A5019262940- Hormonal Regulation and Hypertension
- Pharmacogenetics and Drug Metabolism
- Adrenal and Paraganglionic Tumors
- Cancer, Hypoxia, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- Medical Imaging Techniques and Applications
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Eicosanoids and Hypertension Pharmacology
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Sexual Differentiation and Disorders
- Lanthanide and Transition Metal Complexes
- Adipose Tissue and Metabolism
- Neuropeptides and Animal Physiology
- Vitamin K Research Studies
- Chronic Kidney Disease and Diabetes
- Migraine and Headache Studies
- Protease and Inhibitor Mechanisms
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- Parathyroid Disorders and Treatments
- Prostate Cancer Treatment and Research
- Radiomics and Machine Learning in Medical Imaging
Olink Bioscience (Sweden)
2018-2021
Uppsala University
2000-2018
Healthcare in Sweden
2012
General Electric (Spain)
2007-2011
Uppsala University Hospital
2005-2006
Species differences occur in the brain concentrations of drugs, but reasons for these are not yet apparent. This study was designed to compare uptake three radiolabeled P-glycoprotein (P-gp) substrates across species using positron emission tomography. Brain and brain-to-plasma ratios were compared; [<sup>11</sup>C]verapamil rats, guinea pigs, monkeys; [<sup>11</sup>C](<i>S</i>)-(2-methoxy-5-(5-trifluoromethyltetrazol-1-yl)-phenylmethylamino)-2(<i>S</i>)-phenylpiperidine (GR205171) monkeys,...
Effective biomarkers for multiple sclerosis diagnosis, assessment of prognosis, and treatment responses, in particular those measurable blood, are largely lacking. We have investigated a broad set protein cerebrospinal fluid (CSF) plasma using highly sensitive proteomic immunoassay. Cases from two independent cohorts were compared with healthy controls patients other neurological diseases. identified replicated 10 proteins including IL-12B, CD5, MIP-1a, CXCL9 which had combined diagnostic...
Adrenal incidentalomas are common findings necessitating extensive laboratory work-up and repetitive radiological examinations. Positron emission tomography (PET) using (11)C-labeled metomidate (MTO) has previously been described as a tool for specific adrenocortical imaging.We evaluated 212 MTO-PET examinations in 173 patients to identify its role the management of adrenal tumors.Seventy-five histopathological from 73 were retrospectively analyzed.All performed at referral center.Patients...
Shrunken pore syndrome (SPS), originally defined by cystatin C-based estimated glomerular filtration rate (eGFRcystatin C) being less than 60% of creatinine-based (eGFRcreatinine) in the absence extrarenal influences on plasma levels C or creatinine, is associated with a high increase mortality, even reduced (GFR). The objective present study was to determine whether proteome patients SPS shows differences from that normal measured GFR (mGFR) without SPS.Four patient cohorts were included: 1...
A major challenge in neuroscience is to resolve the connection between gene functionality, neuronal circuits, and behavior. Most, if not all, circuits of adult brain contain a glutamatergic component, nature which has been difficult assess because vast cellular abundance glutamate. In this study, we wanted determine role restricted subpopulation neurons within forebrain, Vglut2 -expressing neurons, circuitry higher function. expression was selectively deleted cortex, hippocampus, amygdala...
3-Methylsulfonyl-2,2′-bis(4-chlorophenyl)-1,1′-dichloroethene (MeSO2-DDE) is a potent, tissue-specific toxicant that induces necrosis of the adrenal zona fasciculata following local CYP11B1-catalyzed activation to reactive intermediate in mice. Autoradiography was used examine binding MeSO2-[14C]DDE and adrenocorticolytic drug 2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichlorethane; (o,p′-[14C]DDD, Mitotane, Lysodren) human tissue slice culture. Both compounds gave rise selective one sample...
Proteomics studies are important for the discovery of new biomarkers as clinical tools diagnosis and disease monitoring. However, preanalytical variations caused by differences in sample handling protocol pose challenges assessing biomarker reliability comparability between studies. The purpose this study was to examine effects delayed centrifuging on measured protein levels plasma cerebrospinal fluid (CSF). Blood from healthy individuals patients with multiple sclerosis along CSF suspected...
The persistent adrenocorticolytic DDT metabolite 3-methylsulfonyl-DDE (MeSO(2)-DDE) was originally identified in Baltic grey seals, a population suffering from adrenocortical hyperplasia. In mice, MeSO(2)-DDE induces mitochondrial degeneration and cellular necrosis the adrenal zona fasciculata. this study, we used precision-cut tissue slice culture to examine local CYP11B1-catalyzed irreversible binding of murine cortex. We also examined effects on steroid hormone secretion, histology,...
Adrenocortical cancer is one of the most aggressive endocrine malignancies. Growth through capsule or accidental release cells during surgery frequently results in metastatic disease. We investigated antitumoral effect 2 adrenocorticolytic compounds, O, P'-DDD and MeSO2-DDE, adrenocortical cell line H295R both vitro as a xenograft model vivo. were injected s. c. nude mice. P'-DDD, oil (control) was administered i. p., either simultaneously with injection at day 0 (mimicking adjuvant...
Abstract Essential hypertension occurs in approximately 25% of the adult population and one cause is primary aldosteronism. Targeting angiotensin II AT 1 receptor using PET an appropriate tracer may offer a diagnostic method for adrenocortical tissue. This report describes synthesis selective antagonist [ carboxyl ‐ 11 C]eprosartan 10, 4‐[2‐butyl‐5‐(( E )‐2‐carboxy‐3‐thiophen‐2‐yl‐propenyl)‐imidazol‐1‐ylmethyl]‐[ C]benzoic acid, its precursor ( )‐3‐[2‐butyl‐3‐(4‐iodo‐benzyl)‐3 H...
Abstract Background Considering the width and importance of using Multicellular Tumor Spheroids (MTS) in oncology research, size determination MTSs by an accurate fast method is essential. In present study effective, semi-automated method, SASDM, was developed to determinate MTSs. The applied tested three different cell-lines. Frozen section autoradiography Hemotoxylin Eosin (H&E) staining used for further confirmation. Results SASDM shown be user-friendly, time efficient, more precise...
Zolmitriptan is a serotonin<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mn>5</mml:mn><mml:mtext>-</mml:mtext><mml:msub><mml:mrow><mml:mtext>HT</mml:mtext></mml:mrow><mml:mrow><mml:mn>1</mml:mn><mml:mtext>B</mml:mtext><mml:mo>/</mml:mo><mml:mn>1</mml:mn><mml:mtext>D</mml:mtext></mml:mrow></mml:msub></mml:math>receptor agonist that an effective and well-tolerated drug for migraine treatment. In human positron emission tomography study, [ 11 C]zolmitriptan crossed the...
Abstract Background The previously validated NK 1 -receptor ligand [ O - methyl 11 C]GR205171 binds with a high affinity to the and displays slow dissociation from receptor. Hence, it cannot be used in vivo for detecting concentration changes substance P, endogenous -receptor. A radioligand monitoring these has enable displacement by thus bind reversibly Small structure of receptor can lead binding characteristics also ability penetrate blood-brain barrier. aim this study was use carbon-11...
The persistent adrenocorticolytic DDT metabolite 3-methylsulfonyl-DDE (MeSO(2)-DDE) was originally identified in Baltic grey seals, a population suffering from adrenocortical hyperplasia. In mice, MeSO(2)-DDE induces mitochondrial degeneration and cellular necrosis the adrenal zona fasciculata. this study, we used precision-cut tissue slice culture to examine local CYP11B1-catalyzed irreversible binding of murine cortex. We also examined effects on steroid hormone secretion, histology,...
Abstract Clinical findings using [ 11 C]methyl 1‐[(1 R )‐1‐phenylethyl]‐1 H ‐imidazole‐5‐carboxylate ([ C]MTO, 1) show high uptake in lesions of adrenocortical origin, including adenomas, but low non‐adrenocortical origin. In this paper the synthesis and preclinical evaluation two new C‐labelled analogues MTO, )‐1‐(4‐chlorophenyl)ethyl]‐1 C]CLM, 2) )‐1‐(4‐bromophenyl)ethyl]‐1 C]BRM, 3), frozen‐section autoradiography, organ distribution a metabolic study are presented. Copyright © 2008 John...
The persistent adrenocorticolytic DDT metabolite 3-methylsulfonyl-DDE (MeSO(2)-DDE) was originally identified in Baltic grey seals, a population suffering from adrenocortical hyperplasia. In mice, MeSO(2)-DDE induces mitochondrial degeneration and cellular necrosis the adrenal zona fasciculata. this study, we used precision-cut tissue slice culture to examine local CYP11B1-catalyzed irreversible binding of murine cortex. We also examined effects on steroid hormone secretion, histology,...
Abstract [18F]AH113804 is a peptide based molecular imaging agent with high affinity for the human c-Met receptor. The objective of this study was to investigate biodistribution, specific uptake and elimination in non-human primate. Cynomolgus monkey (Macaca fascicularis) selected since retains species. route administration intravenous as humans. target compound prepared two-step synthesis starting 4-[18F]fluorobenzaldehyde which reacted precursor produce corresponding oxime. Following...