A5034345913- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Legionella and Acanthamoeba research
- Bacteriophages and microbial interactions
- Marine Ecology and Invasive Species
- Social and Educational Sciences
- Lexicography and Language Studies
- Invertebrate Immune Response Mechanisms
- Animal Genetics and Reproduction
- Parasitic infections in humans and animals
- RNA Interference and Gene Delivery
- Myxozoan Parasites in Aquatic Species
- Advanced biosensing and bioanalysis techniques
- Insect Utilization and Effects
- MXene and MAX Phase Materials
- Enterobacteriaceae and Cronobacter Research
- Viral gastroenteritis research and epidemiology
- Child Development and Digital Technology
- Mitochondrial Function and Pathology
- Toxoplasma gondii Research Studies
University of Cambridge
2019-2022
Uppsala University
2012-2021
Science for Life Laboratory
2014
Abstract Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement eight other as...
Differentiation into infectious cysts through the process of encystation is crucial for transmission and survival intestinal protozoan parasite Giardia intestinalis. Hitherto majority studies have focused on early events, leaving late poorly defined. In order to further study encystation, focusing later we developed a new protocol that generates higher yield mature compared standard methods. Transcriptome changes during entire differentiation from trophozoites were thereafter studied using...
Spironucleus salmonicida causes systemic infections in salmonid fish. It belongs to the group diplomonads, binucleated heterotrophic flagellates adapted micro-aerobic environments. Recently we identified energy-producing hydrogenosomes S. salmonicida. Here present a genome analysis of fish parasite with focus on comparison more studied diplomonad Giardia intestinalis. We annotated 8067 protein coding genes ∼12.9 Mbp genome. Unlike G. intestinalis, promoter-like motifs were found upstream...
Giardia intestinalis is a common cause of diarrheal disease and it consists eight genetically distinct genotypes or assemblages (A-H). Only A B infect humans are suggested to represent two different species. Correlations exist between assemblage type host-specificity some extent symptoms. Phenotypical differences have been documented genome sequences available for A, E. We characterized compared the polyadenylated transcriptomes Four isolates were studied (WB (AI), AS175 (AII), P15 (E) GS...
Acquisition of the mitochondrion is a key event in evolution eukaryotic cell, but diversification organelle has occurred during evolution. One example such mitochondria-related organelles (MROs) are hydrogenosomes, which produce ATP by substrate-level phosphorylation with hydrogen as byproduct. The diplomonad parasite Giardia intestinalis harbours mitosomes, another type MRO. Here we identify MROs salmon Spironucleus salmonicida similar protein import and Fe–S cluster assembly machineries...
Diplomonad parasites of the genus Giardia have adapted to colonizing different hosts, most notably intestinal tract mammals. The human-pathogenic species, intestinalis, has been extensively studied at genome and gene expression level, but no such information is available for other species. Comparative data would be particularly valuable muris, which colonizes mice commonly used as a prototypic in vivo model investigating host responses parasitic infection. Here we report draft-genome G....
Mitochondria of opisthokonts undergo permanent fission and fusion throughout the cell cycle. Here, we investigated dynamics mitosomes, simplest forms mitochondria, in anaerobic protist parasite Giardia intestinalis, a member Excavata supergroup eukaryotes. The mitosomes have abandoned typical mitochondrial traits such as genome aerobic respiration their single role known to date is formation iron–sulfur clusters. In live experiments, no events were observed between G. intestinalis. Moreover,...
Eukaryotic microbes are highly diverse, and many lineages remain poorly studied. One such lineage, the diplomonads, a group of binucleate heterotrophic flagellates, has been studied mainly due to impact Giardia intestinalis, an intestinal, diarrhea-causing parasite in humans animals. Here we describe development stable transfection system for use Spironucleus salmonicida, diplomonad that causes systemic spironucleosis salmonid fish. We designed vectors cassette format carrying epitope tags...
Giardia intestinalis is an intestinal protozoan parasite that causes diarrheal infections worldwide. A key process to sustain its chain of transmission the formation infectious cysts in encystation process. We combined deep RNAseq a broad range timepoints produce high-resolution gene expression map encystation. This detailed transcriptomic confirmed gradual change along time course encystation, showing most significant changes during late Few genes are differentially expressed early but...
Annexins are multifunctional, calcium-binding proteins found in organisms across all kingdoms. Most studies of annexins from single-celled eukaryotes have focused on the alpha-giardins, assigned to group E annexins, expressed by diplomonad Giardia intestinalis. We characterized annexin gene family another parasite, Spironucleus salmonicida, phylogenetic and experimental approaches. constructed a comprehensive phylogeny that they abundant with frequent duplications losses. The S. salmonicida...
Abstract Mitochondrial genomes of apicomplexans, dinoflagellates, and chrompodellids that collectively make up the Myzozoa, encode only three proteins (Cytochrome b [COB], Cytochrome c oxidase subunit 1 [COX1], 3 [COX3]), contain fragmented ribosomal RNAs, display extensive recombination, RNA trans-splicing, RNA-editing. The early-diverging Perkinsozoa is final major myzozoan lineage whose mitochondrial remained poorly characterized. Previous reports Perkinsus genes indicated independent...
The phylum Perkinsozoa is an aquatic parasite lineage that has devastating effects on commercial and natural mollusc populations, also comprises parasites of algae, fish amphibians. They are related to dinoflagellates apicomplexans thus offer excellent genetic models for both parasitological evolutionary studies. Genetic transformation was previously achieved Perkinsus spp. but with few tools transgene expression limited selection efficacy. We sought expand the power experimental using P....
ABSTRACT Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement 8 other as well...
Abstract Diplomonad parasites of the genus Giardia have adapted to colonizing different hosts, most notably intestinal tract mammals. The human-pathogenic species, intestinalis , has been extensively studied at genome and gene expression level, but no such information is available for other species. Comparative data would be particularly valuable muris which colonizes mice commonly used as a prototypic in vivo model investigating host responses parasitic infection. Here we report...
We have created plasmids for transfection of the parasite Perkinsus marinus using Golden Gate cloning. explored possibility to either FACS sort (GFP/mCherry fused MOE membrane protein) or drug selection (bleomycin, blasticidin S puromycin) enrich transfected cells.
Perkinsus species are important marine parasites of molluscs including mussels and oysters with substantial commercial environmental impact. forms a sister lineage to the apicomplexan (e.g. malaria agent, Plasmodium) share similar invasion-related structures these better studied pathogens. However, much less is known transmission invasion biology spp. We developing genetic tools study parasites, emerging as an experimental model for parasites. The life cycle start motile zoospores that...