A5078035156- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Copper Interconnects and Reliability
- Cancer-related molecular mechanisms research
- Protist diversity and phylogeny
- Biochemical and Molecular Research
- Probiotics and Fermented Foods
- Metabolism and Genetic Disorders
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Identification and Quantification in Food
- Plant and Fungal Interactions Research
- Polymer composites and self-healing
Leipzig University
2016-2025
University of Bayreuth
2017
Max Planck Institute for Mathematics
2012
University of Vienna
2012
Fraunhofer Institute for Cell Therapy and Immunology
2012
Max Planck Society
1998-2005
Max Planck Institute for Evolutionary Anthropology
1998-2005
Bavarian State Collection of Zoology
1995-1997
Ludwig-Maximilians-Universität München
1990-1995
Technische Universität Berlin
1990
One of the first specialized collections nucleic acid sequences in life sciences was 'compilation tRNA and genes' (http://www.trna.uni-bayreuth.de). Here, an updated completely restructured version this compilation is presented (http://trnadb.bioinf.uni-leipzig.de). The new database, tRNAdb, hosted maintained cooperation between universities Leipzig, Marburg, Strasbourg. Reimplemented as a relational tRNAdb will be periodically searchable highly flexible user-friendly way. Currently, it...
SELEX is an iterative process in which highly diverse synthetic nucleic acid libraries are selected over many rounds to finally identify aptamers with desired properties. However, little understood as how binders enriched during the selection course. Next-generation sequencing offers opportunity open black box and observe a large part of population dynamics process.We have performed semi-automated procedure on model target streptavidin starting DNA oligonucleotide library compared results...
Riboswitches are regulatory RNA elements typically located in the 5′-untranslated region of certain mRNAs and control gene expression at level transcription or translation. These consist a sensor an adjacent actuator domain. The usually is aptamer that specifically interacts with ligand. contains intrinsic terminator ribosomal binding site for transcriptional translational regulation, respectively. Ligand leads to structural rearrangements riboswitch presentation masking these elements....
Mass spectrometry (MS)-based quantitative interaction proteomics has successfully elucidated specific protein-protein, DNA-protein, and small molecule-protein interactions. Here, we developed a gel-free, sensitive, scalable technology that addresses the important area of RNA-protein Using aptamer-tagged RNA as bait, captured RNA-interacting proteins from stable isotope labeling by amino acids in cell culture (SILAC)-labeled mammalian extracts analyzed them high-resolution, MS. Binders to...
The pyrrolysyl-tRNA synthetase/tRNAPyl pair is the most versatile and widespread system for incorporation of non-canonical amino acids (ncAAs) into proteins in mammalian cells. However, low yields ncAA severely limit its applicability to relevant biological targets. Here, we generate two tRNAPyl variants that significantly boost performance pyrrolysine system. Compared original tRNAPyl, engineered tRNAs feature a canonical hinge between D- T-loop, show higher intracellular concentrations...
Stress-induced changes of gene expression are crucial for survival eukaryotic cells. Regulation at the level translation provides necessary plasticity immediate cellular activities and protein levels. In this study, we demonstrate that exposure to oxidative stress results in a quick repression by deactivation aminoacyl-ends all transfer-RNA (tRNA). An oxidative-stress activated nuclease, angiogenin, cleaves first within conserved single-stranded 3′-CCA termini tRNAs, thereby blocking their...
Riboswitches have gained attention as tools for synthetic biology, since they enable researchers to reprogram cells sense and respond exogenous molecules. In vitro evolutionary approaches produced numerous RNA aptamers that bind such small ligands, but their conversion into functional riboswitches remains difficult. We previously developed a computational approach the design of theophylline based on secondary structure prediction. These been constructed regulate ligand-dependent...
Many repetitive DNA elements are transcribed at appreciable expression levels. Mapping the corresponding RNA sequencing reads back to a reference genome is notoriously difficult and error-prone task, however. This in particular true if chemical modifications introduce systematic mismatches, while same time genomic loci only approximately identical, as case of tRNAs.We therefore developed dedicated mapping strategy handle RNA-seq that map tRNAs relying on modified target which known tRNA...
tRNA nucleotidyltransferase represents a ubiquitous and essential activity that adds the indispensable CCA triplet to 3'-end of tRNAs. To fulfill this function, enzyme contains set highly conserved motifs whose coordinated interplay is crucial for sequence-specific polymerization. In human enzyme, alterations within these regions have been shown lead manifestation disease. Recently, we developed an in vivo screening system allows selection analysis variants by challenging terminal AMP...
Playing a central role in translation, tRNAs act as an essential adapter linking the correct amino acid to corresponding mRNA codon translation. Due this function, all exhibit typical secondary and tertiary structure be recognized by tRNA maturation enzymes well many components of translation machinery. Yet, there is growing evidence for structurally deviating metazoan mitochondria, requiring co-evolution adaptation these unusual structures their substrates. Here, it shown that CCA-adding...
Riboswitches are RNA-based regulators of gene expression composed a ligand-sensing aptamer domain followed by an overlapping platform. The regulation occurs at either the level transcription (by formation terminator or antiterminator structures) translation presentation sequestering ribosomal binding site). Due to modular composition, these elements can be manipulated combining different aptamers and platforms therefore represent useful tools regulate in synthetic biology. Using...
As adapter molecules to convert the nucleic acid information into amino sequence, tRNAs play a central role in protein synthesis. To fulfill this function reliable way, exhibit highly conserved structural features common all organisms and cellular compartments active translation. However, mitochondria of metazoans, certain dramatic deviations from consensus tRNA structure are described, where some lack D- or T-arm without losing their function. In Enoplea, miniaturization comes an extreme,...
Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, microfluidic device described that enables by counter-diffusion and their direct on-chip analysis serial crystallography at room temperature. Nine `non-model' diverse biomacromolecules, including seven soluble proteins, membrane protein an RNA duplex, were crystallized treated with variety standard techniques micro-seeding, crystal soaking ligands detection...
The mitochondrial tRNA gene for lysine was analyzed in 11 different marsupial mammals. Whereas its location is conserved when compared with other vertebrate genomes, primary sequence and inferred secondary structure are highly unusual variable. For example, eight species lack the expected anticodon. Because corresponding transcripts not altered by any RNA-editing mechanism, lysyl-tRNA seems to represent a pseudogene. Purification of mitochondria vitro aminoacylation isolated tRNAs lysine,...