A5052707025- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Photosynthetic Processes and Mechanisms
- 3D Printing in Biomedical Research
- Viral gastroenteritis research and epidemiology
- Spectroscopy and Quantum Chemical Studies
- RNA modifications and cancer
- Additive Manufacturing and 3D Printing Technologies
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Pain Mechanisms and Treatments
- Animal Virus Infections Studies
- Ion Channels and Receptors
- CAR-T cell therapy research
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Viral Infectious Diseases and Gene Expression in Insects
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biochemical and Molecular Research
- Bone Tissue Engineering Materials
- MicroRNA in disease regulation
Technische Universität Berlin
2016-2025
Czech Academy of Sciences, Institute of Biotechnology
2014-2025
National Centre for the Replacement Refinement and Reduction of Animals in Research
2022
Freie Universität Berlin
2001-2011
University of Stuttgart
2008-2010
Charité - Universitätsmedizin Berlin
2008-2009
Medical University of Graz
2009
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2008
Russian Academy of Sciences
1999
Institute of Basic Biological Problems
1999
The design of antisense oligonucleotides containing locked nucleic acids (LNA) was optimized and compared to intensively studied DNA oligonucleotides, phosphorothioates 2′-O-methyl gapmers. In contradiction the literature, a stretch seven or eight monomers in center chimeric DNA/LNA oligonucleotide is necessary for full activation RNase H cleave target RNA. For gapmers six sufficient recruit H. Compared 18mer LNA have an increased melting temperature 1.5–4°C per depending on positions...
Activation of the hypoxia-inducible factor alpha-subunits, HIF-1alpha and HIF-2alpha, seems to be subject similar regulatory mechanisms, transgene approaches suggested partial functional redundancy. Here, we used RNA interference determine contribution vs. HIF-2alpha hypoxic gene induction. Surprisingly, most genes tested were responsive only siRNA, showing no effect by knock-down. The same was found for activation reporter driven hypoxia-responsive elements (HREs) from erythropoietin (EPO),...
Locked nucleic acids (LNAs) and double-stranded small interfering RNAs (siRNAs) are rather new promising antisense molecules for cell culture in vivo applications. Here, we compare LNA-DNA-LNA gapmer oligonucleotides siRNAs with a phosphorothioate chimeric 2'-O-methyl RNA-DNA respect to their capacities knock down the expression of vanilloid receptor subtype 1 (VR1). gapmers four or five LNAs on either side central stretch 10 8 DNA monomers center were found be active that inhibit gene...
Guanine-rich sequences can adopt intramolecular four-stranded structures, called G-quadruplexes. These motifs have been intensively investigated on the DNA level, but their overall biological relevance remains elusive. Only recently has research concerning function of G-quadruplexes in RNAs commenced. Here, we demonstrate for first time, that an RNA G-quadruplex structure inhibits translation vivo eukaryotic cells. We a highly conserved, thermodynamically stable 5′-UTR mRNA human Zic-1...
Background— RNA interference (RNAi) has the potential to be a novel therapeutic strategy in diverse areas of medicine. Here, we report on targeted RNAi for treatment heart failure, an important disorder humans that results from multiple causes. Successful failure is demonstrated rat model transaortic banding by targeting phospholamban, key regulator cardiac Ca 2+ homeostasis. Whereas gene therapy rests recombinant protein expression as its basic principle, uses regulatory RNAs achieve...
Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of bioink composition extrusion printing. bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting alginate, gelatin Matrigel used provide scaffold 3D arrangement human alveolar A549 cells. A blend containing...
Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed human cells. The present study describes optimization bioink alginate, gelatin extracellular matrix (hECM) print HepaRG liver cells pneumatic extrusion printer. resulting model was tested for its suitability transduction by an adeno-associated virus (AAV) vector infection...
Guanine quadruplex (G-quadruplex) motifs in the 5' untranslated region (5'-UTR) of mRNAs were recently shown to influence efficiency translation. In present study, we investigate interaction between cellular proteins and G-quadruplexes located two (MMP16 ARPC2). Formation was confirmed by biophysical characterization inhibitory activity on translation luciferase reporter assays. experiments with whole cell extracts from different eukaryotic lines, G-quadruplex-binding isolated pull-down...
Effective communication is crucial for broad acceptance and applicability of alternative methods in 3R biomedical research preclinical testing. 3D bioprinting used to construct intricate biological structures towards functional liver models, specifically engineered deployment as models drug screening, toxicological investigations, tissue engineering. Despite a growing number reviews this emerging field, comprehensive study, systematically assessing practices reporting quality bioprinted missing.
The inclusion of small amounts copper is often reported to enhance the mechanical and biointegrative performance bioceramics towards tissue engineering applications. In this work, 3D scaffolds were additively manufactured by robocasting precipitation derived doped diopside. Compositions chosen in which magnesium sites diopside substituted up 3 at.%. Microstructure, performance, bioactivity, biodegradability, drug release, biocompatibility, vitro angiogenesis antibacterial activity studied....
Abstract 3D bioprinting enables the fabrication of human organ models that can be used for various fields biomedical research, including oncology and infection biology. An important challenge, however, remains generation vascularized, perfusable closely simulate natural physiology. Here, a novel direct ink writing (DIW) approach is described produce vascularized without using sacrificial materials during fabrication. The high resolution method allows one‐step sophisticated hollow geometries....
Three-dimensional (3D) bioprinting is one of the most promising methodologies that are currently in development for replacement animal experiments. Bioprinting and alternative technologies rely on animal-derived materials, which compromises intent welfare results generation chimeric systems limited value. The current study therefore presents first bioprinted liver model entirely void constituents. Initially, HuH-7 cells underwent adaptation to a chemically defined medium (CDM). adapted...
Nucleoside and nucleotide analogues have proven to be transformative in the treatment of viral infections cancer. One branch structural modification deliver new nucleoside analogue classes explores replacement canonical ribose oxygen with a sulfur atom. Whilst biological activity such has been shown some cases, widespread exploration this compound class is hitherto hampered by lack straightforward universal nucleobase diversification strategy. Herein, we present synergistic platform enabling...
A systematic mutagenesis study of the "10-23" DNA enzyme was performed to analyze sequence requirements its catalytic domain. Therefore, each 15 core nucleotides substituted separately by remaining three naturally occurring nucleotides. Changes at borders domain led a dramatic loss enzymatic activity, whereas several in between could be exchanged without severe effects. Thymidine position 8 had lowest degree conservation and substitution any other caused only minor activity. In addition...