A5081385223- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Protist diversity and phylogeny
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Insects and Parasite Interactions
- Chromosomal and Genetic Variations
- Neurobiology and Insect Physiology Research
- Machine Learning in Bioinformatics
- Microbial Community Ecology and Physiology
- Nematode management and characterization studies
- Myxozoan Parasites in Aquatic Species
- Protein Kinase Regulation and GTPase Signaling
Leipzig University
2019-2024
Stanford Medicine
2023
University of Copenhagen
2023
Tsinghua University
2023
Institute of Biophysics
2023
Abstract With the rapid increase of sequenced metazoan mitochondrial genomes, a detailed manual annotation is becoming more and infeasible. While it easy to identify approximate location protein-coding genes within mitogenomes, peculiar processing transcripts, however, makes determination precise gene boundaries surprisingly difficult problem. We have analyzed properties annotated start stop codon positions in detail, use inferred patterns devise new method for predicting de novo...
The Neuropeptide Y/RFamide-like receptors belong to the Rhodopsin-like G protein-coupled (GPCRs) and are involved in functions such as locomotion, feeding reproduction. With 41 described they form best-studied group of neuropeptide GPCRs Caenorhabditis elegans. In order understand expansion receptor family nematodes, we started from sequences selected paralogs C. elegans query surveyed corresponding orthologous another 159 representative nematode target genomes. To this end employed a...
Abstract Mitochondrial genomes of apicomplexans, dinoflagellates, and chrompodellids that collectively make up the Myzozoa, encode only three proteins (Cytochrome b [COB], Cytochrome c oxidase subunit 1 [COX1], 3 [COX3]), contain fragmented ribosomal RNAs, display extensive recombination, RNA trans-splicing, RNA-editing. The early-diverging Perkinsozoa is final major myzozoan lineage whose mitochondrial remained poorly characterized. Previous reports Perkinsus genes indicated independent...
Abstract In tRNA maturation, CCA-addition by nucleotidyltransferase is a unique and highly accurate reaction. While the mechanism of nucleotide selection polymerization well understood, it remains mystery why bacterial eukaryotic enzymes exhibit an unexpected surprisingly low substrate affinity while they efficiently catalyze CCA-addition. To get insights into evolution this high-fidelity RNA synthesis, reconstruction characterization ancestral versatile tool. Here, we investigate...
Spliced alignments are a key step in the construction of high-quality homology-based annotations protein sequences. The exon/intron structure, which is computed as part spliced alignment procedures, often conveys important information for distinguishing paralogous members gene families. Here we present an exon-centric pipeline that intended particular applications involve exon-by-exon comparisons coding We show simple, blat-based approach has advantages over established tools genes with very...
<title>Abstract</title> G protein-coupled receptors (GPCRs) activate heterotrimeric proteins by promoting guanine nucleotide exchange. Here, we investigate the process of functional association between and GPCRs describe events that ultimately lead to ejection GDP from its binding pocket in Gα subunit. In atomic detail, reveal temporal progression structural rearrangements GDP-bound Gs protein (GsGDP) upon coupling β2-adrenergic receptor (β2AR) using molecular dynamics simulations. The GsGDP...
ABSTRACT Mitochondrial genomes of apicomplexans, dino-flagellates and chrompodellids, that collectively make up the Myzozoa, are uncommonly reduced in coding capacity display divergent gene configuration expression mechanisms. They encode only three proteins — COB, COX1, COX3 contain rRNAs fragmented to ∼100-200 base pair elements, employ extensive recombination, RNA trans-splicing, RNA-editing for genome maintenance expression. The early-diverging Perkinsozoa is final major myzozoan lineage...