A5020095277- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Marine Toxins and Detection Methods
- Marine Biology and Ecology Research
- Environmental DNA in Biodiversity Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Algal biology and biofuel production
- Marine Biology and Environmental Chemistry
- Diatoms and Algae Research
- Aquatic Invertebrate Ecology and Behavior
- Marine Ecology and Invasive Species
- Wastewater Treatment and Nitrogen Removal
- Isotope Analysis in Ecology
- Water Quality Monitoring and Analysis
- Lichen and fungal ecology
- Knee injuries and reconstruction techniques
- Polar Research and Ecology
- 14-3-3 protein interactions
- Connective tissue disorders research
- Silk-based biomaterials and applications
- Marine Bivalve and Aquaculture Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Botany and Plant Ecology Studies
- Ecology and Conservation Studies
University of Delaware
2016-2025
Houston Fertility Institute
2022
Wright State University
2015
Western Washington University
2011
College of Marin
1997-2008
South Carolina Department of Natural Resources
2005
North Carolina State University
2005
NOAA National Ocean Service
2005
University of South Carolina
2005
National Oceanic and Atmospheric Administration
2005
Current sampling of genomic sequence data from eukaryotes is relatively poor, biased, and inadequate to address important questions about their biology, evolution, ecology; this Community Page describes a resource 700 transcriptomes marine microbial help understand role in the world's oceans.
Harmful algal blooms (HABs) cause significant economic and ecological damage worldwide. Despite considerable efforts, a comprehensive understanding of the factors that promote these has been lacking, because biochemical pathways facilitate their dominance relative to other phytoplankton within specific environments have not identified. Here, biogeochemical measurements showed harmful alga Aureococcus anophagefferens outcompeted co-occurring in estuaries with elevated levels dissolved organic...
Abstract Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span diversity of eukaryotic tree life. However, genetic tractability has been limited to a few species, which do not represent or environmentally relevant taxa. Here, we report on development tools range protists primarily from marine environments. We present evidence for foreign DNA delivery and expression 13 species never before transformed advancement eight other as...
To adhere to solid surfaces, marine mussels produce byssal threads, each of which is a stiff tether at one end and shock absorber with 160 percent extensibility the other end. The elastic proximal byssus extraordinary given its construction collagen limited extension (less than 10 percent) most collagenous materials. From complementary DNA, we deduced that primary structure protein (preCol-P) predominating in extensible portion threads encodes an unprecedented natural block copolymer three...
Indirect, non-invasive detection of rare aquatic macrofauna using aqueous environmental DNA (eDNA) is a relatively new approach to population and biodiversity monitoring. As such, the sensitivity monitoring results different methods eDNA capture, extraction, being investigated in many ecosystems species. One first largest conservation programs with eDNA-based as central instrument focuses on Asian bigheaded carp (Hypophthalmichthys spp.), an invasive fish spreading toward Laurentian Great...
Quantitative real‐time PCR (QPCR) is a powerful and sensitive method for quantitative detection of microorganisms. Application this methodology enumeration harmful algal bloom (HAB) species has the potential to revolutionize our approach HAB research, making it possible identify correlations between cell abundances factors that regulate dynamics. Its application ecological studies, however, produced mixed results. QPCR assays typically rely on generation standard curves from plasmids or...
The primary structure of the α-chain preCol-D (molecular mass = 80 kDa), a tanned collagenous protein predominating in distal portion byssal threads mussel Mytilus edulis, was deduced from cDNA to encode an unprecedented natural block copolymer with three major domain types: central collagen flanked by fibroin-like domains and followed histidine-rich termini. have sequence motifs that strongly resemble crystalline polyalanine-rich amorphous glycine-rich regions spider dragline silk fibroins....
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 24:275-285 (2001) - doi:10.3354/ame024275 Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular probing techniques Kathryn J. Coyne, David A. Hutchins, Clinton E. Hare, S. Craig Cary* University of Delaware Graduate College Marine Studies, 700 Pilottown Rd, Lewes, 19958, USA...
Vibrio species are an abundant and diverse group of bacteria that form associations with phytoplankton. Correlations between phytoplankton abundance have been noted, suggesting growth is enhanced during algal blooms or association provides a refuge from predation. Here, we investigated relationships particle-associated spp. in Delaware's inland bays (DIB). The relative abundances classes DIB (dinoflagellates, diatoms, raphidophytes) were determined using quantitative PCR. results...
Abstract Application of algicides produced by naturally occurring bacteria is considered an environmentally friendly approach to control harmful algal blooms. However, few studies assess the effects bacterial on non-target species, either independently or with other stressors. Here, we measured sub-lethal dinoflagellate-specific algicide IRI-160AA estuarine fish Fundulus heteroclitus and Menidia menidia in laboratory experiments. Plasma cortisol levels were test whether a neuroendocrine...
Aureococcus anophagefferens is a small pelagophyte that has caused harmful brown tide blooms in New York, Jersey, Rhode Island, and Maryland. It visually indistinguishable from other algae environmental samples using standard light microscopy, current species‐specific immunofluorescent counting techniques are not sensitive enough to detect concentrations lower than approximately 5000 cells mL −1 . A TaqMan molecular probe was developed specific for A. based on unique 18S rDNA sequence at low...
Abstract Bacteria may play a role in regulating harmful algal blooms, but little is known about the biochemical and physiological changes associated with cell death induced by algicidal bacteria. Previous work characterized an exudate (IRI-160AA) produced Shewanella sp. IRI-160 that effective against dinoflagellates, while having to no effect on other phytoplankton species laboratory culture experiments. The objective of this study was evaluate impacts cycle three dinoflagellate (...
Ammonium and polyamines are essential nitrogen metabolites in all living organisms. Crosstalk between ammonium through their metabolic pathways has been demonstrated plants animals, while no research directed to explore this relationship algae or investigate the underlying molecular mechanisms. Previous that high concentrations of putrescine were among active substances bacteria-derived algicide targeting dinoflagellates, suggesting biochemical inter-connection and/or interaction these...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 40:121-132 (2005) - doi:10.3354/ame040121 Evaluating vertical migration behavior of harmful raphidophytes in Delaware Inland Bays utilizing quantitative real-time PCR S. M. Handy1, K. J. Coyne1, Portune1, E. Demir1, A. Doblin2, C. Hare1, Cary1, D. Hutchins1,* 1University Delaware, Graduate College Marine Studies, 700...
High levels of intraspecific variability are often associated with HAB species, and this is likely an important factor in their competitive success. Heterosigma akashiwo (Hada) Hada ex Y. Hara et M. Chihara ichthyotoxic raphidophyte capable forming dense surface-water blooms temperate coastal regions throughout the world. We isolated four strains H. from fish-killing northern Puget Sound 2006 2007. By assessing numerous aspects biochemistry, physiology, toxicity, we were able to describe...