A5015543845- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Hippo pathway signaling and YAP/TAZ
- Chronic Lymphocytic Leukemia Research
- HER2/EGFR in Cancer Research
- Melanoma and MAPK Pathways
- Computational Drug Discovery Methods
- Phagocytosis and Immune Regulation
- Pancreatitis Pathology and Treatment
- Galectins and Cancer Biology
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- Cancer Immunotherapy and Biomarkers
- Protein Kinase Regulation and GTPase Signaling
- Asthma and respiratory diseases
- Toxoplasma gondii Research Studies
- Wnt/β-catenin signaling in development and cancer
- Peptidase Inhibition and Analysis
- Reproductive tract infections research
- Immunodeficiency and Autoimmune Disorders
- Ubiquitin and proteasome pathways
- Microbial infections and disease research
Institute of Automation
2010
Genentech
2010
EA Technology
2007
Rockefeller University
1972
Tim-4 is a phosphatidylserine (PS) receptor that expressed on various macrophage subsets. It mediates phagocytosis of apoptotic cells by peritoneal macrophages. The in vivo functions and immune responses, however, are still unclear. In this study, we show quickly forms punctate caps contact with cells, contrast to its normal diffused expression the surface phagocytes. Despite marginal zone tingible body macrophages, deficiency only minimally affects outcomes several acute challenges,...
The LAP (leucine-rich repeatand PDZ-containing) family of proteins play a role in maintaining epithelial and neuronal cell size, mutation these can have oncogenic consequences. protein Erbin has been implicated previously number cellular activities by virtue its PDZ domain-dependent association with the C termini both ERB-B2 p120-catenins. present work describes NMR structure complex high affinity peptide ligand includes comprehensive energetic analysis domain side chains responsible for...
IgE-mediated hypersensitivity is central to the pathogenesis of asthma and other allergic diseases. Although neutralization serum IgE with IgE-specific antibodies in general an efficacious treatment for asthma, one limitation this approach its lack effect on production. Here, we have developed a strategy disrupt production by generating monoclonal that target segment membrane human IgE-switched B cells not present IgE. This known as M1' domain, using genetically modified mice contain domain...
We present a detailed comparative analysis of the PDZ domains human LAP proteins Erbin, Densin-180, and Scribble MAGUK ZO-1. Phage-displayed peptide libraries in vitro affinity assays were used to define ligand binding profiles for each domain. The reveals importance interactions with all four C-terminal residues ligand, which constitute core recognition motif, also role more upstream that support modulate interaction. In particular, results highlight site−1, interacts penultimate residue C...
Studies have been made on factors which influence attachment of Mycoplasma pulmonis to the surface mouse macrophages in vitro, and determine whether or not attached microorganisms are ingested.
Agonism of members the tumor necrosis factor receptor superfamily (TNFRSF) with monoclonal antibodies is high therapeutic interest due to their role in immune regulation and cell proliferation. A major hurdle for pharmacologic activation this class requirement high-order clustering, a mechanism that imposes reliance vivo on Fc receptor-mediated crosslinking. This extrinsic dependence represents potential limitation virtually entire pipeline agonist TNFRSF antibody drugs, which none have thus...
In developing a screening assay for serine/threonine kinase, we evaluated various formats of an in-plate enzyme-linked immunosorbent (ELISA), as well solution-phase kinase assays using either ELISA or AlphaScreen detection. Substrate was available both biotinylated 15-residue peptide and 25-residue containing the same sequence expressed glutathione S-transferase fusion protein. When increasing concentrations these substrates were coated directly onto plates, rates reactions progressively...
Abstract Pathogenic B lymphocytes removal through therapy with depleting monoclonal antibodies results in clinical benefit both oncology and immunological indications. Incomplete rates of response the realization that cell depletion following immunotherapy is incomplete fuels need for better therapies. BAFF/BLyS signaling its BAFFR/BR3 receptor have a critical role on proliferation survival mouse to some extent non-human primates humans. In order combine blockade BAFF mediated survival, we...
Abstract Agonistic antibodies to co-stimulatory members of the TNF Receptor Superfamily (TNFRSF) have demonstrated impressive antitumor immune responses in murine tumor models and are currently under investigation clinic. In order induce efficient signaling, majority TNFRSF require supermolecular cluster formation on cell surface. Recent studies revealed that agonistic achieve this part by Fcγ receptor (FcγR) mediated cross-linking, enabling member expressed opposing cell. OX40 (CD134;...