A5068423984- Rheumatoid Arthritis Research and Therapies
- Mesenchymal stem cell research
- Bone and Joint Diseases
- Spondyloarthritis Studies and Treatments
- Autoimmune and Inflammatory Disorders Research
- Immune Cell Function and Interaction
- Osteoarthritis Treatment and Mechanisms
- Systemic Lupus Erythematosus Research
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Systemic Sclerosis and Related Diseases
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Psoriasis: Treatment and Pathogenesis
- Tryptophan and brain disorders
- MicroRNA in disease regulation
- Peripheral Neuropathies and Disorders
- Extracellular vesicles in disease
- IL-33, ST2, and ILC Pathways
- Stress Responses and Cortisol
- Inflammatory mediators and NSAID effects
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
National Institute of Geriatrics, Rheumatology and Rehabilitation
2010-2024
Medical University of Warsaw
2023
Objectives The aim of our study was to determine whether there is a correlation between transcription factors expression and Th17/Treg ratio, cytokine profile in the RA phenotype as well identify that could be potential biomarker for RA. Methods conducted on 45 patients with RA, 27 OA 46 healthy controls (HCs). Th17 Treg frequency determined by flow cytometry (15 RA/OA 15 subjects HC). Gene estimated qPCR, serum levels were ELISA. Results percentage (CD4+CD25highCD127-) cells lower than or...
Objective Rheumatoid arthritis (RA) accompanies infiltration and activation of monocytes in inflamed joints. We investigated dominant alterations RA bone marrow (BM), blood Methods CD14 + cells from BM peripheral (PB) patients with osteoarthritis (OA) were profiled GeneChip microarrays. Detailed functional analysis was performed reference transcriptomes precursors, monocyte subsets, mobilisation. Cytometric profiling determined subsets ++ CD16 − , BM, PB synovial fluid (SF) ELISAs quantified...
The global COVID-19 pandemic has led to significant advancements in vaccine research, particularly regarding patients with autoimmune inflammatory rheumatic diseases (AIIRD). However, most studies have assessed the post-vaccination cellular response only by measuring production of interferon-gamma. This study aimed explore immune AIIRD, a focus on effects immunomodulatory drugs different proteins involved and cytotoxicity. We analyzed blood samples from 54 - 16 healthy controls (HC) 38 AIIRD...
MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism genetic and epigenetic regulation Treg Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal was to understand potential impact selected microRNAs profiles on Treg/Th17 frequency, RA phenotype, profile microRNAs, their correlation with factors: SOCS1, SMAD3, SMAD4, STAT3, STAT5 in RA; we used...
TLR9 recognizes unmethylated CpG-rich, pathogen-derived DNA sequences and represents the component of innate immune system that heavily influences adaptive immunity may contribute to immunological disturbances in rheumatoid arthritis (RA). Accumulating data indicate BM RA patients participates pathogenesis this disease as a site proinflammatory cytokines overproduction lymphocytes activation. Here, we investigated functionality its role modulation B-cell functions. We report B cells isolated...
<h3>Objectives</h3> (1) To compare the absolute T-cell numbers in bone marrow (BM) isolated from patients with rheumatoid arthritis (RA) and osteoarthritis (OA); (2) to measure levels of soluble interleukin 15 (IL-15) IL-7; (3) analyse expression activation markers on T cells; (4) influence IL-15 stimulation proliferation. <h3>Methods</h3> BM samples were obtained undergoing joint replacement surgery. Concentrations IL-7 measured using specific ELISAs. The number lymphocytes, their status...
Enhanced/disturbed activities of monocytes are crucial for perpetuation and development rheumatoid arthritis (RA). Therefore, knowledge about monocyte regulation molecular pathways operating within early in the course RA may help to predict progression full-blown disease. We aimed investigate profile miRNAs expression circulating monocyte-related cytokines sera individuals at undifferentiated (UA) stage, wich could serve as new biomarkers development.Magnetically sorted from peripheral blood...
Mesenchymal stem/stromal cells (MSCs) have immunosuppressive and regenerative properties. Adipose tissue is an alternative source of MSCs, named adipose-derived mesenchymal stem (ASCs). Because the biology ASCs in rheumatic diseases (RD) poorly understood, we performed a basic characterization RD/ASCs. The phenotype expression adhesion molecules (intracellular molecule (ICAM)-1 vascular cell (VCAM)-1) on commercially available healthy donors (HD), ASC lines (n = 5) isolated from patients...
Previous studies have shown a reduction in the effectiveness of primary COVID-19 vaccination patients with rheumatic diseases. However, limited data is available regarding vaccine booster dose, especially on cellular response. The study aimed to assess humoral and immunogenicity dose inflammatory arthritis (IA). 49 IA 47 age sex-matched healthy controls (HC) were included prospective cohort study. Both groups completed after more than 180 days received BNT162b2 shot. Humoral responses (level...
Background: Activated T lymphocytes play an important role in the pathogenesis of rheumatic diseases (RD). Mesenchymal stem cells (MSCs) possess immunoregulatory activities but such functions MSCs from bone marrow systemic lupus erythematosus (SLE), sclerosis (SSc), and ankylosing spondylitis (AS) patients are impaired. Adipose tissue–derived (ASCs) optional pool therapeutically useful MSCs, biology these RD is poorly known. This study aimed at investigating effect ASCs healthy donors (HD)...
The domination of pro-inflammatory Th subsets (Th1, Th17) is characteristic ankylosing spondylitis (AS). Mesenchymal stem cells (MSC) were reported to normalize imbalance, but whether MSCs from AS adipose tissue (AS/ASCs) possess such properties unknown. We examined AS/ASCs’ impact on Th-cell differentiation, using healthy donors ASCs (HD/ASCs) as a control. assessment the expression transcription factors defining Th1 (T-bet), Th2 (GATA3), Th17 (RORc), and Treg (FoxP3) by quantitative...
Objectives: Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are chronic wasting, incurable rheumatic diseases of autoimmune background, in which T cells play a critical pathogenic role. Autologous adipose tissue-derived mesenchymal stem (ASCs) may represent an alternative therapeutic option for SLE SSc patients, but the biology these is poorly understood. Methods: Herein, we evaluated anti-proliferative impact ASCs healthy donors (HD/ASCs, 5 reference cell lines), patients (...
To investigate the associations of IL-18 serum levels with lipids, cardiovascular risk, and disease activity in patients ankylosing spondylitis (AS) psoriatic arthritis (PsA) axial (axPsA) peripheral (perPsA) joint involvement.155 adult (PsA 61/AS 94) were enrolled study. Standard indices, BASDAI, ASDAS, calculated for AS PsA DAPSA PsA. Sera from blood samples obtained after night fasting. Serum concentrations cytokines (IL-18, IL-17) measured by ELISA, while lipid profile total cholesterol...
Rheumatoid arthritis (RA) and osteoarthritis (OA) are destructive joint diseases, the development of which associated with expansion pathogenic T lymphocytes. Mesenchymal stem cells may be an attractive therapeutic option for patients RA or OA due to regenerative immunomodulatory abilities these cells. The infrapatellar fat pad (IFP) is a rich easily available source mesenchymal (adipose-derived cells, ASCs). However, phenotypic, potential properties ASCs have not been fully characterised....
This study aimed to investigate the associations of microRNA (miRs) signatures with cytokines, serum lipids, and disease activity in patients psoriatic arthritis (PsA), ankylosing spondylitis (AS), rheumatoid (RA). In total, 65 (PsA n = 25, AS RA 15) 25 healthy controls (HC) were enrolled into study. The expression miR-223-5p, miR-92b-3p, miR-485-3p, miR-10b-5p, let-7d-5p, miR-26a-2-3p, miR-146b-3p, cytokines levels measured sera. DIANA-mirPath analysis was used predict pathways targeted by...
Adipose-derived mesenchymal stem cells (ASCs) have immunoregulatory properties, but their activity is dependent on signals provided by the local microenvironment. It likely that highly inflammatory milieu of rheumatoid joint affects ASCs activity. To test this hypothesis, function derived from articular adipose tissue (AT-ASCs) and subcutaneous (Sc-ASCs) has been analysed. Articular (infrapatellar fat pad) (from site skin closure with sutures) were obtained arthritis (RA) patients undergoing...
Rheumatoid arthritis (RA) is a chronic inflammatory disease leading to joint destruction. In addition involvement of the joints, there growing evidence that inflammatory/autoimmune processes take place in bone marrow, beginning onset. Activated T and B cells accumulate where also effective antigen presentation takes place. An increased number activated was observed RA comparison osteoarthritis (OA) marrow. present study we analyzed levels chemokines may be responsible for...
In ankylosing spondylitis (AS), accompanied by chronic inflammation, T cell expansion plays a pathogenic role; the immunoregulatory properties of bone marrow-derived mesenchymal stem cells (BM-MSCs) are impaired, while functional characteristics their adipose tissue-derived counterparts (ASCs) unknown. We evaluated antiproliferative activity AS/ASCs, obtained from 20 patients, towards allogeneic and autologous lymphocytes, using ASCs healthy donors (HD/ASCs) as reference lines. The...
Complex pathogenesis of systemic lupus erythematosus (SLE) and sclerosis (SSc) is associated with an imbalance various Th-cell subpopulations. Mesenchymal stem cells (MSCs) have the ability to restore this balance. However, bone marrow-derived MSCs SLE SSc patients exhibit many abnormalities, whereas properties adipose derived mesenchymal (ASCS) are much less known. Therefore, we examined effect ASCs obtained from (SLE/ASCs) (SSc/ASCs) on Th subset differentiation, using healthy donors...
Impaired immunogenicity of COVID-19 vaccinations in inflammatory arthritis (IA) patients results diminished immunity. However, optimal booster vaccination regimens are still unknown. Therefore, this study aimed to assess the kinetics humoral and cellular responses IA after booster. In 29 16 healthy controls (HC), (level IgG antibodies) (IFN-γ production) were assessed before (T0), 4 weeks (T1), more than 6 months (T2) from with BNT162b2. patients, but not HC, showed lower anti-S-IgG...
Mesenchymal stromal cells (MSCs) represent a unique cell type with anti-proliferative effects on activated T and B cells. Based our observation of differences between rheumatoid arthritis osteoarthritis bone marrow we hypothesized that MSCs may enhance B-cell survival. We aimed to compare the effect marrow-derived (rheumatoid MSCs, MSCs) survival healthy donor purified Rheumatoid were isolated from patients undergoing hip replacement surgery, cultured in vitro for 2-5 passages. Washed...
Rheumatoid arthritis (RA) is a systemic, autoimmune disease leading to joint destruction and ultimately disability. Bone marrow (BM) an important compartment in RA, where pathological processes from "outside the joint" can occur. IL-17 cytokine that exerts proinflammatory effects participates process of bone destruction. It believed involved pathogenesis RA. However, little known about biology this BM. In present study we investigated Th17-related cytokines RA BM.BM samples were obtained...