A5070191512- Parasitic Infections and Diagnostics
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Amoebic Infections and Treatments
- Research in Social Sciences
- Microbial Community Ecology and Physiology
- T-cell and B-cell Immunology
- Sustainable Development and Environmental Policy
- Political Economy and Marxism
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Immunotherapy and Immune Responses
- Insect symbiosis and bacterial influences
- Photosynthetic Processes and Mechanisms
- Income, Poverty, and Inequality
- Mollusks and Parasites Studies
- Aquaculture disease management and microbiota
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- European Union Policy and Governance
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- Agricultural Economics and Policy
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
Uppsala University
2012-2025
Åbo Akademi University
1986-2021
Science for Life Laboratory
2011-2016
University of Gothenburg
2011
Dalhousie University
2000-2007
Canadian Institute for Advanced Research
2001-2004
Linköping University Hospital
2003
Giardia intestinalis is a major cause of diarrheal disease worldwide and two genotypes, assemblages A B, infect humans. The genome assemblage parasite WB was recently sequenced, the structurally compact 11.7 Mbp contains simplified basic cellular machineries metabolism. We here performed 454 sequencing to 16x coverage B isolate GS, only successfully used experimentally animals genomes show 77% nucleotide 78% amino-acid identity in protein coding regions. Comparative analysis identified 28...
During B-cell development the surrogate light (SL) chain is selectively expressed in progenitor and precursor B cells during developmental stages of D(H) to J(H) V(H) D(H)J(H) rearrangements. Approximately half all muH chains produced by these rearrangements cannot pair with SL form a pre-B-cell receptor (pre-BCR). A spectrum affinities between VpreB individual domains generates preB pre-BCR different fitness which, turn, determines extent pre-B II-cell proliferation fidelity allelic...
Frequencies of mitogen-reactive B cells have been determined in vitro under culture conditions which allow every growth-inducible cell to grow and mature into a clone Ig-secreting PFC. The frequencies LPS-reactive the spleen 6- 8-wk old mice were between 1 3 10 splenic from following inbred strains mice: C3H/Tif; BALB/c; BALB/c nu/nu; C57BL/6J; DBA/2J; C57BL/6J x DBA/(2J)F(1); CBA A/J. Very similar are found for lipoprotein-reactive BALB/c, nu/nu, C3H/Tif, C3H/HeJ mice. No but normal mice,...
Abstract Background Giardia intestinalis is a protozoan parasite that causes diarrhea in wide range of mammalian species. To further understand the genetic diversity between species, we have performed genome sequencing and analysis wild-type sample from assemblage E group, isolated pig. Results We identified 5012 protein coding genes, majority which are conserved compared to previously sequenced genomes WB GS strains terms microsynteny sequence identity. Despite this, there an unexpectedly...
Background Giardia intestinalis is one of the most common diarrhea-related parasites in humans, where infection ranges from asymptomatic to acute or chronic disease. G. consists eight genetically distinct genotypes assemblages, designated A–H, and assemblages A B can infect humans. Giardiasis has been classified as a possible zoonotic disease but role animals human transmission still needs be proven. We tried link different sub-assemblages isolates Swedish patients clinical symptoms...
To study reductive evolutionary processes in bacterial genomes, we examine sequences the Rickettsia genomes which are unconstrained by selection and evolve as pseudogenes, one of is metK gene, codes for AdoMet synthetase. Here, sequenced gene three surrounding genes eight different species genus Rickettsia. The was found to contain a high incidence deletions six lineages, while its surroundings were functionally conserved all lineages. A more drastic example degradation identified downstream...
Studies of neutrally evolving sequences suggest that differences in eukaryotic genome sizes result from different rates DNA loss. However, very few pseudogenes have been identified microbial species, and the processes whereby genes genomes deteriorate bacteria remain largely unresolved. The typhus-causing agent, Rickettsia prowazekii, is exceptional as much 24% its 1.1-Mb consists noncoding pseudogenes. To test hypothesis R. prowazekii represents degraded remnants ancestral genes, we...
Background The protozoan parasite Giardia intestinalis and the pathogenic bacterium Helicobacter pylori are well known for their high prevalences in human hosts worldwide. prevalence of both organisms is to peak densely populated, low resource settings children infected early life. Different genotypes/assemblages have been associated with different symptoms H. induction cancer. Despite this, not much data available from sub-Saharan Africa regards G. assemblages potential association...
Spironucleus salmonicida causes systemic infections in salmonid fish. It belongs to the group diplomonads, binucleated heterotrophic flagellates adapted micro-aerobic environments. Recently we identified energy-producing hydrogenosomes S. salmonicida. Here present a genome analysis of fish parasite with focus on comparison more studied diplomonad Giardia intestinalis. We annotated 8067 protein coding genes ∼12.9 Mbp genome. Unlike G. intestinalis, promoter-like motifs were found upstream...
Abstract Background Lateral gene transfer (LGT) in eukaryotes from non-organellar sources is a controversial subject need of further study. Here we present distribution and phylogenetic analyses the genes encoding hybrid-cluster protein, A-type flavoprotein, glucosamine-6-phosphate isomerase, alcohol dehydrogenase E. These four have limited among sequenced prokaryotic eukaryotic genomes were previously implicated events affecting eukaryotes. If our previous contention that these introduced...
Comparative genomic studies of the mitochondrion-lacking protist group Diplomonadida (diplomonads) has been lacking, although Giardia lamblia intensively studied. We have performed a sequence survey project resulting in 2341 expressed tags (EST) corresponding to 853 unique clones, 5275 genome sequences (GSS), and eleven finished contigs from diplomonad fish parasite Spironucleus salmonicida (previously described as S. barkhanus). The analyses revealed compact with few, if any, introns very...
Abstract Diplomonads are anaerobic, flagellated protists, being part of the Metamonada group Eukaryotes. either live as endobionts (parasites and commensals) animals or free-living in low-oxygen environments. Genomic information is available for parasitic diplomonads like Giardia intestinalis Spironucleus salmonicida , while little known about genomic arrangements diplomonads. We have generated first reference genome a diplomonad, Hexamita inflata . The final version assembly fragmented...
The rainbow trout (Oncorhynchus mykiss) is one of the most widely used fish species in aquaculture and physiological research. In present paper, we report first cloning, 3D (three-dimensional) modelling, pharmacological characterization tissue distribution two melanocortin (MC) receptors trout. Phylogenetic analysis indicates that these are orthologues human MC4 MC5 receptors. We created molecular models their counterparts. These suggest greater divergence between than analyses demonstrated...