A5045385495- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Psoriasis: Treatment and Pathogenesis
- IL-33, ST2, and ILC Pathways
- Cutaneous lymphoproliferative disorders research
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Inflammasome and immune disorders
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Mathematical Biology Tumor Growth
- Biosimilars and Bioanalytical Methods
- Cytomegalovirus and herpesvirus research
Kaiser Permanente South San Francisco Medical Center
2022
Genentech
2012-2015
German Rheumatism Research Centre
2004-2014
Leibniz Association
2011-2014
Charité - Universitätsmedizin Berlin
1997-2008
Zero to Three
2004
Humboldt-Universität zu Berlin
1997-2001
Humboldt State University
1998
Abstract Constitutive expression of CD25, the IL‐2 receptor α‐chain, defines a distinct population CD4 + T cells (Treg) with suppressive activity in vitro and vivo . has been implicated generation maintenance Treg, however, functional contribution during suppression is thus far unknown. We show that required for Treg function vitro, since completely abrogated by selective blocking on co‐culture responder cells. demonstrate which do not produce IL‐2, compete secreted In accordance idea...
Interferon regulatory factor 4 (IRF4) and IRF8 regulate B, T, macrophage, dendritic cell differentiation. They are recruited to cis-regulatory Ets-IRF composite elements by PU.1 or Spi-B. How these IRFs target genes in most T cells is enigmatic given the absence of specific Ets partners. Chromatin immunoprecipitation sequencing helper 17 (T(H)17) reveals that IRF4 targets sequences enriched for activating protein 1 (AP-1)-IRF (AICEs) co-bound BATF, an AP-1 required T(H)17, BATF bind...
CRISPR (clustered, regularly interspaced, short palindromic repeats)/Cas9 (CRISPR-associated protein 9) has become the tool of choice for generating gene knockouts across a variety species. The ability efficient editing in primary T cells not only represents valuable research to study function but also holds great promise cell–based immunotherapies, such as next-generation chimeric antigen receptor (CAR) cells. Previous attempts apply CRIPSR/Cas9 have resulted highly variable knockout...
Secretion of the immunosuppressive cytokine interleukin (IL) 10 by effector T cells is an essential mechanism self-limitation during infection. However, transcriptional regulation IL-10 expression in proinflammatory helper (Th) 1 insufficiently understood. We report a crucial role for regulator Blimp-1, induced IL-12 STAT4-dependent manner, controlling Th1 cells. Blimp-1 deficiency led to excessive inflammation Toxoplasma gondii infection with increased mortality. production from was...
T helper 1 (Th1) cells mediate powerful cellular immune responses. However, if unbalanced, Th1 immunity eventually may cause pathology. Recently, it has been shown that IL-10, an antiinflammatory cytokine strongly antagonizing Th1-mediated effects, can be produced by and is indeed essential for self-regulation of immunity. Here, we show Notch induces IL-10 production in newly developing already established via a signal transducer activator transcription 4 (STAT4)-dependent process. signaling...
Genome engineering of T lymphocytes, the main effectors antitumor adaptive immune responses, has potential to uncover unique insights into their functions and enable development next-generation adoptive cell therapies. Viral gene delivery cells, which is currently used generate CAR limitations in regard targeting precision, cargo flexibility, reagent production. Nonviral methods for effective CRISPR/Cas9-mediated knock-out primary human cells have been developed, but complementary techniques...
The basic helix-loop-helix transcriptional repressor twist1, as an antagonist of nuclear factor κB (NF-κB)–dependent cytokine expression, is involved in the regulation inflammation-induced immunopathology. We show that twist1 expressed by activated T helper (Th) 1 effector memory (EM) cells. Induction Th cells depended on NF-κB, (NFAT), and interleukin (IL)-12 signaling via signal transducer activator transcription (STAT) 4. Expression was transient after cell receptor engagement, increased...
The C-C motif chemokine receptor 8 (CCR8) is a class A G-protein coupled that has emerged as promising therapeutic target in cancer. Targeting CCR8 with an antibody appeared to be attractive approach, but the molecular basis for chemokine-mediated activation and antibody-mediated inhibition of are not fully elucidated. Here, we obtain antagonist against human determine structures complex either or endogenous agonist ligand CCL1. Our studies reveal characteristic features allowing recognition...
The Notch pathway is involved in cell differentiation processes various organs and at several developmental stages. importance of for early T lymphocyte development well established. Recently, has been implicated directing naive helper towards the Th1, Th2 or regulatory lineages. However, molecular events underlying these are poorly understood. We show that ligands Delta-like1, Delta-like4 Jagged1 differentially affect activation proliferation following receptor cross-linking. Delta-like1...