A5068305875- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Histone Deacetylase Inhibitors Research
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Lymphoma Diagnosis and Treatment
- Cancer-related gene regulation
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- interferon and immune responses
- Botanical Research and Applications
- Monoclonal and Polyclonal Antibodies Research
- Sunflower and Safflower Cultivation
- Botany, Ecology, and Taxonomy Studies
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
Children's Hospital of Los Angeles
2015-2022
La Roche College
2021
Mashhad University of Medical Sciences
2017
The Ohio State University
2012-2016
Institut thématique Génétique, génomique et bioinformatique
2012
Ohio University
2012
University of Cincinnati
2008-2011
Genome engineering of T lymphocytes, the main effectors antitumor adaptive immune responses, has potential to uncover unique insights into their functions and enable development next-generation adoptive cell therapies. Viral gene delivery cells, which is currently used generate CAR limitations in regard targeting precision, cargo flexibility, reagent production. Nonviral methods for effective CRISPR/Cas9-mediated knock-out primary human cells have been developed, but complementary techniques...
The molecular interactions between B-cell precursor acute lymphoblastic leukemia (pre-B ALL) cells and stromal in the bone marrow that provide microenvironmentally-mediated protection against therapeutic drugs are not well-defined. Galectin-3 (Lgals3) is a multifunctional galactose-binding lectin with reported location nucleus, cytoplasm extracellular space different cell types. We previously ALL co-cultured stroma contain high levels of Galectin-3. here establish that, contrast to more...
Aberrant expression of the secreted protein, acidic, cysteine-rich (osteonectin) (SPARC) gene, which encodes a matricellular protein that participates in normal tissue remodeling, is associated with variety diseases including cancer, but contribution SPARC to malignant growth remains controversial. We previously reported was among most upregulated genes cytogenetically acute myeloid leukemia (CN-AML) patients gene-expression profiles predictive unfavorable outcome, such as mutations...
Deregulation of microRNAs' expression frequently occurs in acute myeloid leukemia (AML). Lower miR-181a is associated with worse outcomes, but the exact mechanisms by which mediates this effect remain elusive. Aberrant activation RAS pathway contributes to leukemogenesis. Here, we report that directly binds 3'-untranslated regions (UTRs); downregulates KRAS, NRAS and MAPK1; decreases AML growth. The delivery mimics target cells using transferrin-targeting lipopolyplex nanoparticles (NP)...
Mexico has long been recognized as one of the world's cradles domestication with evidence for squash ( Cucurbita pepo ) cultivation appearing early 8,000 cal B.C. followed by many other plants, such maize Zea mays ), peppers Capsicum annuum common beans Phaseolus vulgaris and cotton Gossypium hirsutum ). We present archaeological, linguistic, ethnographic, ethnohistoric data demonstrating that sunflower Helianthus annuus had entered repertoire Mexican domesticates ca. 2600 B.C., its was...
Hepatocellular carcinoma (HCC) develops in the context of chronic inflammatory liver disease and has an extremely poor prognosis. An immunosuppressive tumor microenvironment may contribute to therapeutic failure metastatic HCC. Here, we identified unique molecular signatures pertaining HCC progression immunity by analyzing genome-wide RNA-Seq data derived from patient tumors non-tumor cirrhotic tissues. Unsupervised clustering gene expression revealed a gradual suppression local that...
Regulatory T cells (Treg) are immunosuppressive and negatively impact response to cancer immunotherapies. CREB-binding protein (CBP) p300 closely related acetyltransferases transcriptional coactivators. Here, we evaluate the mechanisms by which CBP/p300 regulate Treg differentiation consequences of loss-of-function mutations in follicular lymphoma. Transcriptional epigenetic profiling identified a cascade transcription factors essential for differentiation. Mass spectrometry analysis showed...
Galectin-3 is a β-galactoside-specific, carbohydrate-recognizing protein (lectin) that strongly implicated in cancer development, metastasis, and drug resistance. promotes migration ability to withstand treatment of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) cells. Due high amino acid conservation among galectins the shallow nature their glycan-binding site, design selective potent antagonists targeting galectin-3 challenging. Herein, we report synthesis novel taloside-based...
Environmentally-mediated drug resistance in B-cell precursor acute lymphoblastic leukemia (BCP-ALL) significantly contributes to relapse. Stromal cells the bone marrow environment protect by secretion of chemokines as cues for BCP-ALL migration towards, and adhesion to, stroma. communicate through stromal galectin-3. Here, we investigated significance galectin-3 cells. We used CRISPR/Cas9 genome editing ablate found that is dispensable steady-state proliferation viability. However, efficient...
Genetically engineered CD8 + T cells are being explored for the treatment of various cancers. Analytical characterization represents a major challenge in development genetically cell therapies, especially assessing potential off-target editing and product heterogeneity. As conventional sequencing techniques only provide information at bulk level, they unable to detect CRISPR translocation or events occurring minor subpopulations. In this study, we report analytical single-cell multi-omics...
Acute lymphoblastic leukemias arising from the malignant transformation of B-cell precursors (BCP-ALLs) are protected against chemotherapy by both intrinsic factors as well interactions with bone marrow stromal cells. Galectin-1 and Galectin-3 lectins overlapping specificity for binding polyLacNAc glycans. Both expressed cells hematopoietic but show different patterns expression, dynamically regulated extrinsic such chemotherapy. In a comparison x double null mutant to wild-type murine...
Triplex gene editing relies on binding a stable peptide nucleic acid (PNA) sequence to chromosomal target, which alters the helical structure of DNA stimulate site-specific recombination with single-strand (ssDNA) donor template and elicits correction. Here, we assessed whether codelivery PNA encapsulated in Poly Lactic-co-Glycolic Acid (PLGA)-based nanoparticles can correct sickle cell disease x-linked severe combined immunodeficiency. However, through this process have identified...
Abstract With standard cytotoxic chemotherapy the majority of patients with Acute Myeloid Leukemia (AML) fail to achieve long term remission. Protein Arginine Methyltransferase 5 (PRMT5) can catalyze methylation arginine residues in target proteins like histones and P53. The epigenetic marks PRMT5 on H3 (S2Me-H3R8) H4 (S2Me-H4R3), directly lead transcriptional silencing genes. Overexpression has been reported hematologic solid malignancies represents a promising therapeutic target. Thus, we...
<p>Supplementary figure 6 shows additional assessments of B cells in FL patient tissue samples and their activation status supportive vitro findings.</p>
<p>Supplementary figure 2 shows supportive naïve T cell transcriptional profiling data and additional TF analyses.</p>