A5077599740- Protist diversity and phylogeny
- Parasitic Infections and Diagnostics
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Amoebic Infections and Treatments
- Photosynthetic Processes and Mechanisms
- Biochemical and Molecular Research
- Metalloenzymes and iron-sulfur proteins
- Marine Ecology and Invasive Species
- Trypanosoma species research and implications
- Mitochondrial Function and Pathology
- Bacterial Genetics and Biotechnology
- Evolution and Genetic Dynamics
- Trace Elements in Health
- Biotin and Related Studies
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Microbial Fuel Cells and Bioremediation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
Charles University
2011-2023
Czech Academy of Sciences, Biology Centre
2020-2023
Institute of Parasitology
2020-2023
University of Ostrava
2023
University of Cambridge
2023
University of Kent
2019-2021
Výzkumná Stanice Vinohradnická
2009
Abstract Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement eight other as...
The mitosomes of Giardia intestinalis are thought to be mitochondria highly-reduced in response the oxygen-poor niche. We performed a quantitative proteomic assessment increase understanding function and evolutionary origin these enigmatic organelles. Mitosome-enriched fractions were obtained from cell homogenate using Optiprep gradient centrifugation. To distinguish mitosomal proteins contamination, we used shot-gun strategy based on isobaric tagging peptides with iTRAQ tandem mass...
We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 these, localization had not been described before. use TrypTag dataset as foundation and take advantage properties fluorescent protein tag that causes aberrant but fortuitous accumulation tagged matrix inner membrane proteins near kinetoplast (mitochondrial DNA). Combined with transmembrane domain predictions, this characteristic allowed...
Giardia intestinalis parasites contain mitosomes, one of the simplest mitochondrion-related organelles. Strategies to identify functions mitosomes have been limited mainly homology detection, which is not suitable for identifying species-specific proteins and their functions. An in vivo enzymatic tagging technique based on Escherichia coli biotin ligase (BirA) has introduced G. intestinalis; this method allows compartment-specific biotinylation a protein interest. Known involved mitosomal...
Abstract The type 2 secretion system (T2SS) is present in some Gram-negative eubacteria and used to secrete proteins across the outer membrane. Here we report that certain representative heteroloboseans, jakobids, malawimonads hemimastigotes unexpectedly possess homologues of core T2SS components. We show at least them are mitochondria, their behaviour biochemical assays consistent with presence a mitochondrial T2SS-derived (miT2SS). additionally identified 23 protein families co-occurring...
The notion that mitochondria cannot be lost was shattered with the report of an oxymonad Monocercomonoides exilis, first eukaryote arguably without any mitochondrion. Yet, questions remain about whether this extends beyond single species and how transition took place. Oxymonadida is a group gut endobionts taxonomically housed in Preaxostyla which also contains free-living flagellates genera Trimastix Paratrimastix. latter two taxa harbour conspicuous mitochondrion-related organelles (MROs)....
The transition of free-living organisms to parasitic is a mysterious process that occurs in all major eukaryotic lineages. Parasites display seemingly unique features associated with their pathogenicity; however, it important distinguish ancestral preconditions parasitism from truly new parasite-specific functions. Here, we sequenced the genome and transcriptome anaerobic Mastigamoeba balamuthi performed phylogenomic analysis four related members Archamoebae, including Entamoeba histolytica,...
Iron-sulfur (Fe-S) clusters are essential cofactors that enable proteins to transport electrons, sense signals, or catalyze chemical reactions. The maturation of dozens Fe-S in various compartments every eukaryotic cell is driven by several assembly pathways. ubiquitous cytosolic cluster (CIA) pathway, typically composed eight highly conserved proteins, depends on mitochondrial (ISC) machinery. Giardia intestinalis contains one the smallest genomes and mitosome, an extremely reduced...
The anaerobic intestinal pathogen Giardia intestinalis does not possess enzymes for heme synthesis, and it also lacks the typical set of hemoproteins that are involved in mitochondrial respiration cellular oxygen stress management. Nevertheless, G. may require function particular hemoproteins, such as cytochrome b5 (cytb5). We have analyzed sequences eukaryotic cytb5 proteins identified three distinct groups: group I, which consists C-tail membrane-anchored proteins; II, includes soluble...
The main bacterial pathway for inserting proteins into the plasma membrane relies on signal recognition particle (SRP), composed of Ffh protein and an associated RNA component, SRP-docking FtsY. Eukaryotes use equivalent system archaeal origin to deliver endoplasmic reticulum, whereas a bacteria-derived SRP FtsY function in plastid. Here we report presence homologs various unrelated plastid-lacking unicellular eukaryotes, namely Heterolobosea, Alveida, Goniomonas, Hemimastigophora. monophyly...
The highly reduced mitochondria (mitosomes) of Giardia intestinalis are recently discovered organelles for which, it was suggested, iron-sulfur cluster assembly their only conserved function. However, an incomplete set the components required FeS biogenesis localized to mitosomes. Via proteomic analysis a mitosome-rich cellular fraction together with immunofluorescence microscopy, we identified novel mitosomal protein homologous monothiol glutaredoxins containing CGFS motif at active site....
Abstract Background Nbp35-like proteins (Nbp35, Cfd1, HCF101, Ind1, and AbpC) are P-loop NTPases that serve as components of iron-sulfur cluster (FeS) assembly machineries. In eukaryotes, Ind1 is present in mitochondria, its function associated with the FeS clusters subunits respiratory Complex I, Nbp35 Cfd1 cytosolic (CIA) pathway, HCF101 involved photosystem I plastids plants (chHCF101). The AbpC protein operates Bacteria Archaea. To date, cellular distribution these considered to be...
ZapE/Afg1 is a component of the inner cell membrane some eubacteria and mitochondrial eukaryotes. This protein involved in FtsZ-dependent division eubacteria. In yeast human mitochondrion, likely interacts with Oxa1 facilitates degradation mitochondrion-encoded subunits respiratory complexes. Furthermore, depletion ZapE increases resistance to apoptosis, decreases oxidative stress tolerance, impacts homeostasis. It remains unclear whether multifunctional protein, or described effects are...
ABSTRACT Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement 8 other as well...
Abstract The notion that mitochondria cannot be lost was shattered with the report of an oxymonad Monocercomonoides exilis , first eukaryote arguably without any mitochondrion. Yet, questions remain about whether this extends beyond single species and how transition took place. Oxymonadida is a group gut endobionts taxonomically housed in Preaxostyla which also contains free-living flagellates genera Trimastix Paratrimastix . latter two taxa harbour conspicuous mitochondrion-related...
Abstract The type 2 secretion system (T2SS) is present in some Gram-negative eubacteria and used to secrete proteins across the outer membrane. Here we report that certain representative heteroloboseans, jakobids, malawimonads hemimastigotes unexpectedly possess homologues of core T2SS components. We show at least them are mitochondria, their behaviour biochemical assays consistent with presence a mitochondrial T2SS-derived (miT2SS). additionally identified 23 protein families co-occurring...
A range of recently developed techniques that take advantage proximity biotinylation, such as BioID, TurboID, and APEX, are particularly suitable for the studies protein-protein interactions. As compared to classical co-immunoprecipitation, their is higher reproducibility capacity identify stable complexes also capture transient Briefly, protein interest fused with modified biotin ligase, which promiscuously biotinylates proteins in its proximity. However, until now BioID has been used T....
Abstract Background: Nbp35-like proteins (Nbp35, Cfd1, HCF101, Ind1, and AbpC) are P-loop NTPases that serve as components of iron-sulfur cluster (FeS) assembly machineries. In eukaryotes, Ind1 is present in mitochondria, its function associated with the FeS clusters subunits respiratory Complex I, Nbp35 Cfd1 cytosolic (CIA) pathway, HCF101 involved photosystem I plastids plants (chHCF101). The AbpC protein operates Bacteria Archaea. To date, cellular distribution these considered to be...