A5060787894- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Mechanisms of cancer metastasis
- Kruppel-like factors research
- Prostate Cancer Treatment and Research
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Sulfur Compounds in Biology
- Hormonal and reproductive studies
- Folate and B Vitamins Research
- DNA Repair Mechanisms
- Amino Acid Enzymes and Metabolism
- Uterine Myomas and Treatments
- TGF-β signaling in diseases
- Microtubule and mitosis dynamics
- Receptor Mechanisms and Signaling
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Sirtuins and Resveratrol in Medicine
- Galectins and Cancer Biology
- Nanoparticle-Based Drug Delivery
University of Oklahoma Health Sciences Center
2015-2024
University of Oklahoma
2023
Indian Institute of Technology Kharagpur
2022
University of Kalyani
2019
Oklahoma State University Oklahoma City
2016
OU Health Stephenson Cancer Center
2016
Florida College
2013-2016
University of Florida
2013-2016
Institute of Post Graduate Medical Education and Research
2010-2012
Bmi-1 is a member of the Polycomb Repressor Complex1 that mediates gene silencing by regulating chromatin structure and indispensable for self-renewal both normal cancer stem cells. Despite three decades research have elucidated transcriptional regulation, post-translational modifications functions in DNA damage response, cellular bioenergetics, pathologies, entire potential protein with such varied function remains to be realized. This review attempts synthesize current knowledge on an...
We have previously demonstrated that resveratrol (Resv)-induced cellular apoptosis occurs after formation of reactive oxygen species (ROS) but the role GSH has not been well defined. Our experimental data enumerated Resv treatment (50 μm) induced in human leukemic monocyte lymphoma cells, which was preceded by efflux. High concentration extracellular thiol (GSH, <i>N</i>-acetyl cysteine) and two specific inhibitors carrier-mediated extrusion, methionine or cystathionine, prevented process...
The clonal self-renewal property conferred by BMI1 is instrumental in maintenance of not only normal stem cells but also cancer-initiating from several different malignancies that represent a major challenge to chemotherapy. Realizing the immense pathological significance, PTC-209, small molecule inhibitor transcription has recently been described. While targeting various systems significantly decreases growth, mechanisms differ, are context-dependent, and somewhat unclear. We report here...
Activated cancer-associated fibroblasts (CAFs) play a major role in the poor outcome many diseases including pancreatic cancer. Normally quiescent with high lipid content and low proliferative capacity, CAFs receiving cues from cancer cells tumor microenvironment become activated transformed into lipid-deprived highly myofibroblast type phenotype. Therefore, reversal of to quiescence state is an important area investigation that may help therapeutic management number Here, we describe unique...
It is currently recognized that perpetual cross talk among key players in tumor microenvironment such as cancer cells (CCs), associated fibroblasts (CAFs), and endothelial (ECs) plays a critical role progression, metastasis, therapy resistance. Disruption of the may be useful to improve outcome therapeutics for which limited options are available. In current study we investigate use gold nanoparticles (AuNPs) therapeutic tool disrupt multicellular within TME with an emphasis on inhibiting...
The stringent expression of the hypoxia inducible factor-1α (HIF-1α) is critical to a variety pathophysiological conditions. We reveal that, in normoxia, enzymatic action cystathionine β-synthase (CBS) produces H
Cancer-associated fibroblasts (CAFs) are a major constituent of the tumor microenvironment (TME) and an important contributor to cancer progression therapeutic resistance. Regulation CAF activation is promising strategy influence outcomes. Here, we report that ovarian cells (OCs) TME promote CAFs, whereas gold nanoparticles (GNPs) 20 nm in diameter inhibit activation, as demonstrated by changes cell morphology, migration, molecular markers. GNPs exert effect altering levels multiple...
Hepatocellular carcinoma (HCC) develops in the context of chronic inflammatory liver disease and has an extremely poor prognosis. An immunosuppressive tumor microenvironment may contribute to therapeutic failure metastatic HCC. Here, we identified unique molecular signatures pertaining HCC progression immunity by analyzing genome-wide RNA-Seq data derived from patient tumors non-tumor cirrhotic tissues. Unsupervised clustering gene expression revealed a gradual suppression local that...
BMI-1, also known as a stem cell factor, is frequently upregulated in several malignancies. Elevated expression of BMI-1 correlates with poor prognosis and therefore considered viable therapeutic target number malignancies including ovarian cancer. Realizing the immense pathologic significance small-molecule inhibitors against are recently being developed. In this study, we functionally characterize PTC-028, an orally bioavailable compound that decreases levels by posttranslational...
Deregulation of mitochondrial morphogenesis, a dynamic equilibrium between fusion and fission processes, is now evolving as key metabolic event that fuels tumor growth therapy resistance. However, fundamental knowledge underpinning how cancer cells reprogram morphogenesis remains incomplete. Here, we report cystathionine β-synthase (CBS) reprograms in ovarian (OvCa) by selectively regulating the stability mitofusin 2 (MFN2). Clinically, high expression both CBS MFN2 implicates poor overall...
Astrocytic dysfunction is central to age-related neurodegenerative diseases. However, the mechanisms leading astrocytic are not well understood. We identify that among diverse cellular constituents of brain, murine and human astrocytes enriched in expression CBS. Depleting CBS causes mitochondrial dysfunction, increases production reactive oxygen species (ROS) decreases bioenergetics can be partially rescued by exogenous H
Mutations in the human cystathionine beta synthase (CBS) gene are known to cause endothelial dysfunction responsible for cardiovascular and neurovascular diseases. CBS is predominant hydrogen sulfide (H2 S)-producing enzyme cells (ECs). Recently, H2 S was shown attenuate ROS improve mitochondrial function. Mitochondria metabolic organelles that actively transform their ultrastructure mediate Therefore, we questioned whether perturbation of CBS/H2 activity could drive via dynamics ECs. Here...
Abstract By exploiting the self‐therapeutic properties of gold nanoparticles (GNPs) a molecular axis that promotes growth high‐grade serous ovarian cancer (HGSOC), one deadliest gynecologic malignancies with poorly understood underlying mechanisms, has been identified. The biodistribution and toxicity GNPs administered by intravenous or intraperitoneal injection, both as single dose repeated dosing over two weeks are first assessed; no biochemical histological to vital organs is found. Using...
Antiandrogens target ligand-binding domain of androgen receptor (AR) and are used as first-line therapeutics to treat patients diagnosed with locally advanced metastatic prostate cancer. Although initially beneficial judged actual tumor mass shrinkage, this therapy invariably fails the cancer reappears castration-resistant disease. Here, we report that increased intracellular nitric oxide (NO) levels lead growth inhibition both androgen-dependent tumors through a mechanism involves AR...
Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol promising compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid such an ulcerogenic effect. Consequently, here we tried to explore efficacy HST-1 compared with in different cancer cell lines and identified probable signaling pathways responsible for death. Our viability study HST-1, resveratrol, showed better potential all...
With rising incidence rates, endometrial cancer is one of the most common gynecologic malignancies in United States. Although surgery provides significant survival benefit to early-stage patients, those with advanced or recurrent metastatic disease have a dismal prognosis. Limited treatment options include chemotherapy and radiotherapy. Hence, there compelling need for developing molecularly targeted therapy. Here, we show that polycomb ring finger protein BMI1, also known as stem cell...
The cystathionine ß-synthase (CBS) is a critical enzyme in the transsulfuration pathway and responsible for synthesis of from serine homocysteine. Cystathionine precursor to amino acid cysteine. CBS also generation hydrogen sulfide (H2S) Mutation causes homocysteine levels rise, gives rise condition called hyperhomocysteinuria. To date, numerous mouse knockout models has been generated, which show panoply defects, reflecting importance this development. In zebrafish, we others have...
Macropinocytosis supports the metabolic requirement of RAS-transformed pancreatic ductal adenocarcinoma cells (PDACs). However, regulators RAS-transformation (activation) that lead to macropinocytosis have not been identified. Herein, we report UBAP2 (ubiquitin-binding associated protein 2), regulates activation KRAS and in cancer. We demonstrate is highly expressed both cancer cell lines tumor tissues PDAC patients. The expression with poor overall survival several cancers, including PDAC....
Uterine carcinosarcoma (UCS) is a relatively infrequent, but extremely aggressive endometrial malignancy. Although surgery and chemotherapy have improved outcomes, overall survival (OS) remains dismal due to the lack of targeted therapy biphasic (epithelial mesenchymal) nature that renders tumor difficult manage. Here we report role transforming growth factor-β (TGFβ) in maintaining epithelial mesenchymal transition (EMT) phenotype aggressiveness UCS. Using 3D-culture system, evaluated...
The present investigation was aimed to study the antiproliferative action of tamoxifen citrate (TMX), a non steroidal antiestrogen, on human T-cell leukemia cell line, Jurkat, as free drug and TMX loaded guar gum nanoparticles. For this we developed new formulation containing chemically cross-linked nanoparticles (GG NPs) with (TMX). Single step (oil in water) emulsion in-situ polymer cross-linking technique employed prepare spherical smooth surfaced size range 200-300nm. Nanoparticle shape...