE receptor, FcεRI, CD117/KIT, receptor for stem cell factor (SCF). There is a considerable heterogeneity among mast cells, both phenotypically functionally. Human generally divided into two main subtypes based on protease content; mucosa-associated MCT (tryptase positive chymase negative cell) connective tissue associated-residing MCTC cell). lung exhibit in...
BackgroundSystemic mastocytosis (SM) is a haematological disease characterised by organ infiltration neoplastic mast cells. Almost all SM patients have mutation in the gene encoding tyrosine kinase receptor KIT causing D816V substitution and autoactivation of receptor. Mast cells CD34+ haematopoietic progenitors can carry mutation; however, which progenitor cell subset arises unknown. We aimed to investigate distribution single stem cells.MethodsFluorescence-activated single-cell index...
Numerous inflammatory skin disorders display a high prevalence of itch. The Mas-related G protein coupled receptor X2 (MRGPRX2) has been shown to modulate itch by inducing non-IgE-mediated mast cell degranulation and the release endogenous inducers pruritus. Various substances collectively known as basic secretagogues, which include peptides certain drugs, can trigger MRGPRX2 thereby induce pseudo-allergic reactions characterized histamine protease well inflammation. Here, we investigated...
Mast cells (MCs) are strongly implicated in immunity toward bacterial infection, but the molecular mechanisms by which MCs contribute to host response only partially understood. We addressed this issue examining direct effects of a Gram-positive pathogen, Streptococcus equi, on bone marrow-derived (BMMCs). Ultrastructural analysis revealed extensive formation dilated rough endoplasmic reticulum indicating strong induction protein synthesis. However, BMMCs did not show signs degranulation,...
Abstract Caspase-3 is a main executioner of apoptotic cell death. The general notion that, in viable cells, caspase-3 found as cytosolic inactive proenzyme and that activation largely confined to processes associated with In this study, we challenge by showing enzymatically active stored mast cells. was undetectable the cytosol but recovered subcellular fractions containing secretory granule-localized proteases. Moreover, rapidly released into compartment after permeabilization granules....
Here we show that mast cells contain dopamine and cell activation causes depletion, indicating its presence within secretory granules. Dopamine storage increased during maturation from bone marrow precursors, was dependent on the of serglycin. Moreover, expression tyrosine hydroxylase, key enzyme in biosynthesis, induced maturation; histidine decarboxylase tryptophan hydroxylase 1 were also induced. Mast caused a robust induction decarboxylase, but no stimulation or expression. The present...
Staphylococcus aureus is a major pathogen that can cause broad spectrum of serious infections including skin infections, pneumonia and sepsis. Peritoneal mast cells have been implicated in the host response towards various bacterial insults to provide mechanistic insight into role intraperitoneal infection we here studied global effects S. on cell gene expression. After co-culture peritoneal with live found by array analysis they up-regulate number genes. Many these corresponded...
SUCNR1 is a sensor of extracellular succinate, Krebs cycle intermediate generated in excess during oxidative stress and has been linked to metabolic regulation inflammation. While mast cells express SUCNR1, its role cell reactivity allergic conditions such as asthma remains be elucidated.Cord blood-derived human line LAD-2 challenged by ligands were analyzed for the activation mediator release. Effects on cell-dependent bronchoconstriction assessed guinea pig trachea isolated small bronchi...
This is the first report describing cloning and characterization of sterol carrier protein-2 (SCP-2) from plants. Arabidopsis thaliana SCP-2 (AtSCP-2) consists 123 amino acids with a molecular mass 13.6 kDa. AtSCP-2 shows 35% identity 56% similarity to human SCP-2-like domain present in D-bifunctional protein (DBP) 30% 54% encoded by SCP-X. The presented structural models apo-AtSCP-2 ligand-bound conformation reveal remarkable two structurally known SCP-2s, DBP rabbit SCP-2, correspondingly....
The Arabidopsis thaliana sterol carrier protein-2 (AtSCP2) is a small, basic and peroxisomal protein that in vitro enhances the transfer of lipids between membranes. AtSCP2 all other plant SCP-2 have been identified are single-domain polypeptides, whereas many eukaryotes domains expressed terminus multidomain polypeptides. transcript analysed tissues developmental stages, with highest levels floral maturing seeds. expression highly correlated multifunctional (MFP2) involved β-oxidation. A....
<b><i>Background:</i></b> Many of the functions attributed to mast cells depend on various pro-inflammatory mediators that are secreted upon cell activation. These include a panel cell-specific proteases. In addition, recent studies have indicated murine also express granzyme D, protease previously thought be confined cytotoxic lymphocytes. Here, we address human relevance latter findings by investigating whether H, may represent functional counterpart D....
Mastitis is a serious medical condition of dairy cattle. Here we evaluated whether the degree virulence mastitis pathogens in mouse model can be linked inflammatory response that they provoke. Clinical isolates Staphylococcus aureus (S. aureus) (strain 556 and 392) E. coli (676 127), laboratory control strains (8325-4 MG1655 (E. coli)), were injected i.p. into mice, followed by assessment clinical scores parameters. As judged scoring, 127 exhibited largest among strains. All bacterial...