A5076232585- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune cells in cancer
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Forest ecology and management
- Phagocytosis and Immune Regulation
- Monoclonal and Polyclonal Antibodies Research
- Agricultural and Food Sciences
- Single-cell and spatial transcriptomics
- Ferroptosis and cancer prognosis
- Soil Management and Crop Yield
- Tryptophan and brain disorders
- Cancer Research and Treatments
- South Asian Studies and Conflicts
- Bladder and Urothelial Cancer Treatments
- Remote Sensing and LiDAR Applications
- FOXO transcription factor regulation
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Molecular Biology Techniques and Applications
- Mathematical Biology Tumor Growth
- Peace and Human Rights Education
Theravance Biopharma (United States)
2021-2023
Sunesis (United States)
2020
Vaccine & Gene Therapy Institute of Florida
2012-2017
Universidade Federal de Santa Maria
2014-2016
Universidade do Contestado
2016
Weatherford College
2016
Universidade do Estado de Santa Catarina
2016
Vaccine and Gene Therapy Institute
2012-2014
Baylor College of Medicine
2007-2013
Université de Montréal
2011
In the present study, we have investigated distribution of HIV-specific and HIV-infected CD4 T cells within different populations memory isolated from lymph nodes viremic subjects. Four cell were identified on basis expression CXCR5, PD-1, Bcl-6: CXCR5−PD-1−Bcl-6−, CXCR5+PD-1−Bcl-6−, CXCR5−PD-1+Bcl-6−, CXCR5+PD-1+Bcl-6+. On Bcl-6 functional properties (IL-21 production B help), CXCR5+PD-1+Bcl-6+ population was considered to correspond follicular helper (Tfh) population. We show that Tfh...
Dual blockade of the PD-1 and TIGIT coinhibitory receptors on T cells shows promising early results in cancer patients. Here, we studied mechanisms whereby and/or modulate anti-tumor CD8+ cells. Although are thought to regulate different costimulatory (CD28 CD226), effectiveness or inhibition preclinical tumor models was reduced absence CD226. CD226 expression associated with clinical benefit patients non-small cell lung carcinoma (NSCLC) treated anti-PD-L1 antibody atezolizumab. CD28 were...
Background. Defining the parameters that modulate vaccine responses in African populations will be imperative to design effective vaccines for protection against HIV, malaria, tuberculosis, and dengue virus infections. This study aimed evaluate contribution of patient-specific immune microenvironment response licensed yellow fever 17D (YF-17D) an cohort.
Checkpoint inhibitors like anti-PD1/PD-L1 have demonstrated significant therapeutic efficacy in a subset of patients partly through reinvigoration CD8 T cells. However, their impact on myeloid cells remains largely unknown. Here, we report that anti-PD-L1 treatment favorably impacts the phenotype and function tumor macrophages by polarizing macrophage compartment toward more proinflammatory phenotype. This was characterized decrease Arginase-I (ARG1) expression an increase iNOS, MHCII, CD40...
Abstract Background Trop2 is a cell-surface glycoprotein overexpressed by variety of epithelial carcinomas with reported low to restricted expression in normal tissues. Expression has been associated increased tumor aggressiveness, metastasis and decreased patient survival, but the signaling mechanisms mediated are still unknown. Here, we studied effects murine (mTrop2) exerted on cellular functions some activated this oncogene. Results mTrop2 significantly cell proliferation at serum...
Aging leads to dysregulation of multiple components the immune system that results in increased susceptibility infections and poor response vaccines aging population. The dysfunctions adaptive B T cells are well documented, but effect on innate immunity remains incompletely understood. Using a heterogeneous population peripheral blood mononuclear (PBMCs), we first undertook transcriptional profiling found PBMCs isolated from old individuals (≥ 65 years) exhibited delayed altered stimulation...
Virus-like particles (VLPs) have gained increasing interest for their use as vaccines due to repetitive antigenic structure that is capable of efficiently activating the immune system. The efficacy VLP immunization may lie in its ability traffic into draining lymph nodes while antigen-presenting cells initiate orchestration signals required development a robust response. Currently, there no comprehensive study showing correlation different vaccination routes outcome. In this study, we took...
CD96 is a member of the poliovirus receptor (PVR, CD155)-nectin family that includes T cell Ig and ITIM domain (TIGIT) CD226. While CD96, TIGIT, CD226 have important roles in regulating NK activity, TIGIT also been shown to regulate responses, it unclear whether has inhibitory or stimulatory function CD8+ cells. Here, we demonstrate co-stimulatory on Crosslinking human mouse cells induced activation, effector cytokine production, proliferation. was found transduce its activating signal...
TGFβ signaling is associated with non-response to immune checkpoint blockade in patients advanced cancers, particularly the immune-excluded phenotype. While previous work demonstrates that converting tumors from excluded inflamed phenotypes requires attenuation of PD-L1 and signaling, underlying cellular mechanisms remain unclear. Here, we show restrain intratumoral stem cell-like CD8 T cell (TSCL) expansion replacement progenitor-exhausted dysfunctional cells non-exhausted effector EMT6...
Loss of memory B cells occurs from the onset HIV-1 infection and persists into chronic stages infection. Lack survival these cells, even in subjects being treated, could primarily be consequence an altered local microenvironment induced by HIV In this study we showed that cell was significantly decreased aviremic successfully treated (ST) compared with who control viral load as a result natural immunity (elite controller [EC]) or uninfected (HIV-) subjects. The lower levels observed ST were...
Despite the overwhelming benefits of antiretroviral therapy (ART) in curtailing viral load HIV-infected individuals, ART does not fully restore cellular and humoral immunity. individuals under show reduced responses to vaccination infections are unable mount an effective antiviral immune response upon cessation. Many factors contribute these defects, including persistent inflammation, especially lymphoid tissues, where T follicular helper (Tfh) cells instruct help B launch response. In this...
HIV-1-specific broadly neutralizing antibodies (bnAbs) typically develop in individuals with continuous high-level viral replication and increased immune activation, conditions that cannot be reproduced during prophylactic immunization. Understanding mechanisms supporting bnAb development the absence of viremia may important for designing bnAb-inducing immunogens. Here, we show breadth antibody responses HIV-1 controllers was associated a relative enrichment circulating CXCR5+CXCR3+PD-1lo...
Natural killer (NK) cell recognition of tumor cells is mediated through activating receptors such as CD226, with suppression effector functions often controlled by negative regulatory transcription factors FOXO1. Here we show that CD226 regulation NK cytotoxicity facilitated inactivation Gene-expression analysis isolated from syngeneic tumors grown in wild-type or CD226-deficient mice revealed dysregulated expression FOXO1-regulated genes the absence CD226. In vitro and stimulation assays...
Abstract Given the critical role of mucosal surfaces in susceptibility to infection, it is imperative that effective responses are induced when developing efficacious vaccines and prevention strategies for infection. Modulating microbiota gastrointestinal (GI) tract through use probiotics (PBio) a safe well-tolerated approach enhance overall health. We assessed longitudinal impact daily treatment with VSL#3 probiotic on cellular humoral immunity inflammation healthy macaques. PBio therapy...
Exhausted T cells have been described in cancer patients and murine tumor models largely based on their expression of various inhibitory receptors. Understanding the functional attributes these is limited. Here, we report that among CD8+ commonly used syngeneic models, coexpression receptors PD-1, LAG3, TIM3 defined a group highly activated effector cells. Coexpression further enriched for antigen-specific with increased T-cell receptor clonality. Anti-PD-L1 treatment number activation...
Trop2 is a recently discovered cell surface glycoprotein overexpressed in pancreatic cancer which could potentially be used as an immunotherapeutic target. Enveloped virus-like particles (VLPs) are highly immunogenic and versatile immune stimulatory agents can modified to incorporate exogenous proteins on their membrane envelope use vaccines. In this study, we investigated the effects of murine (mTrop2) VLP immunization syngeneic model. VLPs incorporating mTrop2 were immunize C57BL/6...
Abstract Immunotherapy with checkpoint inhibitors has proved to be highly effective, durable responses in a subset of patients. Given their encouraging clinical activity, are increasingly being tested trials combination chemotherapy. In many instances, there is little understanding how chemotherapy might influence the quality immune response generated by inhibitors. this study, we evaluated impact alone or anti–PD-L1 responsive syngeneic tumor model. Although multiple classes treatment...
Agonism of members the tumor necrosis factor receptor superfamily (TNFRSF) with monoclonal antibodies is high therapeutic interest due to their role in immune regulation and cell proliferation. A major hurdle for pharmacologic activation this class requirement high-order clustering, a mechanism that imposes reliance vivo on Fc receptor-mediated crosslinking. This extrinsic dependence represents potential limitation virtually entire pipeline agonist TNFRSF antibody drugs, which none have thus...
Background Cancer immunotherapy has evolved from interferon-alpha (IFNα) and interleukin-2 in the 1980s to CTLA-4 PD-1/PD-L1 checkpoint inhibitors (CPIs), latter highlighting importance of enhancing T-cell functions. While search for novel immunomodulatory pathways continues, combination therapies augmenting multiple can also increase efficacy. The association autoimmune-related adverse events with clinical efficacy following CPI treatment been inferred suggests that breaking tolerance...