A5024423933- Receptor Mechanisms and Signaling
- Dermatology and Skin Diseases
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Urticaria and Related Conditions
- Biochemical Analysis and Sensing Techniques
- Ion Channels and Receptors
- Mast cells and histamine
- Neuroscience and Neuropharmacology Research
- Asthma and respiratory diseases
- Regulation of Appetite and Obesity
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine Tumor Research Advances
- Neurobiology and Insect Physiology Research
- Adipose Tissue and Metabolism
- Food Allergy and Anaphylaxis Research
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Exercise and Physiological Responses
- Machine Learning in Bioinformatics
- Mass Spectrometry Techniques and Applications
- Photoreceptor and optogenetics research
- melanin and skin pigmentation
Uppsala University
2016-2025
Society for Neuroscience
2017
Kyoto University
2009
Swedish University of Agricultural Sciences
2003
The superfamily of G-protein-coupled receptors (GPCRs) is very diverse in structure and function its members are among the most pursued targets for drug development. We identified more than 800 human GPCR sequences simultaneously analyzed 342 unique functional nonolfactory with phylogenetic analyses. Our results show, high bootstrap support, five main families, named glutamate, rhodopsin, adhesion, frizzled/taste2, secretin, forming GRAFS classification system. rhodopsin family largest forms...
We report seven new members of the superfamily human G protein‐coupled receptors (GPCRs) found by searches in genome databases, termed GPR100, GPR119, GPR120, GPR135, GPR136, GPR141, and GPR142. also 16 orthologues these mouse, rat, fugu (pufferfish) zebrafish. Phylogenetic analysis shows that are additional family rhodopsin‐type GPCRs. GPR100 similarity with orphan receptor SALPR. Remarkably, other do not have any close relative among known rhodopsin‐like Most highly conserved through...
G protein-coupled receptors (GPCRs) are one of the largest families proteins, and here we scan recently sequenced chicken genome for GPCRs. We use a homology-based approach, utilizing comparisons with all human GPCRs, to detect verify GPCRs from translated genomic alignments Genscan predictions. present 557 manually curated sequences genome, which 455 were previously not annotated. More than 60% gene predictions ortholog needed curation, drastically changed average percentage identity...
The Adhesion G-protein–coupled receptors (GPCRs) are the most complex gene family among GPCRs with large genomic size, multiple introns, and a fascinating flora of functional domains, though evolutionary origin this has been obscure. Here we studied evolution all class B (7tm2)–related genes, including Adhesion, Secretin, Methuselah families focus on nine genomes. We found that cnidarian genome Nematostella vectensis remarkably rich set broad repertoire N-terminal domains although did not...
Abstract Itch is a sensation that promotes the desire to scratch, which can be evoked by mechanical and chemical stimuli. In spinal cord, neurons expressing gastrin-releasing peptide receptor (GRPR) have been identified as specific mediators of itch. However, our understanding GRPR population in thus how these exercise their functions, limited. For this purpose, we constructed Cre line designed target (Grpr-Cre). Our analysis revealed Grpr-Cre cells cord are predominantly excitatory...
Abstract The ventral tegmental area (VTA) and substantia nigra pars compacta (SNc) of the midbrain are associated with Parkinson’s disease (PD), schizophrenia, mood disorders addiction. Based on recently unraveled heterogeneity within VTA SNc, where glutamate, GABA co-releasing neurons have been found to co-exist classical dopamine neurons, there is a compelling need for identification gene expression patterns that represent this value development human therapies. Here, several unique were...
Acute pain is an unpleasant experience caused by noxious stimuli. How the spinal neural circuits attribute differences in quality of information remains unknown. By means genetic capturing, activity manipulation and single-cell RNA sequencing, we identified distinct ensembles adult mouse cord encoding mechanical heat pain. Reactivation or silencing these potentiated stopped, respectively, paw shaking, lifting licking within but not across stimuli modalities. Within ensembles, polymodal Gal+...
We report eight novel members of the superfamily human G protein‐coupled receptors (GPCRs) found by searches in genome databases, termed GPR97, GPR110, GPR111, GPR112, GPR113, GPR114, GPR115 and GPR116. Phylogenetic analysis shows that these are additional a family GPCRs with long N‐termini, previously EGF‐7TM, LNB‐7TM, B2 or LN‐7TM. Five form their own phylogenetic cluster, while three others cluster reported HE6 GPR56 (TM7XN1). All have GPS domain N‐terminus Ser/Thr‐rich regions forming...
Seven alleles of the chicken melanocortin (MC) 1 receptor were cloned into expression vectors, expressed in mammalian cells and pharmacologically characterized. Four clones e +R , +B&D wh /e y E Rfayoumi gave receptors to which stimulating hormone (α‐MSH) NDP‐MSH bound with similar IC 50 values responded α‐MSH by increasing intracellular cAMP levels a dose‐dependent manner. Three cMC1 receptors; b R did not show any specific binding radioligand, but found be constitutively active assay....
The rainbow trout (Oncorhynchus mykiss) is one of the most widely used fish species in aquaculture and physiological research. In present paper, we report first cloning, 3D (three-dimensional) modelling, pharmacological characterization tissue distribution two melanocortin (MC) receptors trout. Phylogenetic analysis indicates that these are orthologues human MC4 MC5 receptors. We created molecular models their counterparts. These suggest greater divergence between than analyses demonstrated...
Background: EphB receptors and their ephrin-B ligands play an important role in nervous system development, as well synapse formation plasticity the adult brain. Recent studies show that intrathecal treatment with EphB-receptor activator ephrinB2-Fc induced thermal hyperalgesia mechanical allodynia rat, indicating ephrin-B2 small dorsal root ganglia (DRG) neurons spinal cord modulate pain processing. To examine of peripheral pathways, we deleted Nav1.8+ nociceptive sensory Cre-loxP system....
Primary afferents are known to use glutamate as their principal fast neurotransmitter. However, it has become increasingly clear that peptides have an influential role in both mediating and modulating sensory transmission. Here we describe the transmission accounting for different acute pain states itch transmitted via transient receptor potential cation channel subfamily V member 1 (TRPV1) population by either ablating Trpv1–Cre-expressing neurons or inducing vesicular transporter 2...
Summary VGLUT2 is the key mediator of nociception in primary afferents, demonstrated by general effect on pain and itch a mouse line lacking all DRG neurons. Glutamate an essential transmitter pathways. However, its broad usage central peripheral nervous system prevents us from designing efficient glutamate-based therapies without causing harmful side effects. The discovery vesicular glutamate transporters (VGLUT1-3) has been crucial step describing specific glutamatergic neuronal...
Itch is a somatosensory modality that serves to alert an organism harmful elements removable by scratching, such as parasites and chemical irritants. Recently, ablation or silencing of neuropeptide Y (NPY)-expressing spinal interneurons was reported selectively enhance mechanical itch, whereas itch unaffected. We examined the effect activating NPY/Y1 receptor system on scratch behavior in mice. found intrathecal administration Y1 agonist [Leu31,Pro34]-NPY (LP-NPY) attenuated induced...
A major challenge in neuroscience is to resolve the connection between gene functionality, neuronal circuits, and behavior. Most, if not all, circuits of adult brain contain a glutamatergic component, nature which has been difficult assess because vast cellular abundance glutamate. In this study, we wanted determine role restricted subpopulation neurons within forebrain, Vglut2 -expressing neurons, circuitry higher function. expression was selectively deleted cortex, hippocampus, amygdala...
Ablating or functionally compromising sets of sensory neurons has provided important insights into peripheral modality-specific wiring in the somatosensory system. Inflammatory hyperalgesia, cold pain, and noxious mechanosensation have all been shown to depend upon Na v 1.8-positive neurons. The release fast-acting neurotransmitters, such as glutamate, more slowly released neuropeptides, substance P (SP), contribute diversified responses external stimuli. Here we show that deleting Vglut2...
Cell to cell communication is critical for all multicellular organisms, and single-cell sequencing facilitates the construction of full connectivity graphs between types in tissues. Such complex data structures demand novel analysis methods tools exploratory analysis.We propose a method predict putative ligand-receptor interactions from RNA-sequencing data. This achieved by inferring incorporating multi-directional graph, thereby enabling contextual analysis. We demonstrate that our approach...
The transient receptor potential cation channel subfamily V member 1 (TRPV1) is known as a thermosensor and integrator of inflammation-induced hyperalgesia. TRPV1 expressed in subpopulation primary afferent neurons that express several different neurotransmitters. role the development hyperalgesia established, but neurotransmitter glutamate, used partially by same neuronal population thus probably mediating response, still under investigation. We have Trpv1-Cre mouse line which we either...
Numerous inflammatory skin disorders display a high prevalence of itch. The Mas-related G protein coupled receptor X2 (MRGPRX2) has been shown to modulate itch by inducing non-IgE-mediated mast cell degranulation and the release endogenous inducers pruritus. Various substances collectively known as basic secretagogues, which include peptides certain drugs, can trigger MRGPRX2 thereby induce pseudo-allergic reactions characterized histamine protease well inflammation. Here, we investigated...