A5083891437- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Systemic Lupus Erythematosus Research
- Protein purification and stability
- Advanced biosensing and bioanalysis techniques
- Gene expression and cancer classification
- Cytokine Signaling Pathways and Interactions
- Bacteriophages and microbial interactions
- Advanced Proteomics Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Rheumatoid Arthritis Research and Therapies
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Osteoarthritis Treatment and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Research on Leishmaniasis Studies
- Mass Spectrometry Techniques and Applications
Federal Institute For Materials Research and Testing
2020-2025
Max Planck Institute for Molecular Genetics
2008-2021
Max Planck Institute of Colloids and Interfaces
2013-2019
Max Planck Society
2003-2017
Ruhr University Bochum
2004
Royal College of Surgeons in Ireland
2003-2004
SELEX is an iterative process in which highly diverse synthetic nucleic acid libraries are selected over many rounds to finally identify aptamers with desired properties. However, little understood as how binders enriched during the selection course. Next-generation sequencing offers opportunity open black box and observe a large part of population dynamics process.We have performed semi-automated procedure on model target streptavidin starting DNA oligonucleotide library compared results...
Guanine quadruplex (G-quadruplex) motifs in the 5' untranslated region (5'-UTR) of mRNAs were recently shown to influence efficiency translation. In present study, we investigate interaction between cellular proteins and G-quadruplexes located two (MMP16 ARPC2). Formation was confirmed by biophysical characterization inhibitory activity on translation luciferase reporter assays. experiments with whole cell extracts from different eukaryotic lines, G-quadruplex-binding isolated pull-down...
In recent years the improvements in high-throughput gene expression analysis have led to discovery of numerous non-protein-coding RNA (npcRNA) molecules. They form an abundant class untranslated RNAs that shown play a crucial role different biochemical pathways cell. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is efficient tool measure abundance and levels tiny amounts material. Despite its sensitivity, lack appropriate internal controls necessary for...
The enzyme-linked immunosorbent assay (ELISA) is typically applied in the format of microtiter plates. To increase throughput and reduce consumption precious samples, efforts have been made to transfer ELISA microchip using conventional microarrays, microfluidic systems, chips bearing microwells. However, all three formats lack possibility screen several analytes on immobilized binders at a time or require complicated liquid handling, surface modifications, additional equipment. Here, we...
Secretory signal peptides (SPs) are well-known sequence motifs targeting proteins for translocation across the endoplasmic reticulum membrane. After passing through secretory pathway, most secreted to environment. Here, we describe modification of an expression vector containing SP from acid phosphatase 1 (SAP1) Leishmania mexicana optimized protein expression-secretion in eukaryotic parasite tarentolae with regard recombinant antibody fragments. For experimental design online tool SignalP...
Prader-Willi Syndrome (PWS) is a neurogenetic disorder caused by the deletion of imprinted genes on paternally inherited human chromosome 15q11-q13. This locus harbours long non-protein-coding RNA (U-UBE3A-ATS) that contains six intron-encoded snoRNAs, including SNORD116 and SNORD115 repetitive clusters. The 3′-region U-UBE3A-ATS transcribed in cis-antisense direction to ubiquitin-protein ligase E3A (UBE3A) gene. Deletion region causes key characteristics PWS. There are few indications might...
Porphyromonas gingivalis (P.g.) is discussed to be involved in triggering self-reactive immune responses. The aim of this study was investigate the autocitrullinated prokaryotic peptidylarginine deiminase (PPAD) from P.g. CH2007 (RACH2007-PPAD) a rheumatoid arthritis (RA) patient and synthetic citrullinated PPAD peptide (CPP) containing main autocitrullination site as potential targets for antibody reactivity RA analyse possibility citrullinating native human proteins by context...
We describe a high-throughput, quantitative technology for fast identification of all different clones present in selectively enriched phage surface-displayed cDNA libraries. The strategy is based on combination display and high-density arrays. To demonstrate the utility method cDNAs Aspergillus fumigatus cloned into phagemid pJuFo were expressed tip filamentous M13 affinityselected solid phase-immobilized serum IgE from allergic patients. Enriched libraries amplified bacteria, plated...
Protein affinity reagents (PARs), most commonly antibodies, are essential for protein characterization in basic research, biotechnology, and diagnostics as well the fastest growing class of therapeutics. Large numbers PARs available commercially; however, their quality is often uncertain. In addition, currently cover only a fraction human proteome, cost prohibitive proteome scale applications. This situation has triggered several initiatives involving large generation validation example...
Gene transcription is controlled by transcriptional regulators acting with specific co-regulators to allow gene activation and repression. Here, we report the identification of KRAB-containing zinc-finger regulator, ZBRK1, as an interaction partner SCA2 product ataxin-2. Furthermore, discovered that elevated ZBRK1 level resulted in increased ataxin-2 levels, whereas interference on protein levels yielded reduced suggesting a complex comprising regulates transcription. A bioinformatic...
The immune system protects us from foreign substances or pathogens by generating specific antibodies. variety of immunoglobulin (Ig) paratopes for antigen recognition is a result the V(D)J rearrangement mechanism, while fast and efficient response mediated isotypes obtained through class switch recombination (CSR). To get better understanding on how antibody-based protection works it changes with age, interdependency between these two parameters need to be addressed. Here, we have performed...
A growing body of evidence suggests a role for soluble alpha-amyloid precursor protein (sAPPalpha) in pathomechanisms Alzheimer disease (AD). This cleavage product APP was identified to have neurotrophic properties. However, it remained enigmatic what proteins, targeted by sAPPalpha, might be involved such neuroprotective actions. Here, we used high-resolution two-dimensional polyacrylamide gel electrophoresis analyze proteome changes downstream sAPPalpha neurons. We present that regulates...
Automation is the key approach for genomewide and proteomewide screening of function interaction. Especially proteomics, antibody microarrays are a useful tool massive parallel profiling complex samples. To meet requirements to obtain great variety antibodies, new technologies such as phage display have partly replaced classical hybridoma method. While selection process phage-displayed fragments itself has been automated, bottleneck was shifted further downstream identification monoclonal...