A5028526388- Contact Dermatitis and Allergies
- DNA Repair Mechanisms
- Dermatology and Skin Diseases
- Occupational exposure and asthma
- Allergic Rhinitis and Sensitization
- Tattoo and Body Piercing Complications
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Body Image and Dysmorphia Studies
- RNA Interference and Gene Delivery
- Carcinogens and Genotoxicity Assessment
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Drug-Induced Adverse Reactions
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Pesticide Exposure and Toxicity
- Virus-based gene therapy research
- Porphyrin Metabolism and Disorders
- Genetic factors in colorectal cancer
- Cancer, Lipids, and Metabolism
- Historical Medical Research and Treatments
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
University of Göttingen
2016-2025
Universitätsmedizin Göttingen
2015-2025
Silence Therapeutics (Germany)
2018-2024
Cenix BioScience (Germany)
2013-2020
Universitätsklinikum Würzburg
2017-2020
Ludwig-Maximilians-Universität München
2013-2016
Verbundzentrale des GBV
2015
Harvard University
2010-2013
First Pavlov State Medical University of St. Petersburg
2013
Emory University
2013
'10-23' DNAzymes can be used to cleave any target RNA in a sequence-specific manner. For applications vivo, they have stabilised against nucleolytic attack by the introduction of modified nucleotides without obstructing cleavage activity. In this study, we optimise design DNAzyme targeting 5'-non-translated region human rhinovirus 14, common cold virus, with regard its kinetic properties and stability nucleases. We compare large number same site that are use 3'-3'-inverted thymidine,...
Continual analyses of patch test results with the European baseline series (EBS) serve both contact allergy surveillance and auditing value included allergens.To present current EBS testing, obtained in 53 departments 13 countries during 2019 2020.Anonymised or pseudonymised individual data partly aggregated on demographic/clinical characteristics rest were prospectively collected centrally pooled analysed.In 2020, 22 581 patients tested EBS. Sensitization to nickel remained most common...
Glial cells missing a (GCMa) belongs to new transcription factor family. Syncytin was shown be target gene of GCMa. Here, we demonstrate that the protein kinase A (PKA) pathway acts upstream After transient transfection BeWo with PKA, GCMa transcriptional activity and both syncytin transcripts were upregulated. This increase accompanied by further cellular differentiation. Using normoxic or hypoxic conditions mimic pathophysiological settings known diminish trophoblast differentiation, found...
In 2019, a number of allergens (haptens), henceforth, "the audit allergens," were considered as potential additions to the European Baseline Series (EBS), namely, sodium metabisulfite, 2-bromo-2-nitropropane-1,3-diol, diazolidinyl urea, imidazolidinyl Compositae mix II (2.5% or 5% pet), linalool hydroperoxides (lin-OOH), limonene (lim-OOH), benzisothiazolinone (BIT), octylisothiazolinone (OIT), decyl glucoside, and lauryl glucoside; Evernia furfuracea (tree moss), was additionally tested by...
The majority of mammalian microRNA (miRNA) genes reside within introns protein-encoding and non-coding genes, yet the mechanisms coordinating primary transcript processing into both mature miRNA spliced mRNA are poorly understood. Analysis melanoma invasion suppressor miR-211 expressed from intron 6 melastatin revealed that microprocessing promotes splicing exon 6–exon 7 junction by a mechanism requiring RNase III activity Drosha. Additionally, mutations in 5′ splice site (5′SS), but not...
ABSTRACT Background Since its inception in 1980, the MOHL index (% patients who are male, have occupational, hand, or leg dermatitis, respectively) and later evolutions until presently used MOAHLFA(P) (adding % with atopic face age 40+ years positive reaction(s) to ≥ 1 baseline series allergen) been intended convey important demographic clinical information on patch tested a certain area time, aiding interpretation of observed spectrum sensitisation. Objectives To examine current usage...
Isolated hypoparathyroidism is an uncommon metabolic disorder characterized by hypocalcemia and hyperphosphatemia, with absent or low levels of PTH. It may present as apparently sporadic be transmitted in families a genetic trait. Mutations the calcium-sensing receptor gene preproPTH have been reported occasional cases, mutation parathyroid-specific transcription factor GCMB has one familial case. We report second family isolated mutation. The patients were two siblings from asymptomatic,...
Summary Background Dental technicians (DTs) are at increased risk for allergic contact sensitization. Objectives To assess the current spectrum of occupational sensitization in DTs with dermatitis (OCD). Methods A retrospective analysis Information Network Departments Dermatology patch test data from years 2001–2015 concerning OCD was performed. Results Patients study group (226 OCD) were significantly more often diagnosed (37.6% versus 18.5%; p = 0.0002) than patients control (124 without...
Sensitization to methylisothiazolinone (MI) has seen an exceptional epidemic, mainly attributed its use in cosmetics.To trace the epidemic of MI allergy (2009-2018), and analyze a possible change patients' characteristics.Informationsverbund Dermatologischer Kliniken-data patients patch tested between 2009 2018 with (0.05% aq.) were analyzed concerning anamnestic items sensitization frequencies.Overall, 4.9% reacted positive MI. Comparing three periods (2009, 2013/14, 2017/18), there was...
Benzisothiazolinone (BIT; CAS no. 2634-33-5) is used as a biocide in various products, including water-based paints, metalworking fluids, and household products. In recent years, increasing sensitization rates have been observed Europe.To describe time trend of to BIT, analyse concomitant reactions, identify patients with increased risk BIT sensitization.Retrospective analysis data from 26 739 patch tested sodium salt, 0.1% petrolatum part several special test series within the Information...
In this study, the functional relevance of core nucleotides RNA cleaving 10-23 DNA enzyme (DNAzyme) was investigated. Systematic deletion studies revealed that DNAzymes lacking thymine at position 8 (T8) retain catalytic activity comparable to wild-type enzyme. Deletion adjacent cytosine 7 (C7) also resulted in a highly active and even double mutant C7/T8 displayed cleavage activity, although rate under multiple turnover conditions found be reduced by one order magnitude. The identification...
The release of Agrin by motoneurons activates the muscle-specific receptor tyrosine kinase (MuSK) as main organizer subsynaptic specializations at neuromuscular junction. MuSK downstream signaling is largely undefined. Here we show that protein CK2 interacts and colocalizes with post-synaptic specializations. We observed CK2-mediated phosphorylation serine residues within insert (KI) MuSK. Inhibition or knockdown CK2, exchange phosphorylatable serines alanines KI MuSK, impaired acetylcholine...
Summary Xeroderma pigmentosum (XP) is an autosomal recessive disease, caused by a gene defect in the nucleotide‐excision‐repair (NER) pathway or translesional DNA synthesis. At age of eight, patients already develop their first skin cancers due to this repair defect. In contrast, Caucasian population tumor formation UV exposed regions occurs at mean 60. The clinical picture among suffering from XP highly diverse and includes signs accelerated aging, UV‐induced cancers, as well ophthalmologic...
Abstract Background Propolis types differ regarding their chemical composition. Objectives To compare patch test results based on Brazilian (Green) propolis with data Chinese (poplar‐type) propolis, and to evaluate the specifications of raw materials used for PT preparations. Methods In Information Network Departments Dermatology (IVDK), 1290 consecutive patients were tested (NH400, SmartPractice Europe). Patch reactivity was compared obtained (NA71, Europe) by calculating frequencies...
Abstract Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections available date, we and others have recently started develop interference (RNAi) approaches prevent propagation. Here describe our strategy development efficient small interfering RNAs (siRNAs) against genomes. Initially, fusion constructs reporter (green fluorescent protein) subgenomic fragments were employed...