A5004383207- Immune Cell Function and Interaction
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Diversity and Career in Medicine
- IL-33, ST2, and ILC Pathways
- Medical Education and Admissions
- Cytomegalovirus and herpesvirus research
- HIV/AIDS Research and Interventions
- Hepatitis Viruses Studies and Epidemiology
- HIV/AIDS drug development and treatment
- Healthcare professionals’ stress and burnout
- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Respiratory viral infections research
- Primary Care and Health Outcomes
- Healthcare cost, quality, practices
- Patient Safety and Medication Errors
- Fetal and Pediatric Neurological Disorders
Drexel University
2016-2025
University of Massachusetts Chan Medical School
2024
The University of Texas Health Science Center at Houston
2024
University Health Network
2021
Toronto Western Hospital
2021
The Wilson Centre
2021
Arizona State University
2021
University of Nebraska Medical Center
2021
Mayo Clinic in Florida
2021
University of Chicago
2021
Rationale: IL-17A is purported to help drive early pathogenesis in acute respiratory distress syndrome (ARDS) by enhancing neutrophil recruitment. Although the archetypal cytokine of T-helper 17 cells, it produced a number lymphocytes, source during ARDS being unknown.Objectives: To identify cellular and role phase lung injury.Methods: Lung injury was induced wild-type (C57BL/6) IL-17 knockout (KO) mice with aerosolized LPS (100 μg) or Pseudomonas aeruginosa infection. Detailed phenotyping...
CD8+ T cells play a critical role in controlling HIV viremia and could be important reducing HIV-infected approaches to eradicate HIV. The simian immunodeficiency virus model provided the proof of concept for cell-mediated reservoir clearance but showed conflicting evidence on these eliminate cells. In humans, HIV-specific cell responses have not been associated with reduction pool vivo. We studied RV254 cohort individuals initiating ART earliest stages acute infection (AHI). that generated...
Despite the overwhelming benefits of antiretroviral therapy (ART) in curtailing viral load HIV-infected individuals, ART does not fully restore cellular and humoral immunity. individuals under show reduced responses to vaccination infections are unable mount an effective antiviral immune response upon cessation. Many factors contribute these defects, including persistent inflammation, especially lymphoid tissues, where T follicular helper (Tfh) cells instruct help B launch response. In this...
Schistosomiasis is a disease caused by parasitic flatworms of the Schistosoma spp ., and increasingly recognized to alter immune system, potential respond vaccines. The impact endemic infections on protective immunity critical inform vaccination strategies globally. We assessed influence mansoni worm burden multiple host vaccine-related parameters in Ugandan fishing cohort (n = 75) given three doses Hepatitis B (HepB) vaccine at baseline timepoints post-vaccination. observed distinct...
Acute respiratory distress syndrome (ARDS) is a devastating disorder characterized by increased alveolar permeability with no effective treatment beyond supportive care. Current mechanisms underlying ARDS focus on endothelial and epithelial injury caused products of innate immune cells platelets. However, the role adaptive in remains largely unknown. In this study, we report that expansion Ag-specific αβTh17 contributes to local secretion IL-17A, which turn directly increases permeability....
Plasmacytoid dendritic cells (pDCs) are robust producers of IFNα and one the first immune to respond SIV infection. To elucidate responses early HIV-1 replication, we studied blood pDCs in 29 HIV-infected participants who initiated antiretroviral therapy during acute infection underwent analytic treatment interruption (ATI). We observed an increased frequency partially activated before detection HIV RNA. Concurrent with peak pDC frequency, detected a transient decline ability produce vitro,...
ABSTRACT Lymph node T follicular helper (Tfh) cells and germinal center (GC) B are critical to generate potent antibodies but rarely possible study in humans. To understand how Tfh/GC B-cell interactions during acute HIV-1 infection (AHI) impact the generation of HIV-specific antibodies, we performed a unique cross-sectional analysis inguinal lymph biopsies taken prior antiretroviral therapy (ART) initiation AHI. Although total Tfh GC cell frequencies did not change AHI, increased...
Abstract IL-9 is a cytokine of great current interest associated with allergic/Th2 responses. High levels are present in bronchial secretions from infants respiratory syncytial virus (RSV) bronchiolitis. To test its effects RSV disease Th2 profile, BALB/c mice were vaccinated recombinant vaccinia expressing the G protein. On challenge, immunized developed augmented characterized by enhanced pulmonary and local production peaking on days 7–10 infection. Depletion anti-IL-9 Ab at vaccination...
Abstract Follicular helper T cells (Tfh) play critical roles instructing, and initiating T-cell dependent antibody responses. The underlying mechanisms that enhance their function is therefore for vaccine development. Here we apply gene array analysis identifying adenosine deaminase (ADA) as a key molecule delineates human Tfh program in proliferating circulating (cTfh) Germinal Centers (GC-Tfh). ADA-1 expression enzymatic activity are increased efficient cTfh2-17/GC-Tfh cells. Exogenous...
Abstract Innate Lymphoid Cells (ILCs) are immune cells typically found on mucosal surfaces and in secondary lymphoid organs where they regulate the response to pathogens. Despite their key role response, there still fundamental gaps our understanding of ILCs. Here we report a human ILC population present follicles tonsils lymph nodes termed follicular regulatory ILCs (ILC FR ) that knowledge has not been previously identified. have distinct phenotype transcriptional program when compared...
The RV254 cohort of HIV-infected very early acute (4thG stage 1 and 2) (stage 1/2) late 3) individuals was used to study T helper- B cell responses in HIV infection the impact antiretroviral treatment (ART) on function. To investigate this, function circulating follicular helper cells (cTfh) from this examined, cTfh memory populations were phenotyped. Impaired observed treated 3 when compared 1/2. cTfh/B cocultures showed lower survival IgG secretion at This coincided with IL-10 increased...
Early initiation of antiretroviral therapy (ART) in acute HIV infection (AHI) is effective at limiting seeding the viral reservoir, but little known about how resultant decreased antigen load affects long-term Ab development after ART. We report here that Env-specific plasma antibody (Ab) levels and Ab-dependent cellular cytotoxicity (ADCC) increased during first 24 weeks ART correlated with persisting 48 Participants treated AHI stage 1 had lower ADCC activity on than did those later....
An effective vaccine to prevent HIV transmission has not yet been achieved. Modulation of the microbiome via probiotic therapy suggested result in enhanced mucosal immunity. Here, we evaluated whether could improve immunogenicity and protective efficacy SIV/HIV vaccination. Rhesus macaques were co-immunized with an DNA particle-mediated epidermal delivery protein administered intramuscularly Adjuplex™ adjuvant, while receiving daily oral Visbiome® probiotics. Probiotic alone led reduced...
Streptococcus pyogenes is the causative agent of numerous diseases ranging from benign infections (pharyngitis and impetigo) to severe associated with high mortality (necrotizing fasciitis bacterial sepsis). As other infections, there considerable interest in characterizing contribution interleukin-17A (IL-17A) responses protective immunity. We here show significant il17a up-regulation by quantitative real-time PCR secondary lymphoid organs, correlating increased protein levels serum within...
A role for interleukin-21 (IL-21) has recently been found in several diseases, but contribution to mucosal defences not described. In BALB/c mice infected with respiratory syncytial virus (RSV), IL-21 depletion had little effect primary infection. However, of during priming recombinant vaccinia expressing RSV G protein (which primes RSV-specific T helper type 2 cells and causes lung eosinophilia infection) further exacerbated pathology challenge, reduced viral clearance impaired...
There is currently no prophylactic vaccine available for human immunodeficiency virus (HIV). Research efforts have resulted in improved immunogens that mimic the native envelope (Env) glycoprotein structure. Recently, a novel triple tandem trimer (TTT) platform has been used to generate plasmid encoding Env immunogen (pBG505-TTT) expresses only as trimers, making it more suitable nucleic acid vaccines. We previously demonstrated adenosine deaminase-1 (ADA-1) critical T follicular helper...