A5057204719- Microbial Community Ecology and Physiology
- Viral gastroenteritis research and epidemiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- Environmental DNA in Biodiversity Studies
- Marine Biology and Ecology Research
- Atmospheric chemistry and aerosols
- Viral Infectious Diseases and Gene Expression in Insects
- Marine Ecology and Invasive Species
- Plant responses to elevated CO2
- Ocean Acidification Effects and Responses
- Allelopathy and phytotoxic interactions
- Animal Virus Infections Studies
- Plant Growth Enhancement Techniques
- Air Quality and Health Impacts
- Diatoms and Algae Research
- Plant Virus Research Studies
- Atmospheric Ozone and Climate
- Neurobiology and Insect Physiology Research
- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Plant Parasitism and Resistance
- Vector-Borne Animal Diseases
University of Minnesota
2021-2024
University of Minnesota System
2023
Marine Biological Association of the United Kingdom
2014-2020
University of Southampton
2019-2020
University of Oxford
2014
Abstract Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement eight other as...
The particle structure of Emiliania huxleyi virus (EhV), an algal infecting member nucleocytoplasmic large DNA viruses (NCLDVs), contains outer lipid membrane envelope similar to that found in animal such as African swine fever (ASFV). Despite both being enveloped NCLDVs, EhV and ASFV are known for their stability outside host environment.
Introduction African swine fever virus (ASFV) is extremely stable in the environment, and previous laboratory experiments simulations have also shown it to be highly animal feed ingredients. However, ASFV cannot studied real world demonstrations because a contagious virus. member of nucleocytoplasmic large DNA viruses (NCLDVs), similar Emiliania huxleyi (EhV), which has restricted host range limited species marine algae called . This algal NCLDV many morphological physical characteristics...
Abstract. Due to the unprecedented rate at which our climate is changing, ultimate consequence for many species likely be either extinction or migration an alternate habitat. Certain might, however, evolve a that could make them resilient effects of rapidly changing environment. This scenario most apply have large population sizes and rapid generation times, such genetic variation required adaptive evolution can readily supplied. Emiliania huxleyi (Lohm.) Hay Mohler (Prymnesiophyceae)...
Microbes occupy diverse ecological niches and only through recent advances in next generation sequencing (NGS) technologies has the true microbial diversity been revealed. Furthermore, lack of perceivable marine barriers to genetic dispersal (i.e. mountains or islands) allowed speculation that organisms can be easily transported by currents therefore proliferate everywhere. That said, ocean fronts are now commonly being recognised as for dispersal. Here we analysed samples collected from a...
African swine fever virus (ASFV) is a member of the nucleocytoplasmic large DNA viruses (NCLDVs) and stable in variety environments, including animal feed ingredients as shown previous laboratory experiments simulations.
The aquatic microbiome is composed of a multi-phylotype community microbes, ranging from the numerically dominant viruses to phylogenetically diverse unicellular phytoplankton. They influence key biogeochemical processes and form base marine food webs, becoming for secondary consumers. Due recent advances in next-generation sequencing, this previously overlooked component our hydrosphere starting reveal its true diversity biological complexity. We report here that 250 mL seawater sufficient...
Abstract Increasing influence of Atlantic water in the Arctic Ocean has potential to significantly impact regional temperature and salinity. Here we use a rDNA barcoding approach reveal how microbial communities are partitioned into distinct assemblages across gradient Atlantic-Polar Water Norwegian Sea. Data suggest that temperate adapted bacteria may replace cold taxa under future scenario increasing influence, but eukaryote response is more complex. Some abundant eukaryotic could persist,...
Abstract The fate of coccolithophores in the future oceans remains uncertain, part due to key factors having not been standardized across experiments. A potentially moderating role for differences day length (photoperiod) largely unexplored. We therefore cultured four different geographical isolates species Emiliania huxleyi , as well two additional species, Gephyrocapsa oceanica (tropical) and Coccolithus braarudii (temperate), test interactive effects p CO 2 with light : dark (L D) cycle....
The rapid advancement of next generation sequencing protocols in recent years has led to the diversification methods used study microbial communities; however, how comparable data generated from these different are, remains unclear. In this we compared taxonomic composition and seasonal dynamics bacterial community determined by two distinct 16s amplicon protocols: V6 region rRNA gene using 454 pyrosequencing vs V4 Illumina Hiseq 2500 platform. Significant differences between relative...
Porcine reproductive and respiratory syndrome virus (PRRSV) continues to mutate, causing disruptive PRRS outbreaks in farms that lead failure disease-associated mortality. We present four new PRRSV type 2 variants the United States belonging distinct orf5 sublineages within lineage 1.
Abstract. Due to the unprecedented rate at which our climate is changing, ultimate consequence for many species likely be either extinction or migration an alternate habitat. Certain might, however, evolve a that could make them resilient effects of rapidly changing environment. This scenario most apply have large population sizes and rapid generation times, such genetic variation required adaptive evolution can readily supplied. Emiliania huxleyi (Lohm.) Hay Mohler (Prymnesiophyceae) as it...
Abstract Background: The particle structure of Emiliania huxleyi virus (EhV), an algal infecting member nucleocytoplasmic large DNA viruses (NCLDVs), contains outer lipid membrane envelope similar to that found in animal such as African swine fever (ASFV). Despite both being enveloped NCLDVs, EhV and ASFV are known for their stability the environment. Method: Here we report first time, application a viability PCR method describe unprecedented virion thermal ASFV. This result contradicts...
ABSTRACT Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement 8 other as well...