A5029099340- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Osteoarthritis Treatment and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Mesenchymal stem cell research
- Autoimmune and Inflammatory Disorders Research
- Advanced Biosensing Techniques and Applications
- Spondyloarthritis Studies and Treatments
- Immunodeficiency and Autoimmune Disorders
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Gene expression and cancer classification
- Periodontal Regeneration and Treatments
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- interferon and immune responses
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Inflammatory Myopathies and Dermatomyositis
- Glycosylation and Glycoproteins Research
Humboldt-Universität zu Berlin
2012-2023
Charité - Universitätsmedizin Berlin
2014-2023
Freie Universität Berlin
2004-2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020
German Rheumatism Research Centre
1999-2019
Center for Rheumatology
2008-2018
Inserm
2018
Berlin-Brandenburger Centrum für Regenerative Therapien
2009-2012
Medical Technologies (Czechia)
2009
Immanuel Krankenhaus
2009
Adult human mesenchymal stromal cells (hMSCs) have the potential to differentiate into chondrogenic, adipogenic, or osteogenic lineages, providing a source for tissue regeneration. An important issue efficient bone regeneration is identify factors that can be targeted promote of hMSCs. Using transcriptome analysis, we found integrin α5 (ITGA5) expression up-regulated during dexamethasone-induced osteoblast differentiation Gain-of-function studies showed ITGA5 promotes phenotypic markers and...
We previously identified multipotent stem cells within the lamina propria of human olfactory mucosa, located in nasal cavity. also demonstrated that this cell type differentiates into neural and improves locomotor behavior after transplantation a rat model Parkinson's disease. Yet, next to nothing is known about their specific stemness characteristics. therefore devised study aiming compare from 4 individuals bone marrow mesenchymal age- gender-matched individuals. Using pangenomic...
Abstract Objective Anti–citrullinated protein antibodies (ACPA) exhibit unique specificity for rheumatoid arthritis. However, it is incompletely understood whether and how ACPA contribute to disease pathogenesis. The Fc part of human IgG carries 2 N‐linked glycan moieties that are crucial the structural stability antibody modulate both its binding affinity Fcγ receptors ability activate complement. We undertook this study analyze glycosylation IgG1 in serum synovial fluid (SF) order further...
Abstract Previous studies have reported that mesenchymal stem cells (MSC) may be isolated from the synovial membrane by same protocol as used for fibroblast cultivation, suggesting MSC correspond to a subset of adherent cell population, stromal compartment bone marrow (BM). The aims present study were, first, better characterize derived and, second, compare systematically, in parallel, MSC-containing populations BM and those synovium, using quantitative assays. Fluorescent-activated sorting...
Abstract Objective. To document the persistence of Borrelia burgdorferi in ligamentous tissue samples obtained from a woman with chronic Lyme borreliosis. Methods. Spirochetes were isolated tissue, and spirochetes characterized antigenetically by molecular biology techniques. The was examined electron microscopy. Humoral cellular immune responses analyzed. Results. Choroiditis first recognized manifestation disease this patient. Despite antibiotic therapy, there progression to stage,...
Abstract Lineage-priming is a molecular model of stem cell (SC) differentiation in which proliferating SCs express subset genes associated to the pathways they can commit. This concept has been developed for hematopoietic SCs, but poorly studied other SC populations. Because potential human bone marrow mesenchymal cells (BM MSCs) remains controversial, we have explored theory lineage-priming applied these cells. We show that primary layers and clones BM MSCs precise priming osteoblastic (O),...
Abstract Background Rheumatoid arthritis (RA) is a chronic, inflammatory and systemic autoimmune disease that leads to progressive cartilage destruction. Advances in the treatment of RA-related destruction require profound insights into molecular mechanisms involved degradation. Until now, comprehensive data about dysfunction chondrocytes have been limited. Hence, objective this study was establish standardized vitro model profile key regulatory molecules are expressed by human chondrocytes....
The basic helix-loop-helix transcriptional repressor twist1, as an antagonist of nuclear factor κB (NF-κB)–dependent cytokine expression, is involved in the regulation inflammation-induced immunopathology. We show that twist1 expressed by activated T helper (Th) 1 effector memory (EM) cells. Induction Th cells depended on NF-κB, (NFAT), and interleukin (IL)-12 signaling via signal transducer activator transcription (STAT) 4. Expression was transient after cell receptor engagement, increased...
Objective Rheumatoid arthritis (RA) accompanies infiltration and activation of monocytes in inflamed joints. We investigated dominant alterations RA bone marrow (BM), blood Methods CD14 + cells from BM peripheral (PB) patients with osteoarthritis (OA) were profiled GeneChip microarrays. Detailed functional analysis was performed reference transcriptomes precursors, monocyte subsets, mobilisation. Cytometric profiling determined subsets ++ CD16 − , BM, PB synovial fluid (SF) ELISAs quantified...
Gene expression profiling of peripheral blood mononuclear cells (PBMCs) has revealed a crucial role for type I interferon (IFN) in the pathogenesis systemic lupus erythematosus (SLE). However, it is unclear how particular leucocyte subsets contribute to overall IFN signature PBMCs and whole samples.Furthermore, detailed analysis describing differences autoimmune diseases from that observed after viral infection not been performed date. Therefore, this study, transcriptional responses T...
teaching and research institutions in France or abroad, from public private centers.L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement recherche français étrangers, laboratoires publics privés.
Fasting is beneficial in many diseases, including rheumatoid arthritis (RA), with lasting effects for up to 1 year. However, existing data dates back several decades before the introduction of modern therapeutic modalities.This exploratory RCT compares a 7-day fast followed by plant-based diet (PBD) dietary recommendations German society nutrition (Deutsche Gesellschaft für Ernährung, DGE) on RA disease activity, cardiovascular (CV) risk factors, and well-being.In this we randomly assigned...
The uptake mechanism for the spirochete Borrelia burgdorferi, causative agent of Lyme disease, was investigated by electron microscopy human and murine phagocytes. Spirochetes both a low- high-passage strain were preferentially internalized coiling rather than conventional phagocytosis. spirochetes engulfed phagocytosis found to disintegrate in an organelle exclusion zone without evident participation lysosomes. Preincubation B. burgdorferi with monoclonal antibody spirochetal OspA enhanced...
We performed transcription profiling using monocytes to identify predictive markers of response anti–tumor necrosis factor (anti-TNF) therapy in patients with rheumatoid arthritis (RA). Several potential predictors were identified, including CD11c. Validation samples from independent cohorts (total n = 27 patients) reverse transcription–PCR confirmed increased expression CD11c responders adalimumab (100% sensitivity; 91.7% specificity, power 99.6%; α 0.01). Pretherapy levels significantly...
Abstract Background The potential of mesenchymal stromal cells (MSCs) to differentiate into functional bone forming provides an important tool for regeneration. identification factors that trigger osteoblast differentiation in MSCs is therefore critical promote the osteogenic human MSCs. In this study, we used microarray analysis identify signalling molecules marrow stroma derived Results Microarray and validation experiments showed expression IGF2 IGFBP2 was increased together with integrin...
Repeatedly activated T helper 1 (Th1) cells present during chronic inflammation can efficiently adapt to the inflammatory milieu, for example, by expressing transcription factor Twist1, which limits immunopathology caused Th1 cells. Here, we show that in repeatedly murine cells, Twist1 and T‐bet induce expression of microRNA‐148a (miR‐148a). miR‐148a regulates proapoptotic gene Bim , resulting a decreased Bim/Bcl2 ratio. Inhibition antagomirs increases Bim, leading enhanced apoptosis....