A5038081903- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Parasitic Infections and Diagnostics
- Methane Hydrates and Related Phenomena
- Parasite Biology and Host Interactions
- Marine Biology and Ecology Research
- Amoebic Infections and Treatments
- Marine Sponges and Natural Products
- Reproductive tract infections research
- Legume Nitrogen Fixing Symbiosis
- Marine Ecology and Invasive Species
- Marine and coastal plant biology
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Legionella and Acanthamoeba research
- Paleontology and Stratigraphy of Fossils
- Environmental DNA in Biodiversity Studies
- Vector-borne infectious diseases
- Myxozoan Parasites in Aquatic Species
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- Infectious Diseases and Mycology
- Coastal wetland ecosystem dynamics
- Botanical Research and Applications
Charles University
2016-2025
Výzkumná Stanice Vinohradnická
2024
University of Cambridge
2023
Abstract This revision of the classification eukaryotes follows that Adl et al., 2012 [ J. Euk. Microbiol . 59(5)] and retains an emphasis on protists. Changes since have improved resolution many nodes in phylogenetic analyses. For some clades even families are being clearly resolved. As we had predicted, environmental sampling intervening years has massively increased genetic information at hand. Consequently, discovered novel clades, exciting new genera uncovered a massive species level...
A group of protist experts proposes a two-step DNA barcoding approach, comprising universal eukaryotic pre-barcode followed by group-specific barcodes, to unveil the hidden biodiversity microbial eukaryotes.
Amoebozoa is the eukaryotic supergroup sister to Obazoa, lineage that contains animals and Fungi, as well their protistan relatives, breviate apusomonad flagellates. extraordinarily diverse, encompassing important model organisms significant pathogens. Although amoebozoans are integral global nutrient cycles present in nearly all environments, they remain vastly understudied. We a robust phylogeny of based on broad representative set taxa phylogenomic framework (325 genes). By sampling 61...
Discoveries of diverse microbial eukaryotes and their inclusion in comprehensive phylogenomic analyses have crucially re-shaped the eukaryotic tree life 21st century.1Burki F. Roger A.J. Brown M.W. Simpson A.G.B. The New Tree Eukaryotes.Trends Ecol. Evol. 2020; 35: 43-55Abstract Full Text PDF PubMed Scopus (216) Google Scholar At deepest level, diversity comprises 9–10 "supergroups." One these supergroups, Metamonada, is particularly important to our understanding evolutionary dynamics...
Apicomplexa is a diverse phylum comprising unicellular endobiotic animal parasites and contains some of the most well-studied microbial eukaryotes including devastating human pathogens Plasmodium falciparum Cryptosporidium hominis. In contrast, data on invertebrate-infecting gregarines remains sparse their evolutionary relationship to other apicomplexans obscure. Most retain highly modified plastid, while mitochondria remain metabolically conserved. spp. inhabit an anaerobic host-gut...
Three strains of a trypanosomatid protozoan were isolated from the midguts two naturally infected species biting midges [ Culicoides ( Oecacta ) festivipennis and truncorum ] characterized by light electron microscopy molecular techniques. Morphological characteristics sequences 18S rRNA, 5S spliced leader RNA glycosomal glyceraldehyde-3-phosphate dehydrogenase genes indicate that studied flagellates represent novel phylogenetic lineage within Trypanosomatidae. Based on analyses,...
Trypanosomes belonging to Trypanosoma theileri group are mammalian blood parasites with keds and horse fly vectors. Our aim is study vector specificity of T. trypanosomes. During our bloodsucking Diptera survey, we found a surprisingly high prevalence trypanosomes in mosquitoes (154/4051). Using PCR gut dissections, detected mainly Aedes mosquitoes, the highest Ae. excrucians (22%), punctor (21%), cantans/annulipes (10%). Moreover, were blackflies, which reported as potential vectors for...
Pelomyxa is a genus of anaerobic amoebae that live in consortia with multiple prokaryotic endosymbionts. Although the symbionts represent large fraction cellular biomass, their metabolic roles have not been investigated. Using single-cell genomics and transcriptomics, we characterized community associated P. schiedti, which composed two bacteria, Candidatus Syntrophus pelomyxae (class Deltaproteobacteria) Vesiculincola Clostridia), methanogen, Methanoregula pelomyxae. Fluorescence situ...
Multiple prokaryotic lineages use the arginine deiminase (ADI) pathway for anaerobic energy production by degradation. The distribution of this among eukaryotes has been thought to be very limited, with only two specialized groups living in low oxygen environments (Parabasalia and Diplomonadida) known possess complete set all three enzymes. We have performed an extensive survey available sequence data order map these enzymes reconstruct their phylogenies. found genes almost examined...
Mitochondria and peroxisomes are the two organelles that most affected during adaptation to microoxic or anoxic environments. known transform into anaerobic mitochondria, hydrogenosomes, mitosomes, various transition stages in between, collectively called mitochondrion-related (MROs), which vary enzymatic capacity. Anaerobic were identified only recently, their putatively conserved function seems be metabolism of inositol. The group Archamoebae includes anaerobes bearing both MROs,...
Abstract The morphology, morphogenesis, and molecular phylogeny of a new metopid ciliate, Castula specialis sp. nov., comprising three strains from geographically distant (China, Mexico, Czech Republic) anoxic freshwater habitats, were studied based on microscopic observation live protargol‐stained specimens as well SSU rRNA gene sequence data. species is characterized follows: size in vivo 105–220 × 25–70 μm, body oblong to elongated ellipsoidal asymmetrical; preoral dome distinctly...
We present the first molecular phylogenetic examination of evolutionary position retortamonads, a group mitochondrion-lacking flagellates usually found as commensals intestinal tracts vertebrates. Our phylogenies include small subunit ribosomal gene sequences from six retortamonad isolates—four mammals and two amphibians. All were highly similar (95%–99%), with those being almost identical to each other. methods utilized unequivocally placed retortamonads another amitochondriate group,...
To clarify the taxonomy of trichomonads associated with human respiratory diseases, we examined a collection axenic trichomonad strains isolated from oral cavity and bronchi patients pulmonary diseases clinics in Tallin, Estonia. The bronchial were compared mutually as well reference strain Trichomonas tenax, common inhabitant cavity, other species humans animals. Unexpectedly, morphological studies, DNA sequencing ITS1-5.8S rRNA-ITS2 regions revealed that Estonian belong to genus...
Abstract Background Fornicata is a relatively recently established group of protists that includes the diplokaryotic diplomonads (which have two similar nuclei per cell), and monokaryotic enteromonads, retortamonads Carpediemonas , with more typical one nucleus cell. The monophyly was confirmed by molecular phylogenetic studies, but neither internal phylogeny nor its position on eukaryotic tree has been clearly resolved. Results Here we introduced data for three genes (SSU rRNA, α-tubulin...