Ondřej Šmíd
A5072806184- Cardiac Arrest and Resuscitation
- Mechanical Circulatory Support Devices
- Respiratory Support and Mechanisms
- Parasitic Infections and Diagnostics
- Intensive Care Unit Cognitive Disorders
- Amoebic Infections and Treatments
- Thermal Regulation in Medicine
- Sepsis Diagnosis and Treatment
- Cardiac Structural Anomalies and Repair
- Metalloenzymes and iron-sulfur proteins
- Trauma and Emergency Care Studies
- Trauma Management and Diagnosis
- Venous Thromboembolism Diagnosis and Management
- Atrial Fibrillation Management and Outcomes
- Acute Myocardial Infarction Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiac electrophysiology and arrhythmias
- Trypanosoma species research and implications
- Cardiac Ischemia and Reperfusion
- Traumatic Brain Injury Research
- Cerebrovascular and Carotid Artery Diseases
- RNA modifications and cancer
- Viral Infections and Immunology Research
- Acute Ischemic Stroke Management
- Eosinophilic Disorders and Syndromes
General University Hospital in Prague
2014-2025
Charles University
2016-2025
Skåne University Hospital
2021
Lund University
2021
Jan Evangelista Purkyně University in Ústí nad Labem
2020
Beijing Anzhen Hospital
2017
Capital Medical University
2017
Guy's and St Thomas' NHS Foundation Trust
2017
Institute of Molecular Genetics
2016
Výzkumná Stanice Vinohradnická
2009
Unconscious survivors of out-of-hospital cardiac arrest have a high risk death or poor neurologic function. Therapeutic hypothermia is recommended by international guidelines, but the supporting evidence limited, and target temperature associated with best outcome unknown. Our objective was to compare two temperatures, both intended prevent fever.In an trial, we randomly assigned 950 unconscious adults after presumed cause targeted management at either 33°C 36°C. The primary all-cause...
Targeted temperature management is recommended for patients after cardiac arrest, but the supporting evidence of low certainty.In an open-label trial with blinded assessment outcomes, we randomly assigned 1900 adults coma who had out-of-hospital arrest presumed or unknown cause to undergo targeted hypothermia at 33°C, followed by controlled rewarming, normothermia early treatment fever (body temperature, ≥37.8°C). The primary outcome was death from any 6 months. Secondary outcomes included...
<h3>Importance</h3> Out-of-hospital cardiac arrest (OHCA) has poor outcome. Whether intra-arrest transport, extracorporeal cardiopulmonary resuscitation (ECPR), and immediate invasive assessment treatment (invasive strategy) is beneficial in this setting remains uncertain. <h3>Objective</h3> To determine whether an early approach adults with refractory OHCA improves neurologically favorable survival. <h3>Design, Setting, Participants</h3> Single-center, randomized clinical trial Prague,...
<h3>Objective:</h3> To identify reliable predictors of outcome in comatose patients after cardiac arrest using a single routine EEG and standardized interpretation according to the terminology proposed by American Clinical Neurophysiology Society. <h3>Methods:</h3> In this cohort study, 4 specialists, blinded outcome, evaluated prospectively recorded EEGs Target Temperature Management trial (TTM trial) that randomized 33°C vs 36°C. Routine was performed still rewarming. were classified into...
The mitosomes of Giardia intestinalis are thought to be mitochondria highly-reduced in response the oxygen-poor niche. We performed a quantitative proteomic assessment increase understanding function and evolutionary origin these enigmatic organelles. Mitosome-enriched fractions were obtained from cell homogenate using Optiprep gradient centrifugation. To distinguish mitosomal proteins contamination, we used shot-gun strategy based on isobaric tagging peptides with iTRAQ tandem mass...
Out of hospital cardiac arrest (OHCA) has a poor outcome. Recent non-randomized studies ECLS (extracorporeal life support) in OHCA suggested further prospective multicenter to define population that would benefit from ECLS. We aim perform randomized study comparing prehospital intraarrest hypothermia combined with mechanical chest compression device, intrahospital and early invasive investigation treatment all patients presumed origin compared standard care. This paper describes methodology...
Survival rates in refractory out-of-hospital cardiac arrest (OHCA) remain low with conventional advanced life support (ACLS). Extracorporeal (ECLS) implantation during ongoing resuscitation, a method called extracorporeal cardiopulmonary resuscitation (ECPR), may increase survival. This study examined whether ECPR is associated improved outcomes.Prague OHCA trial enrolled adults witnessed of presumed origin. In this secondary analysis, the effect on 180-day survival using Kaplan-Meier...
Abstract Background Basic management for patients who have suffered a cardiac arrest and are admitted to an intensive care unit (ICU) after resuscitation includes setting targets blood pressure managing sedation temperature. However, optimal unknown. Methods The STEPCARE (Sedation, Temperature Pressure Cardiac Arrest Resuscitation) trial is multicenter, parallel‐group, randomized, factorial, superiority in which sedation, temperature, strategies will be studied three separate comparisons...
Mitochondria are archetypal organelles of endosymbiotic origin in eukaryotic cells. Some unicellular eukaryotes (protists) were considered to be primarily amitochondrial organisms that diverged from the lineage before acquisition premitochondrial endosymbiont, but their status was recently challenged by discovery mitochondria-like double membrane-bound called mitosomes. Here, we report proteins targeted into mitosomes Giardia intestinalis have targeting signals necessary and sufficient...
BACKGROUND: The evidence for temperature control comatose survivors of cardiac arrest is inconclusive. Controversy exists as to whether the effects hypothermia differ per circumstances or patient characteristics. METHODS: An individual data meta-analysis Targeted Temperature Management at 33°C versus 36°C after Cardiac Arrest (TTM) and Hypothermia Normothermia Out-of-Hospital (TTM2) trials was conducted. intervention comparator normothermia. primary outcome all-cause mortality 6 months....
Abstract Background Randomized data evaluating the impact of extracorporeal cardiopulmonary resuscitation (ECPR) approach on long-term clinical outcomes in patients with refractory out-of-hospital cardiac arrest (OHCA) are lacking. The objective this follow-up study was to assess ECPR-based versus CCPR approach. Methods Prague OHCA trial a single-center, randomized, open-label trial. Patients witnessed presumed origin, without return spontaneous circulation, were randomized during ongoing...
Sevoflurane sample data in a standard single patient ICU room, VieCuri Medical Centre. Physical of the room: 52 m3, air refreshing rate minimum 6/hr
Background: No practical tool quantitates the risk of circulatory-etiology death (CED) immediately after successful cardiopulmonary resuscitation in patients without ST-segment–elevation myocardial infarction. We developed and validated a prediction model to rapidly determine that facilitate triage individualized treatment pathways. Methods: With use INTCAR (International Cardiac Arrest Registry), an 87-question data set representing 44 centers United States Europe, were classified as having...
The severity of tissue hypoxia is routinely assessed by serum lactate. We aimed to determine whether early lactate levels predict outcomes in refractory out-of-hospital cardiac arrest (OHCA) treated conventional and extracorporeal cardiopulmonary resuscitation (ECPR).This study a post-hoc analysis randomized Prague OHCA (NCT01511666) assessing ECPR (the group) or with prehospital achieved return spontaneous circulation ROSC group). Lactate concentrations measured on admission every 4 hours...
Transformation of the metabolically down-regulated mitochondrion mammalian bloodstream stage Trypanosoma brucei to ATP-producing insect procyclic is accompanied by de novo synthesis citric acid cycle enzymes and components respiratory chain. Because these metabolic pathways contain multiple iron-sulfur (FeS) proteins, their synthesis, including formation FeS clusters, required. However, nothing known about cluster biogenesis in trypanosomes, organisms that are evolutionarily distant from...
Mitochondrial processing peptidases are heterodimeric enzymes (alpha/betaMPP) that play an essential role in mitochondrial biogenesis by recognizing and cleaving the targeting presequences of nuclear-encoded proteins. The two subunits paralogues probably evolved duplication a gene for monomeric metallopeptidase from endosymbiotic ancestor mitochondria. Here, we characterize MPP-like proteins important human parasites contain highly reduced versions mitochondria, mitosomes Giardia...
The identification of mitosomes in Giardia generated significant debate on the evolutionary origin these organelles, whether they were highly reduced mitochondria or product a unique endosymbiotic event an amitochondrial organism. As protein import pathway is defining characteristic mitochondria, we sought to discover TOM (translocase outer mitochondrial membrane) complex Giardia. A Hidden Markov model search genome identified Tom40 homologous sequence (GiTom40), where translocation channel...
Fever is associated with brain injury after cardiac arrest. It unknown whether fever management a feedback-controlled device impacts patient-centered outcomes in arrest patients. This trial aims to investigate or without temperature control out-of-hospital The TEMP-CARE part of the 2 × factorial Sedation, TEmperature and Pressure Cardiac Arrest REsuscitation (STEPCARE) trial, randomized, international, multicenter, parallel-group, investigator-initiated, superiority that will evaluate...
Dysglycemia and glycemic variability are associated with poor outcomes in critically ill patients. Targeted temperature management alters blood glucose homeostasis. We investigated the association between concentrations neurologic of patients randomized to targeted at 33°C or 36°C after cardiac arrest.Post hoc analysis multicenter TTM-trial. Primary outcome this was 6 months, referred as "Cerebral Performance Category."Thirty-six sites Europe Australia.All 939 out-of-hospital arrest presumed...
Objectives: Despite a lack of randomized trials, practice guidelines recommend that mild induced hypothermia be considered for comatose survivors in-hospital cardiac arrest. This study describes the safety, feasibility, and outcomes treatment following Design: Prospective, observational, registry-based study. Setting: Forty-six critical care facilities in eight countries Europe United States reporting Hypothermia Network Registry International Cardiac Arrest Registry. Patients: A total 663...