A5066619822- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Fossil Insects in Amber
- CRISPR and Genetic Engineering
- Financial Crisis of the 21st Century
- Marine and coastal plant biology
- Polar Research and Ecology
- Historical Influence and Diplomacy
- Genetics, Bioinformatics, and Biomedical Research
- Historical Art and Culture Studies
- Lepidoptera: Biology and Taxonomy
- Climate Change Communication and Perception
- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- Bioinformatics and Genomic Networks
- Environmental DNA in Biodiversity Studies
- Plant Parasitism and Resistance
- Visual Culture and Art Theory
- Reformation and Early Modern Christianity
- Art History and Market Analysis
Western University
2016-2025
University of Ottawa
2022
Miami University
2022
University of British Columbia
2011-2020
University of Aberdeen
2018
Nutrition Sciences (Belgium)
2018
Canadian Institute for Advanced Research
2011-2017
United States Fish and Wildlife Service
2017
Smithsonian Institution
2014-2017
Aurora Health Care
2015-2016
Current sampling of genomic sequence data from eukaryotes is relatively poor, biased, and inadequate to address important questions about their biology, evolution, ecology; this Community Page describes a resource 700 transcriptomes marine microbial help understand role in the world's oceans.
The green lineage (Viridiplantae) comprises the algae and their descendants land plants, is one of major groups oxygenic photosynthetic eukaryotes. Current hypotheses posit early divergence two discrete clades from an ancestral flagellate. One clade, Chlorophyta, diverging prasinophytes, which gave rise to core chlorophytes. other Streptophyta, includes charophyte plants evolved. Multi-marker genome scale phylogenetic studies have greatly improved our understanding broad-scale relationships...
Mitochondrial and plastid genomes show a wide array of architectures, varying immensely in size, structure, content. Some organelle DNAs have even developed elaborate eccentricities, such as scrambled coding regions, nonstandard genetic codes, convoluted modes posttranscriptional modification editing. Here, we compare contrast the breadth genomic complexity between mitochondrial chromosomes. Both independently evolved many same features taken on similar embellishments, often within species...
The transition to multicellularity has occurred numerous times in all domains of life, yet its initial steps are poorly understood. volvocine green algae a tractable system for understanding the genetic basis including formation cooperative cell groups. Here we report genome sequence undifferentiated colonial alga, Gonium pectorale, where group evolved by co-option retinoblastoma cycle regulatory pathway. Significantly, expression regulator unicellular Chlamydomonas causes it become...
Polytomella spp. are free-living, nonphotosynthetic green algae closely related to the model organism Chlamydomonas reinhardtii. Although colorless, have a plastid, but it is still unknown whether they harbor plastid genome. We took next generation sequencing approach, along with transcriptome sequencing, search for genome and an associated gene expression system in Illumina of total DNA from four did not produce any recognizable plastid-derived reads generate large number mitochondrial...
Dunaliella salina Teodoresco, a unicellular, halophilic green alga belonging to the Chlorophyceae, is among most industrially important microalgae. This because D. can produce massive amounts of β-carotene, which be collected for commercial purposes, and its potential as feedstock biofuels production. Although biochemistry physiology have been studied in great detail, virtually nothing known about genomes it carries, especially those within mitochondrion plastid. study presents complete...
The abundance of nuclear plastid DNA-like sequences (NUPTs) in genomes can vary immensely; however, the forces responsible for this variation are poorly understood. "The limited transfer window hypothesis" predicts that species with only one per cell will have fewer NUPTs than those many plastids cell, but a lack genome sequence data from monoplastidic has made hypothesis difficult to test. Here, by analyzing newly available diverse mono- and polyplastidic taxa, we show holds. On average,...
Mitochondrial genomes often contain large amounts of plastid DNA (ptDNA)-derived sequences (MTPTs).It has been suggested that the intercompartmental transfer ptDNA is greatly reduced in species with only a single per cell (monoplastidic) as compared those many plastids (polyplastidic).This hypothesis not applied to movement from mitochondria.By analyzing organelle diverse mono-and polyplastidic taxa, I show MTPTs are restricted mitochondrial and absent one or monoplastidic meristematic...
The magnitude of noncoding DNA in organelle genomes can vary significantly; it is argued that much this variation attributable to the dissemination selfish DNA. results a previous study indicate mitochondrial (mtDNA) green alga Volvox carteri abounds with palindromic repeats, which appear be elements. We became interested evolution and distribution these repeats when, during cursory exploration V. nuclear (nucDNA) plastid (ptDNA) sequences, we found similar structural features those mtDNA....
Animal mitochondrial DNAs (mtDNAs) are typically single circular chromosomes, with the exception of those from medusozoan cnidarians (jellyfish and hydroids), which linear sometimes fragmented. Most medusozoans have monomeric or bipartite genomes, but preliminary data suggested that box jellyfish (cubozoans) mtDNAs consist many chromosomes. Here, we present complete mtDNA sequence winged Alatina moseri (the first a cubozoan). This genome contains unprecedented levels fragmentation: 18 unique...
Organelle genomes show remarkable variation in architecture and coding content, yet their nucleotide composition is relatively unvarying across the eukaryotic domain, with most having a high adenine thymine (AT) content. Recent studies, however, have uncovered guanine cytosine (GC)-rich mitochondrial plastid genomes. These sequences come from small but eclectic list of species, including certain green plants animals. Here, I review GC-rich organelle DNAs insights they provided into evolution...
Microsporidia from the Encephalitozoonidae are obligate intracellular parasites with highly conserved and compacted nuclear genomes: they have few introns, short intergenic regions, almost identical gene complements chromosome arrangements. Comparative genomics of Encephalitozoon microsporidia in general focused largely on genomic diversity between different species, we know very little about levels genetic within species. Polymorphism studies so far restricted to a small number genes,...
It has been argued that for certain lineages, noncoding DNA expansion is a consequence of the increased random genetic drift associated with long-term escalations in organism size. But lack data prevented investigation this hypothesis most plastid-bearing protists. Here, using newly sequenced mitochondrial and plastid genomes, we explore relationship between organelle content size within volvocine green algae. By looking at unicellular, colonial, differentiated multicellular algae, show...
Why is there such a large variation in size and noncoding DNA content among organelle genomes? One explanation that this genomic results from differences the rates of mutation random genetic drift, as opposed to being direct product natural selection. Along these lines, mutational hazard hypothesis (MHH) holds 'excess' liability (because it increases potential for harmful mutations) and, thus, has greater tendency accumulate an system with low rate one high mutation. Various studies have...
Advancements in high-throughput nucleotide sequencing techniques have brought with them state-of-the-art bioinformatics programs and software packages. Given the importance of molecular sequence data contemporary life science research, these suites are becoming an essential component many labs classrooms, as such frequently designed for non-computer specialists marketed one-stop toolkits. Although beautifully powerful, user-friendly packages can be expensive and, more arrive on market each...
Antarctica is home to an assortment of psychrophilic algae, which have evolved various survival strategies for coping with their frigid environments. Here, we explore Antarctic psychrophily by examining the ∼212 Mb draft nuclear genome green alga Chlamydomonas sp. UWO241, resides within water column a perennially ice-covered, hypersaline lake. Like certain other UWO241 encodes large number (≥37) ice-binding proteins, putatively originating from horizontal gene transfer. Even more striking,...
One common observation concerning mitochondrial genomes is that they have a low guanine and cytosine content (GC content); of the complete genome sequences currently available at National Center for Biotechnology Information (NCBI) (July 2007), GC ranges from 13.3% to 53.2% has an average value 38%. Here, we present GC-rich (57% GC) colorless green alga Polytomella capuana. The disproportion among different regions P. capuana DNA (mtDNA) suggests neutral process responsible bias. We propose...
The noncoding-DNA content of organelle and nuclear genomes can vary immensely. Both adaptive nonadaptive explanations for this variation have been proposed. This study addresses a explanation called the mutational-hazard hypothesis applies it to mitochondrial, plastid, multicellular green alga Volvox carteri. Given expanded architecture V. carteri (60-85% noncoding DNA), would predict them less silent-site nucleotide diversity (π(silent)) than their more compact counterparts from other...